切换导航
  • 比较产品
货号直购
我的购物车

若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

main product photo

抗人CD45抗体，克隆HI30

小鼠单克隆IgG1抗体，抗人、黑猩猩CD45
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #60018_C

小鼠单克隆IgG1抗体，抗人、黑猩猩CD45

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

HI30抗体识别CD45的所有亚型，CD45是一种I型跨膜糖蛋白，在大多数血细胞表面表达，除了成熟红细胞、血小板和浆细胞外（这些细胞类型在分化过程中会丢失CD45表达）。CD45是蛋白酪氨酸磷酸酶家族的一员，在许多免疫调节过程中起作用，包括细胞活化、生长、分化和致癌转化。CD45的大细胞质部分包含两个酪氨酸磷酸酶结构域（其中一个具有酶活性），参与调节细胞内底物（如Src激酶Lck和Fyn）的功能。CD45的几种亚型已经被鉴定出来，其表达根据细胞类型和功能状态而不同。编码胞外RA， RB和RC多肽序列的三个外显子（4,5,6）的选择性剪接产生了多达8个分子量在180 - 240 kDa范围内的异构体。白细胞共同抗原（LCA）是HI30抗体识别的区域，该区域位于胞外的膜近端，是所有CD45亚型共同具备的。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™人T细胞富集试剂盒（产品号 #19051），EasySep™人CD4+ T细胞富集试剂盒（产品号 #19052）和EasySep™人CD45去除试剂盒（产品号 #18259；可能观察到部分阻断））分选的细胞纯度。

分类
一抗
 
靶抗原
CD45
 
别名
B220，LCA，T200
 
反应种属
黑猩猩，人
 
偶联
APC，FITC
 
宿主物种
小鼠
 
细胞类型
粒细胞及其亚群，造血干/祖细胞，白细胞，淋巴细胞，髓系细胞
 
种属
人、非人灵长类
 
应用
细胞分选，CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀
 
研究领域
免疫
 
克隆
HI30
 
Gene ID
5788
 
Isotype
IgG1, κ轻链
 

查看更多 显示更少

实验数据

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 6. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 8. Data for PB-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, Pacific Blue™ (filled histogram) or a mouse IgG1, kappa Pacific Blue™ isotype control antibody (solid line histogram).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, Alexa Fluor® 488
Catalog #
60018AD, 60018AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, APC
Catalog #
60018AZ, 60018AZ.1, 100-0467
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, Biotin
Catalog #
60018BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, FITC
Catalog #
60018FI, 60018FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, Pacific Blue™
Catalog #
60018PB.2, 60018PB, 60018PB.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, PE
Catalog #
60018PE, 60018PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, PerCP-Cy5.5
Catalog #
60018PS, 60018PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30
Catalog #
60018
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, Alexa Fluor® 488
Catalog #
60018AD, 60018AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, APC
Catalog #
60018AZ, 60018AZ.1, 100-0467
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, Biotin
Catalog #
60018BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, FITC
Catalog #
60018FI, 60018FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, Pacific Blue™
Catalog #
60018PB.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, Pacific Blue™
Catalog #
60018PB, 60018PB.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, PE
Catalog #
60018PE, 60018PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30, PerCP-Cy5.5
Catalog #
60018PS, 60018PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45 Antibody, Clone HI30
Catalog #
60018
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (4)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

文献 (1)

Enhancement of proliferation of human umbilical cord blood-derived CD34+ hematopoietic stem cells by a combination of hyper-interleukin-6 and small molecules. Y. S. Park et al. Biochemistry and biophysics reports 2022 mar

Abstract

Umbilical cord blood (UCB) is an alternative source of allogeneic hematopoietic stem cells (HSCs) for transplantation to treat various hematological disorders. The major limitation to the use of UCB-derived HSCs (UCB-HSCs) in transplantation, however, is the low numbers of HSCs in a unit of cord blood. To overcome this limitation, various cytokines or small molecules have been used to expand UCB-HSCs ex vivo. In this study, we investigated a synergistic effect of the combination of HIL-6, SR1, and UM171 on UCB-HSC culture and found that this combination resulted in the highest number of CD34+ cells. These results suggest that the combination of SR1, UM171 and HIL-6 exerts a synergistic effect in the proliferation of HSCs from UCB and thus, SR1, UM171 and HIL-6 is the most suitable combination for obtaining HSCs from UCB for clinical transplantation.
View publication
View All Publications

更多信息

更多信息
种属 Human, Non-Human Primate
克隆 HI30
Gene Id 5788
Alternative Names B220, LCA, T200
同种型/isotype IgG1, kappa
法律声明:

Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. 质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
Copyright © 2025 by STEMCELL Technologies. All rights reserved.