小鼠 IgG1，kappa 同型对照抗体（克隆 MOPC-21）

小鼠单克隆IgG1， kappa同型对照抗体

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产品号 #（选择产品）

产品号 #60070_C

小鼠单克隆IgG1， kappa同型对照抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

MOPC-21 抗体 (IgG1, kappa) 适合在多种应用中用作同型对照抗体，以评估抗原特异性抗体的非特异性结合程度。理想情况下，同型对照应与所用特异性抗体具有相同的重链亚类（IgA、IgD、IgE、IgG 或 IgM）和轻链亚类（kappa 或 lambda）。如果使用偶联抗体，则应选择与相同分子（例如荧光染料）偶联的同型对照。使用合适的同型对照有助于确认抗原特异性抗体的特异性，并指示可能由与 Fc 受体或其他细胞成分结合引起的非特异性结合。
该抗体克隆已验证可用作同型对照抗体，用于评估流式细胞术和免疫荧光显微镜应用（表面和细胞内染色）等应用中的细胞非特异性结合。

亚型
同型对照抗体

靶抗原
IgG1 Isotype Control

别名
Not applicable

偶联
Alexa Fluor 488，APC，FITC，PE，未偶联的

宿主物种
小鼠

细胞类型
其它细胞系

种属
人，小鼠，非人灵长类，其它细胞系，大鼠

应用
流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹

研究领域
免疫

克隆
MOPC-21

基因编号
Not applicable

同种型
IgG1，kappa

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实验数据

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, Catalog #60107) followed by a rat anti-mouse IgG1 antibody, PE.(B) Human embryonic stem (ES) cells were cultured using mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, followed by goat anti-mouse IgG, FITC (inset) or with a positive control antibody of the same isotype, Anti-Human SSEA-5 Antibody, Clone 8e11 (Catalog #60063), followed by goat anti-mouse IgG, FITC (green).

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, Alexa Fluor® 488, Catalog #60107AD). (B) Human induced pluripotent stem (iPS) cells were cultured using mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (inset) or with a positive control antibody of the same isotype, Anti-Human SSEA-5 Antibody, Clone 8e11, Alexa Fluor® 488 (Catalog #60063AD, green).

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, APC, Catalog #60107AZ).

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, Biotin, Catalog #60107BT) followed by SAV APC.

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, FITC, Catalog #60107FI).

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, PE, Catalog #60107PE). (B) Human induced pluripotent stem (iPS) cells were cultured using mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (inset) or with a positive control antibody of the same isotype, Anti-Human SSEA-5 Antibody, Clone 8e11, PE (Catalog #60063PE, red).

Figure 7. Data for PS-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC- 21, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD45 Antibody, Clone HI30, PerCP-Cy5.5; Catalog #60018PS).