小鼠IgG2a，kappa同型对照抗体，克隆C1.18.4

小鼠单克隆IgG2a， kappa同型对照抗体

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产品号 #60108_C

小鼠单克隆IgG2a， kappa同型对照抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

C1.18.4抗体（IgG2a, kappa）适合作为同型匹配对照抗体，用于在多种应用中估计抗原特异性抗体的非特异性结合程度。理想情况下，同型对照的重链（IgA、IgD、IgE、IgG或IgM）和轻链（kappa或lambda）的亚类应与所使用的特异性抗体相同。如果采用偶联抗体，则应选择偶联相同分子（如荧光染料）的同型对照。使用适当的同型对照有助于确认抗原特异性抗体的特异性，并显示出可能由抗体结合Fc受体或结合其他细胞成分导致的非特异性结合。

该抗体克隆已被验证可作为同型对照抗体，用于在流式细胞术等应用中评估与细胞的非特异性结合。

亚型
同型对照抗体

靶抗原
IgG2a Isotype Control

别名
Not applicable

偶联
APC，FITC，PE，PerCP-Cyanine5.5，未偶联的

宿主物种
小鼠

细胞类型
其它细胞系

种属
人，小鼠，非人灵长类，其它细胞系，大鼠

应用
流式细胞术，功能学筛选，免疫组化，Western印迹

研究领域
免疫

克隆
C1.18.4

基因编号
Not applicable

同种型
IgG2a，kappa

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实验数据

Figure 1. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, followed by a rat anti-mouse IgG2a antibody, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, Catalog #60097) followed by a rat anti-mouse IgG2a antibody, PE.

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, APC, Catalog #60097AZ).

Figure 3. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, FITC, Catalog #60097FI).

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, PE, Catalog #60097PE).

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5, Catalog #60097PS).