抗人CD56抗体，克隆HCD56

小鼠单克隆IgG1抗体，抗人CD56 （NCAM）

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产品号 #60021_C

小鼠单克隆IgG1抗体，抗人CD56 （NCAM）

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总览

实验数据

产品说明书及文档

应用领域

总览

HCD56抗体识别CD56 （NCAM）反应，CD56 （NCAM）是免疫球蛋白（Ig）超家族的成员，在其细胞外部分含有5个Ig样结构域和两个3型纤连蛋白结构域。CD56已被鉴定出多个亚型，包括跨膜和gpi锚定亚型。CD56受到几种类型的翻译后修饰，包括在第5个Ig样结构域添加多聚糖酸片段。CD56的~140 kDa亚型被认为是人NK细胞的原型细胞表面标志物。约90%的NK细胞低密度表达CD56，而约10%的NK细胞相对高表达CD56。CD56也在外周血NKT亚群、神经元、肌肉细胞、一些干细胞和体外培养的T细胞克隆中呈可变表达。CD56也在几种类型的肿瘤中表达，包括骨髓瘤、神经母细胞瘤和小细胞肺癌细胞。CD56分别通过嗜同性和嗜异性相互作用介导细胞-细胞和细胞-基质的粘附，并参与激活细胞内信号通路。CD56在胚胎和成体的神经元发育中都起到重要作用。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™HLA Buffy Coat CD56正选试剂盒（产品号 #18085HLA）和EasySep™Human CD56正选试剂盒（产品号#18055，可能观察到部分阻断），以及EasySep™HLA CD2正选试剂盒（产品号 #18657HLA）和EasySep™人NK细胞富集试剂盒（产品号 #19055）分选的细胞纯度。

亚型
一抗

靶抗原
CD56 (NCAM)

别名
Leu-19，N-CAM，NCAM-1，NKH1

活性物种
人

偶联
Alexa Fluor 488，APC，Biotin 或生物素，FITC，PE，未偶联的

宿主物种
小鼠

细胞类型
NK 细胞

种属
人

应用
CyTOF，流式细胞术，免疫组化

研究领域
免疫

克隆
HCD56

基因编号
4684

同种型
IgG1，kappa

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实验数据

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD56 Antibody, Clone HCD56, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC and Anti-Human CD45 Antibody, Clone HI30, APC.

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD56 Antibody, Clone HCD56, Alexa Fluor® and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD56 Positive Selection Kit and labeled with Anti-Human CD56 Clone HCD56, Alexa Fluor® 488. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody is shown (solid line histogram).

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD56 Antibody, Clone HCD56, APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa APC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, PE. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Buffy Coat CD56 Positive Selection Kit and labeled with Anti-Human CD56 Antibody, Clone HCD56, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD56 Antibody, Clone HCD56, Biotin, followed by streptavidin (SAV) APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC and Anti-Human CD45 Antibody, Clone HI30, PE. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Buffy Coat CD56 Positive Selection Kit and labeled with Anti-Human CD56 Antibody, Clone HCD56, Biotin followed by SAV APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC is shown (solid line histogram).

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD56 Antibody, Clone HCD56, FITC and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa FITC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, APC. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Buffy Coat CD56 Positive Selection Kit and labeled with Anti-Human CD56 Antibody, Clone HCD56, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD56 Antibody, Clone HCD56, PE and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD56 Positive Selection Kit and labeled with Anti-Human CD56 Antibody, Clone HCD56, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).