JavaScript 似乎在您的浏览器中被禁用。 为了您在我们网站能获得最好的体验，建议在浏览器中打开Javascript。

若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

HepatiCult™ 类器官生长培养基 (人)

用于长期扩增和维持人肝脏类器官的培养基

率先评论此产品

产品号 #（选择产品）

产品号 #100-0385_C

用于长期扩增和维持人肝脏类器官的培养基

产品组分包括

HepatiCult™ 类器官基础培养基（人），95 mL
HepatiCult™ 类器官生长补充剂（人），5 mL

跳到结尾的图片库

跳转到图像库的开头

Interested in trying STEMCELL's organoid products for your liver research? Fill out this form to request information about introductory offers.

要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

Trypan Blue
Reagent for counting viable mammalian cells
Ammonium Chloride Solution
Reagent for lysis of red blood cells
Costar® 24-Well Flat-Bottom Plate, Tissue Cu...
Clear polystyrene flat-bottom, tissue culture-treated multiwell cell culture plate with lid
Y-27632 (Dihydrochloride)
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

稳定扩增并维持人肝脏类器官，作为研究肝脏生物学、疾病模型、药物发现和治疗筛选的模型系统。使用 HepatiCult™ 类器官生长培养基（OGM）（人）可从人肝组织或通过 STEMdiff™ 肝细胞试剂盒生成的 PSC 衍生肝细胞建立肝脏类器官。

HepatiCult™ OGM可单独购买，也可作为 HepatiCult™ 类器官培养基（人）的组成部分，用于培养和扩增肝脏类器官，以供后续实验或生物样本库保存使用。在 HepatiCult™ 类器官启动培养基（人）中建立，并在 HepatiCult™ OGM 中扩增的类器官表达自我更新（LGR5、AXIN2）、导管（KRT19、SOX9、KRT7）和肝祖细胞（HNF4A）的相关标志物。这些类器官还可以使用 HepatiCult™ 类器官分化培养基（人）分化为成熟类器官，表现出包括 CYP3A4 活性在内的肝脏功能。

使用 HepatiCult™ OGM 可用多种形式培养类器官，包括用于常规培养的 Corning® Matrigel® 胶滴、用于规模化扩增的稀释 Matrigel® 悬液（置于旋转瓶中）、用于高通量筛选应用的 96 孔板，以及用于疾病建模的 Transwell® 插件上的类器官来源的二维单层细胞。这些类器官还可以冷冻保存以供后续使用，解冻后在 HepatiCult™ OGM 中培养，仍能保持较高的活率，并具有进一步分化的能力。

有关使用 HepatiCult™ 进行类器官培养的更多信息，请参阅技术手册和教育材料。

如果您打算将此产品用于商业目的，请通过www.huborganoids.nl联系HUB以获得商业使用许可或有关HUB许可的说明。

有关使用 HepatiCult™ 进行类器官培养的更多信息，请参阅技术手册和教育材料。

如果您打算将此产品用于商业目的，请通过www.huborganoids.nl联系HUB以获得商业使用许可或有关HUB许可的说明。

有关使用 HepatiCult™ 进行类器官培养的更多信息，请参阅技术手册和教育材料。

如果您打算将此产品用于商业目的，请通过www.huborganoids.nl联系HUB以获得商业使用许可或有关HUB许可的说明。

有关使用 HepatiCult™ 进行类器官培养的更多信息，请参阅技术手册和教育材料。

如果您打算将此产品用于商业目的，请通过www.huborganoids.nl联系HUB以获得商业使用许可或有关HUB许可的说明。

亚型
专用培养基

细胞类型
肝细胞

种属
人

应用
类器官培养

品牌
HepatiCult

研究领域
疾病建模，药物发现和毒理检测，上皮细胞研究，类器官，干细胞生物学

制剂类别
无血清

查看更多显示更少

实验数据

Figure 1. HepatiCult™ Organoid Kit (Human) Enables Liver Organoid Initiation, Expansion, and Differentiation

Human liver organoids can be grown from normal human liver tissue-derived hepatic ducts using the HepatiCult™ Organoid Kit (Human). (A) Cultures are established in HepatiCult™ Organoid Initiation Medium (OIM; Human) or HepatiCult™ Organoid Growth Medium (OGM; Human) (see Table 1 below) and subsequently passaged in HepatiCult™ OGM for expansion. (B) After passaging 2-3 times in HepatiCult™ OGM, cultures can be switched to HepatiCult™ Organoid Differentiation Medium (ODM; Human) to differentiate organoids towards more mature hepatic cell types. Refer to the Product Manual (Document #10000008301) for full culturing protocols.

Table 1. Product Recommendations for Liver Organoid Initiation, Expansion, and Differentiation

The recommended configuration of the HepatiCult™ Organoid Kit (Human) may differ based on starting material and experimental goals. When establishing liver organoid cultures from human liver tissue, HepatiCult™ Organoid Initiation Medium (OIM; Human) is recommended for efficient organoid initiation (see Figure 2 below). The expansion of already established organoids (fresh in culture or cryopreserved) is supported by HepatiCult™ Organoid Growth Medium (OGM; Human). These organoids should be maintained for 2-3 passages before further differentiation using HepatiCult™ Organoid Differentiation Medium (ODM). Refer to the Product Manual (Document #10000008301) for full culturing protocols.

Figure 2. HepatiCult™ Organoid Kit (Human) Provides Efficient Organoid Initiation From Human Liver Tissue

(A) Organoid cultures were initiated in HepatiCult™ OIM, and then (B) passaged into HepatiCult™ OGM. For serum-free culture conditions, organoid cultures were both, (C) initiated in and (D) passaged in HepatiCult™ OGM. Culture images shown are from (A, C) day 15 following initiation, and (B, D) on day 8 of the first passage. (E) Quantification of organoid initiation efficiency shows a significantly higher organoid yield in HepatiCult™ OIM per gram of human liver tissue (mean ± SD; n=14).

Figure 3. HepatiCult™ Organoid Initiation Medium (Human) Supports Robust Organoid Establishment Across Multiple Liver Tissue Donor Samples

Organoids initiated from 4 donor tissue samples (A-D) exhibit morphological heterogeneity 15 days after initiation. All initiated cultures were subsequently expanded in HepatiCult™ OGM using a 1:1 passaging ratio (E-H), yielding healthy organoids at the end of the first passage.

Figure 4. Expansion of Organoid Cultures in HepatiCult™ Organoid Growth Medium

Hepatic organoids show efficient growth in HepatiCult™ OGM across multiple donors and passages with potential for indefinite culture. Organoids initiated using the serum-free workflow exhibited comparable growth rates when expanded in HepatiCult™ OGM (open markers).

Figure 5. Proliferating Hepatic Organoids Display Characteristics of Hepatic Progenitors

Human liver organoids grown in HepatiCult™ OGM display characteristics of proliferating hepatic progenitors observed through immunocytochemistry staining of (A) KI67, (B) HNF4A and (C) SOX9. Proliferating hepatic organoids also display characteristics of the hepatic epithelium including expression of (A) EPCAM. (B, C) Nuclei are counterstained with DAPI.

Figure 6. Proliferating Hepatic Organoids Maintain Genetic Expression Across Multiple Passages

Liver organoids maintained in HepatiCult™ OGM express stem cell markers (A) LGR5 and (B) AXIN2, ductal markers (C) SOX9 and (D) KRT19, as well as hepatic marker (E) HNF4a and (F) Albumin (ALB) across multiple passages, with minimal albumin expression observed during culture in HepatiCult™ OGM. Expression levels were measured by qPCR and normalized to TBP and UBC housekeeping genes to quantify relative expression levels. (mean ± SD; n = 2-5 organoid lines), * p < 0.05.

Figure 7. Organoid Differentiation Induces Changes in Organoid Morphology

Organoids exhibit a compact and dense morphology, often comprising thickened epithelia, upon switching cultures to HepatiCult™ Organoid Differentiation Medium (ODM). Shown are images of the same culture well over the course of the differentiation, including (A) day two of culture in HepatiCult™ OGM, (B) day five of culture, immediately after switching organoid cultures from HepatiCult™ OGM to HepatiCult™ ODM, (C) day seven of culture (two days after switching to ODM), (D) day ten of culture (five days after switching to ODM), and (E) day 15 of culture (ten days after switching to ODM). (F) Magnification of the rectangular section highlighted in (E).

Figure 8. Differentiation of Hepatic Organoids in HepatiCult™ ODM Induces Changes in Gene Expression Consistent With Hepatic Maturation

Upon differentiation in HepatiCult™ ODM, liver organoids show changes in gene expression with a decrease in expression of stem cell marker LGR5, as well as of ductal markers CK19, CK7, and SOX9, and an increase in expression of hepatic markers HNF4A, ALB, CYP3A4, and ASGR1. Each replicate represents an individual donor sample analyzed by qPCR at passage 4 on day 8 in HepatiCult™ OGM and day 15 in HepatiCult™ ODM, showing relative gene expression levels normalized to TBP and UBC housekeeping genes.

Figure 9. Differentiated Hepatic Organoids Demonstrate Functionality of Mature Hepatocytes

Upon differentiation in HepatiCult™ ODM, liver organoids were assayed for (A) albumin secretion, (B) CYP3A4 activity, (C) total bile acid production, and (D) urea production. Hepatic functionalities were compared to HepG2 cells and primary human hepatocytes (PHH), which were cultured in supplier-recommended media. Albumin secretion was detected using an ELISA kit (Abcam), total bile acid and urea production were analysed using colorimetric kits (Abcam), and CYP43A4 activity, referring to baseline activity without induction, was determined using the Luciferin-IPA kit (Promega). (mean ± SD; n = 3 organoid lines across 2 experiments, n = 2-3 technical replicates of HepG2 in 1 experiment, and n = 3 cryopreserved PHH donor samples in 1 experiment), * p < 0.05; ** p < 0.01.

Figure 10. HepatiCult™ Organoid Kit Supports the Growth and Differentiation of Porcine Liver Organoids

Porcine liver organoids were established in HepatiCult™ Organoid Initiation Medium, and subsequently expanded for 4 passages in (A) HepatiCult™ Organoid Growth Medium (Human) and differentiated in (B, C) HepatiCult™ Organoid Differentiation Medium (Human). Organoids are stained with basolateral protein marker p120 (green), apical membrane and bile canaliculi marker F-Actin (purple), and nuclear dye Hoechst (blue). (D) Magnification of Panel C indicates bile canaliculi formation as seen through F-Actin staining. Scale bars = 50 μm. Data used with permission from Dr. Amy Engevik (Vanderbilt University Medical Center)