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ImmunoCult™ 人Treg分化添加剂

人naïve CD4+ T细胞向调节性T细胞(Tregs)分化的无血清培养添加剂

产品号 #(选择产品)

产品号 #10977_C

人naïve CD4+ T细胞向调节性T细胞(Tregs)分化的无血清培养添加剂

产品优势

  • 专为诱导人Treg细胞分化而配制
  • 优化用于搭配ImmunoCult™-XF T细胞扩增培养基
  • 较竞品Treg分化试剂盒相比,体现更高占比的记忆T细胞
  • CD4+CD25+FOXP3+T细胞占比较竞品Treg分化试剂盒高出多达20%
  • 以50倍浓缩液形式提供;解冻并混匀后,管内容物可直接添加至培养基中

总览

ImmunoCult™人Treg分化添加剂含有人源细胞因子和小分子,旨在促进外周血来源的人初始CD4+T细胞高效稳定激活、扩增并分化为Treg细胞。ImmunoCult™人Th2分化添加剂可与ImmunoCult™-XF T细胞扩增培养基(产品号 #10981)和ImmunoCult™人CD3/CD28 T细胞激活剂(产品号 #10971)搭配使用。

本产品推荐用于:

•研究人初始T细胞分化和Treg功能的调节

•开发初始T细胞扩增及向Treg极化的工艺流程

本产品推荐用于:

•研究人初始T细胞分化和Treg功能的调节

•开发初始T细胞扩增及向Treg极化的工艺流程

本产品推荐用于:

•研究人初始T细胞分化和Treg功能的调节

•开发初始T细胞扩增及向Treg极化的工艺流程

本产品推荐用于:

•研究人初始T细胞分化和Treg功能的调节

•开发初始T细胞扩增及向Treg极化的工艺流程

包含
• Recombinant human TGF-β1 • All-trans retinoic acid
 
亚型
添加剂
 
细胞类型
T 细胞,T 细胞,CD4+
 
种属

 
应用
细胞培养,分化,扩增
 
研究领域
免疫
 

实验数据

Figure 1. ImmunoCult™ Human Treg Differentiation Supplement Produces Differentiated CD4+CD45RA-CD45RO+ Cells Under Treg Polarizing Conditions

Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555). Cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Treg Differentiation Supplement (Catalog #10977), cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) for 7 days. Shown are flow cytometry results of cell samples stained and analyzed for expression of CD45RA and CD45RO (A) before and (B) after 7 days of culture. After 7 days the majority of Treg cells in culture display a memory T cell phenotype (CD45RA-CD45RO+).

Figure 2. ImmunoCult™ Human Treg Differentiation Supplement Produces Similar Numbers of Differentiated CD4+CD45RA-CD45RO+ Cells Under Treg Polarizing Conditions Than Competitor

Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) and cultured with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Treg Differentiation Supplement (Catalog #10977) in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) (purple), or competitor medium and activator (grey) for 5 days (based on the length of the competitor protocol). The percentage of naïve (CD45RA+CD45RO-) and differentiated (CD45RA-CD45RO+) cells after culture was compared after staining cells for CD45RA and CD45RO and analysis by flow cytometry. The percentage of cells expressing a naïve phenotype and the percentage of cells expressing a differentiated phenotype were not significantly higher with ImmunoCult™ than with competitor medium and activator. Shown are the differences in the percentage of naïve and differentiated T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; paired t-test on logit transformed data; n = 4).

Figure 3. ImmunoCult™ Human Treg Differentiation Supplement Produces FOXP3+ Cells Under Treg Polarizing Conditions

Naïve CD4+ T cells were cultured for 7 days in ImmunoCult™ activator, supplement and medium using the protocol described in Figure 2. Cells were stained for CD4, CD4, CD25 and FOXP3 and analyzed on days 0, 4, 5 and 7 by flow cytometry. Each bar represents the mean percentage of CD3+CD4+ cells expressing both CD25 and FOXP3 (CD3+CD4+CD25+FOXP3+) (mean ± S.E.M; n = 4 – 20 donors).

Figure 4. Culture with ImmunoCult™ Human Treg Differentiation Supplement Produces Similar Percentages of CD3+CD4+CD25+FOXP3+ Cells as Culture Under Competitor Conditions

Naïve CD4+ T cells were cultured for 5 days in ImmunoCult™ activator, supplement and medium, or competitor medium and activator using the protocol described in Figure 2. The percentage of CD25+FOXP3+ after 5 days of culture was compared after staining cells for CD3, CD4, CD25 and FOXP3 and analysis by flow cytometry. Similar percentages of CD3+CD4+ cells generated with ImmunoCult™ expressed CD25 and FOXP3 as compared to those generated under competitor conditions. Shown are the differences in the percentage of CD3+CD4+ cells expressing both CD25 and FOXP3 (CD3+CD4+CD25+FOXP3+) in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; paired t-test on logit transformed data; n = 4 - 20).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
10977
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
10977
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

更多信息

更多信息
种属 Human
Contains • Recombinant human TGF-β1 • All-trans retinoic acid
质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息,请访问 STEMCELL.CN/COMPLIANCE. Safety Statement: CA WARNING: This product can expose you to All-trans Retinoic Acid which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov
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