产品号 #10957_C
小鼠naïve CD4+ T细胞向调节性T细胞(Tregs)细胞分化的无血清培养补充
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ImmunoCult™小鼠Treg分化补充剂包含重组小鼠和人细胞因子的组合以及小分子,优化用于促进小鼠naïve CD4+ T细胞向调节性T细胞(Tregs)分化。该补充剂与含有胎牛血清和其他添加剂的RPMI 1640培养基(产品号#36750)以及抗小鼠CD3和抗小鼠CD28单克隆抗体作为活化剂体系搭配使用。
本产品推荐用于:
•研究小鼠naïve T细胞分化和Treg功能的调节
•开发将naïve T细胞扩增以及向Treg亚群极化的工艺流程
本产品推荐用于:
•研究小鼠naïve T细胞分化和Treg功能的调节
•开发将naïve T细胞扩增以及向Treg亚群极化的工艺流程
本产品推荐用于:
•研究小鼠naïve T细胞分化和Treg功能的调节
•开发将naïve T细胞扩增以及向Treg亚群极化的工艺流程
本产品推荐用于:
•研究小鼠naïve T细胞分化和Treg功能的调节
•开发将naïve T细胞扩增以及向Treg亚群极化的工艺流程
包含
• Recombinant mouse interleukin 2 (IL-2)
• Recombinant human transforming growth factor beta (TGF-β)
• All-trans retinoic acid (ATRA)
亚型
添加剂
细胞类型
T 细胞,T 细胞,CD4+,T 细胞,调节性细胞
种属
小鼠
应用
细胞培养,分化,扩增
品牌
ImmunoCult
研究领域
免疫
Figure 1. ImmunoCult™ Mouse Treg Differentiation Supplement Produces CD4+CD25+FOXP3+ Cells Under Treg Polarizing Conditions
Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone (Non-polarizing cultures), or in medium supplemented with ImmunoCult™ Mouse Treg Differentiation Supplement (Polarizing cultures) for 6 days. Cells were subsequently stained with anti-CD4, anti-CD25, anti-FOXP3 and a viability dye and analyzed by flow cytometry. Shown is the expression of CD25 and FOXP3 back-gated on viable, CD4+ cells from non-polarized (A) or polarized cultures (B). The mean proportion of CD4+FOXP3+ cells is significantly higher in cells cultured in ImmunoCult™ Mouse Treg Differentiation Supplement (91 ± 2%) compared to non-polarized cells (2 ± 0.4%) (p < 0.001; n = 14). Data from experimental groups were compared using a paired T-test.
Figure 2. ImmunoCult™ Mouse Treg Differentiation Supplement Expands Viable CD4+ Cells Under Treg Polarizing Conditions
Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone, or medium containing ImmunoCult™ Mouse Treg Differentiation Supplement for 6 days. A 3.6-fold expansion of total nuclear cells (TNC) ensues in cultures polarized with ImmunoCult Mouse Treg Differentiation Supplement (purple column), which is significantly higher compared to non-polarized cultures (grey column). (Data represent the mean ± SEM, n = 14, ***p < 0.001. Data from experimental groups were compared using a paired T-test).
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种属 | Mouse |
---|---|
Contains | • Recombinant mouse interleukin 2 (IL-2) • Recombinant human transforming growth factor beta (TGF-β) • All-trans retinoic acid (ATRA) |
小鼠naïve CD4+ T细胞向Th2细胞分化的无血清培养补充
小鼠Naïve CD4+ T细胞的免疫磁珠负选
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