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ImmunoCult™ 小鼠Treg分化添加剂

小鼠naïve CD4+ T细胞向调节性T细胞（Tregs）细胞分化的无血清培养补充
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产品号 #（选择产品）

产品号 #10957_C

小鼠naïve CD4+ T细胞向调节性T细胞（Tregs）细胞分化的无血清培养补充

产品优势

  • 为诱导从C57BL/6小鼠脾脏分选的初始CD4+T细胞向Tregs分化而优化；
  • 作为100X浓缩物供应；解冻和混匀后，管内容物可直接添加到培养基中
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

ImmunoCult™小鼠Treg分化补充剂包含重组小鼠和人细胞因子的组合以及小分子，优化用于促进小鼠naïve CD4+ T细胞向调节性T细胞（Tregs）分化。该补充剂与含有胎牛血清和其他添加剂的RPMI 1640培养基（产品号#36750）以及抗小鼠CD3和抗小鼠CD28单克隆抗体作为活化剂体系搭配使用。

本产品推荐用于：

•研究小鼠naïve T细胞分化和Treg功能的调节

•开发将naïve T细胞扩增以及向Treg亚群极化的工艺流程

本产品推荐用于：

•研究小鼠naïve T细胞分化和Treg功能的调节

•开发将naïve T细胞扩增以及向Treg亚群极化的工艺流程

本产品推荐用于：

•研究小鼠naïve T细胞分化和Treg功能的调节

•开发将naïve T细胞扩增以及向Treg亚群极化的工艺流程

本产品推荐用于：

•研究小鼠naïve T细胞分化和Treg功能的调节

•开发将naïve T细胞扩增以及向Treg亚群极化的工艺流程

包含
• Recombinant mouse interleukin 2 (IL-2) • Recombinant human transforming growth factor beta (TGF-β) • All-trans retinoic acid (ATRA)
 
亚型
添加剂
 
细胞类型
T 细胞，T 细胞，CD4+，T 细胞，调节性细胞
 
种属
小鼠
 
应用
细胞培养，分化，扩增
 
品牌
ImmunoCult
 
研究领域
免疫
 

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实验数据

Figure 1. ImmunoCult™ Mouse Treg Differentiation Supplement Produces CD4+CD25+FOXP3+ Cells Under Treg Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone (Non-polarizing cultures), or in medium supplemented with ImmunoCult™ Mouse Treg Differentiation Supplement (Polarizing cultures) for 6 days. Cells were subsequently stained with anti-CD4, anti-CD25, anti-FOXP3 and a viability dye and analyzed by flow cytometry. Shown is the expression of CD25 and FOXP3 back-gated on viable, CD4+ cells from non-polarized (A) or polarized cultures (B). The mean proportion of CD4+FOXP3+ cells is significantly higher in cells cultured in ImmunoCult™ Mouse Treg Differentiation Supplement (91 ± 2%) compared to non-polarized cells (2 ± 0.4%) (p < 0.001; n = 14). Data from experimental groups were compared using a paired T-test.

Figure 2. ImmunoCult™ Mouse Treg Differentiation Supplement Expands Viable CD4+ Cells Under Treg Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone, or medium containing ImmunoCult™ Mouse Treg Differentiation Supplement for 6 days. A 3.6-fold expansion of total nuclear cells (TNC) ensues in cultures polarized with ImmunoCult Mouse Treg Differentiation Supplement (purple column), which is significantly higher compared to non-polarized cultures (grey column). (Data represent the mean ± SEM, n = 14, ***p < 0.001. Data from experimental groups were compared using a paired T-test).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Mouse Treg Differentiation Supplement
Catalog #
10957
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™ Mouse Treg Differentiation Supplement
Catalog #
10957
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

更多信息

更多信息
种属 Mouse
Contains • Recombinant mouse interleukin 2 (IL-2) • Recombinant human transforming growth factor beta (TGF-β) • All-trans retinoic acid (ATRA)
质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE. Safety Statement: CA WARNING: This product can expose you to All-trans Retinoic Acid which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov
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