切换导航
  • 比较产品
我的购物车

若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

EasySep™小鼠Naïve CD4+ T细胞分选试剂盒

小鼠Naïve CD4+ T细胞的免疫磁珠负选
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #19765_C

小鼠Naïve CD4+ T细胞的免疫磁珠负选

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达95%
  • 获得的活细胞无标记 

产品组分包括

  • EasySep™小鼠Naïve CD4+ T细胞分选试剂盒（产品号 #19765）
    • EasySep™小鼠CD4+ T细胞分选抗体混合物，0.5 mL
    • EasySep™小鼠记忆T细胞去除抗体混合物，0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠 ，1 mL
    • EasySep™小鼠FcR阻断剂，0.1 mL
  • RoboSep™小鼠Naïve CD4+ T细胞分选试剂盒（产品号 #19765RF）
    • EasySep™小鼠CD4+ T细胞分选抗体混合物，0.5 mL
    • EasySep™小鼠记忆T细胞去除抗体混合物，0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠，1 mL
    • EasySep™小鼠FcR阻断剂，0.1 mL
    • RoboSep™缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）
main product photo
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™小鼠初始CD4+ T细胞分选试剂盒，可通过免疫磁珠负选轻松高效地从小鼠脾细胞中分离高纯度初始CD4+ T细胞（CD4+CD44lowCD62Lhigh）。当使用其它类型组织来源的单细胞悬液时，该试剂盒可能需要优化。EasySep™结合了单克隆抗体的特异性和无柱磁性系统的简便性，迄今已广泛应用于发表的研究中超过20年。

在此EasySep™负选过程中，非目的细胞通过抗体复合物与磁珠标记。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的初始CD4+ T细胞（CD4+CD44lowCD62Lhigh）细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的试管中即可。仅需15分钟磁珠分选后， 获得的初始CD4+ T细胞可直接用于流式细胞术、细胞培养或基于细胞的实验 等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选 。探索更多优化您实验流程的产品，包括培养基、添加剂、抗体等。

在此EasySep™负选过程中，非目的细胞通过抗体复合物与磁珠标记。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的初始CD4+ T细胞（CD4+CD44lowCD62Lhigh）细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的试管中即可。仅需15分钟磁珠分选后， 获得的初始CD4+ T细胞可直接用于流式细胞术、细胞培养或基于细胞的实验 等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选 。探索更多优化您实验流程的产品，包括培养基、添加剂、抗体等。

在此EasySep™负选过程中，非目的细胞通过抗体复合物与磁珠标记。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的初始CD4+ T细胞（CD4+CD44lowCD62Lhigh）细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的试管中即可。仅需15分钟磁珠分选后， 获得的初始CD4+ T细胞可直接用于流式细胞术、细胞培养或基于细胞的实验 等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选 。探索更多优化您实验流程的产品，包括培养基、添加剂、抗体等。

在此EasySep™负选过程中，非目的细胞通过抗体复合物与磁珠标记。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的初始CD4+ T细胞（CD4+CD44lowCD62Lhigh）细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的试管中即可。仅需15分钟磁珠分选后， 获得的初始CD4+ T细胞可直接用于流式细胞术、细胞培养或基于细胞的实验 等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选 。探索更多优化您实验流程的产品，包括培养基、添加剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
T 细胞，T 细胞，CD4+
 
种属
小鼠
 
样本来源
其它细胞系，Spleen
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

查看更多 显示更少

实验数据

Typical EasySep™ Mouse Naïve CD4+ T Cell Isolation Profile

Figure 1. Typical EasySep™ Mouse Naïve CD4+ T Cell Isolation Profile

Starting with mouse splenocytes from an uninfected mouse, the naïve CD4+ T cell content (CD4+CD44lowCD62Lhigh) of the isolated fraction typically ranges from 87.6 - 95.6%. In the above example, the purities of the start and final isolated fractions are 14.3% and 94.7%, respectively.

CD4+ Mouse Splenocytes Labeled with PE-Conjugated, Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14

Figure 2. CD4+ Mouse Splenocytes Labeled with PE-Conjugated, Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE (Catalog #60109PE; filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE) (solid line histogram). (B) Flow cytometry analysis of mouse splenocytes (gated on CD4+ cells) processed with EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Catalog #19765) and labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE and an anti-mouse CD44 antibody, FITC. (C) Labeling of the Start splenocytes prior to cell isolation.

ImmunoCult™ Mouse Treg Differentiation Supplement Produces CD4+CD25+FOXP3+ Cells Under Treg Polarizing Conditions

Figure 3. ImmunoCult™ Mouse Treg Differentiation Supplement Produces CD4+CD25+FOXP3+ Cells Under Treg Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone (Non-polarizing cultures), or in medium supplemented with ImmunoCult™ Mouse Treg Differentiation Supplement (Catalog #10957; polarizing cultures) for 6 days. Cells were subsequently stained with anti-CD4, anti-CD25, anti-FOXP3, and a viability dye and analyzed by flow cytometry. Shown is the expression of CD25 and FOXP3 back-gated on viable CD4+ cells from non-polarized (A) or polarized cultures (B). The mean proportion of CD4+FOXP3+ cells is significantly higher in cells cultured in ImmunoCult™ Mouse Treg Differentiation Supplement (91 ± 2%) compared to non-polarized cells (2 ± 0.4%) (p < 0.001; n = 14). Data from experimental groups were compared using a paired T-test.

ImmunoCult™ Mouse Treg Differentiation Supplement Expands Viable CD4+ Cells Under Treg Polarizing Conditions

Figure 4. ImmunoCult™ Mouse Treg Differentiation Supplement Expands Viable CD4+ Cells Under Treg Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28, and cultured in medium alone, or medium containing ImmunoCult™ Mouse Treg Differentiation Supplement (Catalog #10957) for 6 days. A 3.6-fold expansion of total nuclear cells (TNC) occurs in cultures polarized with ImmunoCult™ Mouse Treg Differentiation Supplement (purple column), which is significantly higher compared to non-polarized cultures (grey column). (Data represent the mean ± SEM, n = 14, ***p < 0.001. Data from experimental groups were compared using a paired T-test).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765RF
Lot #
1000116887 or higher
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765
Lot #
1000116887 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
RoboSep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
RoboSep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit
Catalog #
19765
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (10)

Mouse Cell Isolation
Brochure
Mouse Cell Isolation
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Antigen Processing and Presentation
Wallchart
Antigen Processing and Presentation
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Streamline Your Mouse T Cell Research from Isolation to Expansion
30:18
Webinar
Streamline Your Mouse T Cell Research from Isolation to Expansion
Immunomagnetic Cell Isolation of Highly Purified Mouse Naïve CD4+ T Cells in 15 Minutes
Scientific Poster
Immunomagnetic Cell Isolation of Highly Purified Mouse Naïve CD4+ T Cells in 15 Minutes
Cell Enrichment of Mouse Naïve CD4+ T Cells from Spleen
Scientific Poster
Cell Enrichment of Mouse Naïve CD4+ T Cells from Spleen

常见问题

Can EasySep™ Streptavidin RapidSpheres™ be used for either positive or negative selection?

Currently, EasySep™ Streptavidin RapidSphere™ kits are only available for negative selection and work by targeting and removing unwanted cells.

How does the separation work?

Streptavidin RapidSphere™ magnetic particles are crosslinked to unwanted cells using biotinylated antibodies. When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a new tube.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ Streptavidin RapidSphere™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Are cells isolated using EasySep™ RapidSphere™ products FACS-compatible?

Yes. Desired cells are unlabeled and ready to use in downstream applications, such as FACS analysis.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

文献 (11)

A Bcl6 Intronic Element Regulates T Follicular Helper Cell Differentiation. C.-Y. Lai et al. Journal of immunology (Baltimore, Md. : 1950) 2022 sep

Abstract

In response to an intracellular infectious agent, the immune system produces a specific cellular response as well as a T cell-dependent Ab response. Precursor T cells differentiate into effector T cells, including Th1 cells, and T follicular helper (TFH) cells. The latter cooperate with B cells to form germinal centers and induce the formation of Ab-forming plasmacytes. One major focal point for control of T cell differentiation is the transcription factor BCL6. In this study, we demonstrated that the Bcl6 gene is regulated by FOXO1-binding, cis-acting sequences located in a highly conserved region of the first Bcl6 intron. In both mouse and human T cells, deletion of the tandem FOXO1 binding sites increased the expression of BCL6 and enhanced the proportion of TFH cells. These results reveal a fundamental control point for cellular versus humoral immunity.
View publication
pH sensing controls tissue inflammation by modulating cellular metabolism and endo-lysosomal function of immune cells. X. Chen et al. Nature immunology 2022 jul

Abstract

Extracellular acidification occurs in inflamed tissue and the tumor microenvironment; however, a systematic study on how pH sensing contributes to tissue homeostasis is lacking. In the present study, we examine cell type-specific roles of the pH sensor G protein-coupled receptor 65 (GPR65) and its inflammatory disease-associated Ile231Leu-coding variant in inflammation control. GPR65 Ile231Leu knock-in mice are highly susceptible to both bacterial infection-induced and T cell-driven colitis. Mechanistically, GPR65 Ile231Leu elicits a cytokine imbalance through impaired helper type 17 T cell (TH17 cell) and TH22 cell differentiation and interleukin (IL)-22 production in association with altered cellular metabolism controlled through the cAMP-CREB-DGAT1 axis. In dendritic cells, GPR65 Ile231Leu elevates IL-12 and IL-23 release at acidic pH and alters endo-lysosomal fusion and degradation capacity, resulting in enhanced antigen presentation. The present study highlights GPR65 Ile231Leu as a multistep risk factor in intestinal inflammation and illuminates a mechanism by which pH sensing controls inflammatory circuits and tissue homeostasis.
View publication
Mannan-Binding Lectin Promotes Murine Graft-versus-Host Disease by Amplifying Lipopolysaccharide-Initiated Inflammation. D. Heja et al. Transplantation and cellular therapy 2022 aug

Abstract

Conditioning regimens used for hematopoietic stem cell transplantation (HCT) can escalate the severity of acute T cell-mediated graft-versus-host disease (GVHD) by disrupting gastrointestinal integrity and initiating lipopolysaccharide (LPS)-dependent innate immune cell activation. Activation of the complement cascade has been associated with murine GVHD, and previous work has shown that alternative pathway complement activation can amplify T cell immunity. Whether and how mannan-binding lectin (MBL), a component of the complement system that binds mannose as well as oligosaccharide components of LPS and lipoteichoic acid, affects GVHD is unknown. In this study, we tested the hypothesis that MBL modulates murine GVHD and examined the mechanisms by which it does so. We adoptively transferred C3.SW bone marrow (BM) cells ± T cells into irradiated wild type (WT) or MBL-deficient C57Bl/6 (B6) recipients with or without inhibiting MBL-initiated complement activation using C1-esterase inhibitor (C1-INH). We analyzed the clinical severity of disease expression and analyzed intestinal gene and cell infiltration. In vitro studies assessed MBL expression on antigen-presenting cells (APCs) and compared LPS-induced responses of WT and MBL-deficient APCs. MBL-deficient recipients of donor BM ± T cells exhibited significantly less weight loss over the first 2 weeks post-transplantation weeks compared with B6 controls (P < .05), with similar donor engraftment in the 2 groups. In recipients of C3.SW BM + T cells, the clinical expression of GVHD was less severe (P < .05) and overall survival was better (P < .05) in MBL-deficient mice compared with WT mice. On day-7 post-transplantation, analyses showed that the MBL-deficient recipients exhibited less intestinal IL1b, IL17, and IL12 p40 gene expression (P < .05 for each) and fewer infiltrating intestinal CD11c+, CD11b+, and F4/80+ cells and TCR$\beta$+, CD4+, CD4+IL17+, and CD8+ T cells (P < .05 for each). Ovalbumin or allogeneic cell immunizations induced equivalent T cell responses in MBL-deficient and WT mice, demonstrating that MBL-deficiency does not directly impact T cell immunity in the absence of irradiation conditioning. Administration of C1-INH did not alter the clinical expression of GVHD in preconditioned WT B6 recipients, suggesting that MBL amplifies clinical expression of GVHD via a complement-independent mechanism. WT, but not MBL-deficient, APCs express MBL on their surfaces. LPS-stimulated APCs from MBL-deficient mice produced less proinflammatory cytokines (P < .05) and induced weaker alloreactive T cell responses (P < .05) compared with WT APCs. Together, our data show that MBL modulates murine GVHD, likely by amplifying complement-independent, LPS-initiated gastrointestinal inflammation. The results suggest that devising strategies to block LPS/MBL ligation on APCs has the potential to reduce the clinical expression of GVHD.
View publication
View All Publications

更多信息

更多信息
种属 Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 Other, Spleen
Selection Method Negative
标记抗体
  1. 抗小鼠CD62L（L-选择素）抗体，克隆MEL-14
    抗小鼠CD62L（L-选择素）抗体，克隆MEL-14

    抗小鼠CD62L（L-选择素）的大鼠单克隆IgG2a抗体

  2. 抗小鼠CD4抗体，克隆RM4-5
    抗小鼠CD4抗体，克隆RM4-5

    大鼠单克隆IgG2a抗体，抗小鼠CD4

质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
Copyright © 2025 by STEMCELL Technologies. All rights reserved.