抗小鼠CD4抗体，克隆RM4-5

大鼠单克隆IgG2a抗体，抗小鼠CD4

率先评论此产品

产品号 #（选择产品）

产品号 #60017_C

大鼠单克隆IgG2a抗体，抗小鼠CD4

跳到结尾的图片库

跳转到图像库的开头

总览

实验数据

产品说明书及文档

应用领域

总览

RM4-5抗体识别小鼠CD4，CD4是一种~59 kDa的单链1型跨膜糖蛋白，是免疫球蛋白（Ig）超家族的成员。CD4包含4个细胞外Ig样结构域。CD4在大多数胸腺细胞和一些T细胞亚群（辅助/诱导T细胞）中表达水平相对较高，而在树突状细胞中表达水平较低。在小鼠中，单核细胞/巨噬细胞不表达CD4。CD4结合MHC II的非多态性区域，并通过增强TCR与MHC II-抗原复合物之间结合的亲和力，在MHC II限制性抗原识别中作为T细胞受体（TCR）的共受体。CD4还通过胞内结构域与细胞质酪氨酸激酶（如Lck）的相互作用，放大从TCR到胞质的信号。RM4-5抗体与CD4的结合可抑制体外的配体结合；RM4-5抗体的结合可被抗体克隆GK1.5阻断。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠CD4+ T细胞分选试剂盒（产品号 #19852）和EasySep™小鼠CD25调节性T细胞正选试剂盒（产品号 #18782）分选的细胞纯度。

亚型
一抗

靶抗原
CD4

别名
L3T4，T4

活性物种
小鼠

偶联
Alexa Fluor 488，APC，Biotin 或生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的

宿主物种
大鼠

细胞类型
T 细胞，T 细胞，CD4+，T 细胞，调节性细胞

种属
小鼠

应用
细胞分选，CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹

研究领域
免疫

克隆
RM4-5

基因编号
12504

同种型
IgG2a，kappa

查看更多显示更少

实验数据

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, Alexa Fluor® 488 (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (Catalog #60076AD; solid line histogram).

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE; solid line histogram).

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ; solid line histogram).

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, Biotin followed by streptavidin (SAV) APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT) followed by SAV APC (solid line histogram).

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, FITC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI; solid line histogram).

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).