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抗小鼠CD25抗体，克隆PC61.5

大鼠单克隆IgG1抗体，抗小鼠CD25
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产品号 #（选择产品）

产品号 #60009_C

大鼠单克隆IgG1抗体，抗小鼠CD25

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

PC61.5抗体识别小鼠CD25（低亲和力白细胞介素-2受体α链，IL-2Rα），CD25是一种~55 kDa的1型跨膜糖蛋白，在T细胞和B细胞祖细胞、活化的（非静息）T细胞和B细胞，调节性T细胞（Treg）细胞，树突状细胞以及活化的单核细胞和巨噬细胞上表达。CD25、CD4和FOXP3的共表达被认为是Treg细胞的表型特征。CD25本身对其IL-2配体具有低亲和力，但与CD122 （IL-2Rβ）和CD132 （IL-2Rγ）结合形成高亲和力的IL-2R受体。CD25是受体聚合和诱导淋巴细胞活化和增殖相关信号通路所必需的。PC61.5抗体结合可抑制IL-2与低亲和力和高亲和力受体的结合，从而可能抑制IL-2依赖性的增殖。PC61.5识别的表位不同于IL-2结合位点，也不同于抗体7D4和3C7识别的表位。

亚型
一抗
 
靶抗原
CD25
 
别名
IL-2受体亚基α，IL-2Ra，IL-2Ralpha，IL-2-RA，白细胞介素-2受体亚基α，Ly-43，p55，Tac
 
活性物种
小鼠
 
偶联
APC，Biotin 或 生物素，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
大鼠
 
细胞类型
T 细胞，T 细胞，调节性细胞
 
种属
小鼠
 
应用
删除，流式细胞术，功能学筛选，免疫组化，免疫沉淀，中和及阻断
 
研究领域
免疫
 
克隆
PC61.5
 
基因编号
16184
 
同种型
IgG1，lambda
 

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实验数据

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071 (Catalog #60075), followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled withRat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC (Catalog #60075AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PE and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE (Catalog #60075PE) and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Figure 4. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PerCP-Cy5.5 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with a rat IgG1 isotype control antibody, PerCP-Cy5.5 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a biotinylated rat IgG1 isotype control antibody followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, APC
Catalog #
60009AZ, 60009AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin
Catalog #
60009BT.2, 60009BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, PE
Catalog #
60009PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, PerCP-Cy5.5
Catalog #
60009PS, 60009PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5
Catalog #
60009
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, APC
Catalog #
60009AZ, 60009AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin
Catalog #
60009BT.2, 60009BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, PE
Catalog #
60009PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, PerCP-Cy5.5
Catalog #
60009PS, 60009PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5
Catalog #
60009
Lot #
All
Language
English

相关材料与文献

技术资料 (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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更多信息
种属 Mouse
克隆 PC61.5
Gene Id 16184
Alternative Names IL-2 receptor subunit alpha, IL-2Ra, IL-2Ralpha, IL-2-RA, Interleukin-2 receptor subunit alpha, Ly-43, p55, Tac
同种型/isotype IgG1, lambda
法律声明:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

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