抗小鼠CD25抗体，克隆3C7

大鼠单克隆IgG2b抗体，抗小鼠CD25

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产品号 #（选择产品）

产品号 #60010_C

大鼠单克隆IgG2b抗体，抗小鼠CD25，未偶联

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总览

实验数据

产品说明书及文档

应用领域

总览

3C7抗体识别小鼠CD25（白细胞介素-2受体α链；IL-2Rα），CD25是一种~55 kDa的1型跨膜糖蛋白，在T细胞和B细胞祖细胞、活化的成熟的B细胞和T细胞表面表达。CD25已被用作鉴定小鼠CD4+/FoxP3+调节性T细胞的标志物。CD25的突变导致严重的免疫缺陷，强调了该蛋白在淋巴细胞的分化、激活和增殖以及维持自身耐受性方面所起的重要作用。CD25本身对其IL-2配体具有低亲和力，但可与CD122 （IL-2Rβ）和CD132 （IL-2Rγ）形成二聚体，并共同结合形成IL-2R受体，IL-2R受体以高亲和力结合IL-2。CD25的作用是增加IL-2结合的特异性和亲和力，是受体聚合和通过复合物的信号转导所必需的。3C7抗体的结合可抑制IL-2与游离CD25和IL- 2R受体的结合。该结合表位不同于抗CD25抗体克隆7D4和PC61识别的表位。该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠T细胞富集试剂盒（产品号 #19751））分选的细胞纯度。

亚型
一抗

靶抗原
CD25

别名
IL-2Ralpha，Ly-43，p55，Tac

活性物种
小鼠

偶联
FITC，PE，未偶联的

宿主物种
大鼠

细胞类型
T 细胞，T 细胞，调节性细胞

种属
小鼠

应用
细胞分选，CyTOF，流式细胞术，功能学筛选，免疫组化

研究领域
免疫

克隆
3C7

基因编号
16184

同种型
IgG2b，kappa

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实验数据

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on CD3e+ cells) labeled with Anti-Mouse CD25 Antibody, Clone 3C7, followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD4, Clone RM4-5, PE (Catalog #60017PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on CD3e+ cells) labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD4, Clone RM4-5, PE.

Figure 2. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD25 Antibody, Clone 3C7, FITC and anti-mouse CD3 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa FITC isotype control antibody and anti-mouse CD3 APC.
(C) Flow cytometry analysis of mouse T cells activated with antibodies against CD3 and CD28. C57BL/6 mouse splenocytes were processed with the EasySep™ Mouse T Cell Enrichment Kit and the enriched T cells were cultured in the absence (Unstimulated) or presence (Stimulated) of Anti-Mouse CD28 and plate-bound Anti-Mouse CD3e for 48 hours, then labeled with Anti-Mouse CD25 Antibody, Clone 3C7, FITC and analyzed for CD25 expression. Upregulation of CD25 following stimulation with CD3 and CD28 is apparent (Stimulated). Labeling of C57BL/6 mouse splenocytes with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown (open histogram).

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD25 Antibody, Clone 3C7, PE and anti-mouse CD3 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa PE isotype control antibody and anti-mouse CD3 APC.
(C) Flow cytometry analysis of mouse T cells activated with antibodies against CD3 and CD28. C57BL/6 mouse splenocytes were processed with the EasySep™ Mouse T Cell Enrichment Kit (Catalog #19751) and the enriched T cells were cultured in the absence (Unstimulated) or presence (Stimulated) of Anti-Mouse CD28 and plate-bound Anti-Mouse CD3e for 48 hours, then labeled with Anti-Mouse CD25 Antibody, Clone 3C7, PE and analyzed for CD25 expression. Upregulation of CD25 following stimulation with CD3 and CD28 is apparent (Stimulated). Labeling of C57BL/6 mouse splenocytes with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown (open histogram).