Rat IgG2a, kappa Isotype Control, Clone RTK2758

总览

RTK2758 抗体 (IgG2a, kappa) 适合在多种应用中用作同型对照抗体，以评估抗原特异性抗体的非特异性结合程度。理想情况下，同型对照应与所用特异性抗体具有相同的重链亚类（IgA、IgD、IgE、IgG 或 IgM）和轻链亚类（kappa 或 lambda）。如果使用偶联抗体，则应选择与相同分子（例如荧光染料）偶联的同型对照。使用合适的同型对照有助于确认抗原特异性抗体的特异性，并指示可能由与 Fc 受体或其他细胞成分结合引起的非特异性结合。

该抗体克隆已验证可用作同型对照抗体，用于评估流式细胞术和免疫荧光显微镜应用（表面和细胞内染色）等应用中的细胞非特异性结合。

分类
同型对照抗体

靶抗原
IgG2a 同型对照

别名
不适用

偶联
Alexa Fluor 488，APC，生物素，FITC，PE，未偶联

宿主物种
大鼠

细胞类型
其它细胞系

种属
人，小鼠，非人灵长类，其它细胞系，大鼠

应用
流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹

研究领域
免疫

克隆
RTK2758

Gene ID
不适用

Isotype
IgG2a, κ轻链

查看更多显示更少

实验数据

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, Catalog #60023) followed by a mouse anti-rat IgG2a antibody, FITC.

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, Alexa Fluor® 488, Catalog #60023AD).

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, APC, Catalog #60023AZ).

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, Biotin, Catalog #60023BT) followed by SAV APC.

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, FITC, Catalog #60023FI).

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, PE, Catalog #60023PE).