大鼠 IgM，kappa 同型对照抗体（克隆 RTK2118）

大鼠单克隆IgM， kappa同型对照抗体

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产品号 #（选择产品）

产品号 #60074_C

大鼠单克隆IgM， kappa同型对照抗体

总览

实验数据

产品说明书及文档

应用领域

总览

RTK2118 抗体 (IgM, kappa) 适合在多种应用中用作同型对照抗体，以评估抗原特异性抗体的非特异性结合程度。理想情况下，同型对照应与所用特异性抗体具有相同的重链亚类（IgA、IgD、IgE、IgG 或 IgM）和轻链亚类（kappa 或 lambda）。如果使用偶联抗体，则应选择与相同分子（例如荧光染料）偶联的同型对照。使用合适的同型对照有助于确认抗原特异性抗体的特异性，并指示可能由与 Fc 受体或其他细胞成分结合引起的非特异性结合。

该抗体克隆已验证可用作同型对照抗体，用于评估流式细胞术和免疫荧光显微镜应用（表面和细胞内染色）等应用中的细胞非特异性结合。

分类
同型对照抗体

靶抗原
IgM 同型对照

别名
不适用

偶联
Alexa Fluor 488，APC，生物素，FITC，PE，未偶联

宿主物种
大鼠

细胞类型
其它细胞系

种属
人，小鼠，非人灵长类，其它细胞系，大鼠

应用
流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹

研究领域
免疫

克隆
RTK2118

Gene ID
不适用

Isotype
IgM, κ

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实验数据

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, followed by a mouse anti-rat IgM antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5 (Catalog #60020), followed by a mouse anti-rat IgM antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE. (C) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631 (Catalog #60061), followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118 followed by goat anti-mouse IgG, FITC.

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, Alexa Fluor® 488 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, Alexa Fluor® 488 (Catalog #60020AD) and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, APC (Catalog #60020AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, Biotin (Catalog #60020BT) followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE. (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, FITC (Catalog #60020FI) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, PE (Catalog #60020PE) and Anti-Mouse CD45 Antibody, Clone 30-F11, APC. (C) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631, PE (Catalog #60061PE). Inset shows cells labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118.