小鼠IgM，kappa 同型对照抗体，克隆 MM-30

小鼠单克隆IgM， kappa同型对照抗体

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产品号 #（选择产品）

产品号 #60069_C

小鼠单克隆IgM， kappa同型对照抗体

总览

实验数据

产品说明书及文档

应用领域

总览

MM-30抗体（IgM, kappa）适合在多种应用中用作同型匹配的对照抗体，以评估抗原特异性抗体的非特异性结合程度。理想情况下，同型对照应具有与所用特异性抗体相同的重链亚类（IgA、IgD、IgE、IgG 或 IgM）和轻链亚类（kappa或lambda）。如果使用偶联抗体，则应选择与相同分子（如荧光染料）偶联的同型对照。使用合适的同型对照有助于确认抗原特异性抗体的特异性以及可能由与 Fc 受体或其他细胞成分结合引起的非特异性结合。据报道，MM-30 抗体可识别海兔血蓝蛋白。

该抗体克隆已被验证可用作同种型对照抗体，用于评估流式细胞术和免疫荧光显微镜应用（表面和细胞内染色）等应用中与细胞的非特异性结合。

分类
同型对照抗体

靶抗原
IgM 同型对照

别名
不适用

偶联
Alexa Fluor 488，PE，未偶联

宿主物种
小鼠

细胞类型
其它细胞系

种属
人，小鼠，非人灵长类，其它细胞系，大鼠

应用
流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹

研究领域
免疫

克隆
MM-30

Gene ID
不适用

Isotype
IgM, κ

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实验数据

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human H1 embryonic stem (ES) cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC (solid line histogram), or a mouse IgM, kappa positive control antibody (Anti-Human TRA160 Antibody, Clone TRA-1-60R; Catalog #60064) followed by goat anti-mouse IgG, FITC (filled histogram). (B) Human H1 ES cells were cultured in mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgM, kappa positive control antibody (Anti-Human TRA-1-81 Antibody, Clone TRA-1-81; Catalog #60065).

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human H1 embryonic stem (ES) cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488 (solid line histogram), or a mouse IgM, kappa positive control antibody (Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, Alexa Fluor® 488; Catalog #60064AD) (filled histogram).

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of human H1 embryonic stem (ES) cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (solid line histogram), or a mouse IgM, kappa positive control antibody (Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, PE; Catalog #60064PE) (filled histogram).