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ImmunoCult™小鼠T细胞激活试剂盒

小鼠T细胞激活和扩增试剂
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产品号 #（选择产品）

产品号 #100-1572_C

小鼠T细胞激活和扩增试剂

产品优势

  • 只需单个试剂盒即可高效地激活小鼠T细胞，并且可灵活地选择使用CD3/CD28或CD3/CD28/CD2进行激活。
  • 无需磁珠、饲养层细胞或抗原即可实现最佳激活。
  • 温和，可维持功能性T细胞的高存活率。
  • 高度稳定、过滤灭菌的可溶性试剂， 与常规T细胞生长培养基兼容。

产品组分包括

  • ImmunoCult™小鼠T细胞激活剂成分A， 1.0 mL
  • ImmunoCult™小鼠T细胞激活剂B组分，1.0 mL
  • ImmunoCult™小鼠T细胞激活剂C， 1.0 mL
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

ImmunoCult™小鼠T细胞激活试剂盒可温和地激活T细胞，从而保持T细胞的高存活率，激活后的T细胞可以在常规小鼠T细胞生长培养基中进一步扩增。该试剂盒由结合并交联CD3和CD28细胞表面配体的抗体复合物组成，还可以靶向CD2，从而为T细胞培养和激活提供所需的初级和共刺激信号。

使用所有3种试剂盒成分（A、B和C）可以在2-3天内实现最佳的T细胞激活。组分A含有用于必要抗原和共刺激激活信号的抗小鼠CD3和CD28抗体。组分B含有用于选择性靶向的抗小鼠CD2抗体。组分C有助于交联组分A和B中的抗体。所有试剂均高度稳定、经过过滤灭菌且可溶，无需耗时的培养板包被步骤，同时允许无缝衔接至当前的工作流程中。

为了有效分离小鼠T细胞，ImmunoCult™小鼠T细胞激活剂试剂盒可以与EasySep™工作流程结合使用。它与EasySep™小鼠T细胞分选试剂盒（产品号 #19851）、EasySep™小鼠CD4+T细胞分选（产品号 #19852）、EasySep™小鼠CD8+T细胞分选试剂盒（产品号 #19853）、EasySep™小鼠Naive CD4+ T细胞分选试剂盒（产品号 #19765）、EasySep™ Release小鼠CD4+CD304+调节性T细胞分选试剂盒（产品号 #100-1570）和EasySepTM小鼠CD4正选试剂盒II（产品号 #18952；肺组织）等试剂盒兼容。

包含
抗小鼠CD3单特异性抗体 抗小鼠CD28单特异性抗体 抗小鼠CD2单特异性抗体 抗体交联复合物
 
分类
完整试剂盒
 
细胞类型
T 细胞，T 细胞，CD4+，T 细胞，CD8+，T 细胞，调节性细胞
 
种属
小鼠
 
应用
激活，细胞培养，扩增
 
品牌
ImmunoCult
 
研究领域
免疫，细胞治疗开发
 

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实验数据

Figure 1. ImmunoCult™ Mouse T Cell Activator Kit Supports High Viability of Activated T Cells

Figure 1. Activation of EasySep™-Isolated Mouse T Cells Stimulated with ImmunoCult™ Mouse T Cell Activator Kit

Mouse T cells were isolated using EasySep™ Mouse T Cell Isolation Kit, stimulated with ImmunoCult™ Mouse T Cell Activator Kit, and cultured in IMDM + FBS formulation. Following 3 days of culture, the mean ± SD frequency of CD25+ cells was 91.9 ± 5.1% (n = 11) or 99.9 ± 0.1% (n = 5), when stimulated with ImmunoCult™ Mouse CD3/CD28 T Cell Activator or ImmunoCult™ Mouse CD3/CD28/CD2 T Cell Activator, respectively. Stimulated mouse T cells maintained expression levels of CD25 throughout the 7-day culture period.

Figure 2. Robust Expansion of EasySep™-Isolated Mouse T Cells Can Be Achieved Following Stimulation with ImmunoCult™ Mouse T Cell Activator Kit

Figure 2. Robust Expansion of EasySep™-Isolated Mouse T Cells Can Be Achieved Following Stimulation with ImmunoCult™ Mouse T Cell Activator Kit

EasySep™-isolated mouse T cells were expanded with ImmunoCult™ Mouse T Cell Activator Kit in IMDM + FBS formulation over 7 days. The number of viable cells was assessed every 2 - 3 days, when fresh medium supplemented with IL-2 was added. No additional ImmunoCult™ Mouse T Cell Activator was added during the 7-day culture period. After 7 days in culture with ImmunoCult™ Mouse CD3/CD28 T Cell Activator or ImmunoCult™ Mouse CD3/CD28/CD2 T Cell Activator, stimulation resulted in a fold expansion of 23 ± 3.4 or 29.3 ± 4.8 (mean ± SEM, n = 6), respectively.

Figure 3. High Cell Proliferation is Observed in EasySep™-Isolated T cells After Stimulation with ImmunoCult™ Mouse T Cell Activator

Figure 3. High Cell Proliferation Is Observed in EasySep™-Isolated T cells After Stimulation with ImmunoCult™ Mouse T Cell Activator

EasySep™-isolated mouse T cells were labeled with CFDA-SE (Catalog #75003), stimulated with ImmunoCult™ Mouse T Cell Activator Kit, and cultured in IMDM + FBS formulation. On Day 3, cells were harvested, stained with anti-mouse CD4 and CD8a antibodies, then measured by flow cytometry. Shown are CFDA-SE-labeled mouse T cells, gated on viable (A) CD4+ or (B) CD8a+ cells, cultured with no activator (top panel), with ImmunoCult™ Mouse CD3/CD28 T Cell Activator (middle panel), or with ImmunoCult™ Mouse CD3/CD28/CD2 T Cell Activator (bottom panel). Due to cell proliferation, the intensity of CFDA-SE signal is reduced by 50% for each cell division.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Mouse T Cell Activator Kit
Catalog #
100-1572
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
ImmunoCult™ Mouse T Cell Activator Kit
Catalog #
100-1572
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
ImmunoCult™ Mouse T Cell Activator Kit
Catalog #
100-1572
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
ImmunoCult™ Mouse T Cell Activator Kit
Catalog #
100-1572
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (2)

Mouse T Cell Research
Brochure
Mouse T Cell Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research

更多信息

更多信息
种属 Mouse
Contains Anti-mouse CD3 monospecific antibody Anti-mouse CD28 monospecific antibody Anti-mouse CD2 monospecific antibody Antibody crosslinking complex
法律声明:

Copyright © 2024 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, EasySep, and ImmunoCult are trademarks of STEMCELL Technologies Canada Inc. All other trademarks are the property of their respective holders. While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information. 质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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