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EasySep™小鼠记忆CD4+ T细胞分选试剂盒

利用免疫磁珠负选技术，27.5分钟完成细胞分选
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产品号 #（选择产品）

产品号 #19767_C

利用免疫磁珠负选技术，27.5分钟完成细胞分选

产品优势

  • 易于操作、快速
  • 纯度高达95%
  • 无需分离柱
  • 获得的活细胞无标记

产品组分包括

  • EasySep™小鼠记忆CD4+ T细胞分选试剂盒（产品号#19767）
    • EasySep™小鼠记忆CD4+ T细胞分选抗体混合物，0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠，2 x 1 mL
    • EasySep™ 小鼠FcR阻断剂（产品号#18730），0.2 mL
  • RoboSep™小鼠记忆CD4+ T细胞分选试剂盒（产品号#19767RF）
    • EasySep™小鼠记忆CD4+ T细胞分选抗体混合物，0.5 mL
    • EasySep™Streptavidin RapidSpheres™ 50001磁珠, 2 x 1 mL
    • EasySep™小鼠FcR阻断剂（产品号#18730），0.2 mL
    • RoboSep™ 缓冲液（产品号#20104）
    • RoboSep™过滤吸头（产品号#20125）
main product photo
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasyEights™ EasySep™ Magnet
    EasyEights™ EasySep™ Magnet

    Multiple sample processing magnet for column-free immunomagnetic cell separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

悬液中分选全记忆CD4+T细胞。非目的细胞可通过针对非记忆CD4+T细胞表面标志物（（CD8、CD11b、CD11c、CD19、CD24、CD45R、CD45RB、CD49b、CD105及TCRγ/δ）的生物素化抗体和链霉亲和素包被的磁珠（RapidSpheres™）而被去除。

通过EasySep™磁极，将这些被磁珠标记的细胞分离，无需使用分离柱。目的细胞被简单地倾倒至新管中。

了解更多采用 RapidSphere™ 技术的下一代 EasySep™ 小鼠细胞分选试剂盒。

通过EasySep™磁极，将这些被磁珠标记的细胞分离，无需使用分离柱。目的细胞被简单地倾倒至新管中。

了解更多采用 RapidSphere™ 技术的下一代 EasySep™ 小鼠细胞分选试剂盒。

通过EasySep™磁极，将这些被磁珠标记的细胞分离，无需使用分离柱。目的细胞被简单地倾倒至新管中。

了解更多采用 RapidSphere™ 技术的下一代 EasySep™ 小鼠细胞分选试剂盒。

通过EasySep™磁极，将这些被磁珠标记的细胞分离，无需使用分离柱。目的细胞被简单地倾倒至新管中。

了解更多采用 RapidSphere™ 技术的下一代 EasySep™ 小鼠细胞分选试剂盒。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
T 细胞，T 细胞，CD4+
 
种属
小鼠
 
样本来源
其它细胞系，Spleen
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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实验数据

Typical EasySep™ Mouse Memory CD4+ T Cell Isolation Profile

Figure 1. Typical EasySep™ Mouse Memory CD4+ T Cell Isolation Profile

Starting with mouse splenocytes, the memory CD4+ T cell (CD4+CD62L high CD44 high/int central memory and CD4+CD62L-CD44 high/int effector memory) content of the isolated fraction typically ranges from 78 - 96%. In the example above, the final purities of the start and isolated fractions are 5.3% and 90.2%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
1000157900 or higher
Language
English
Document Type
Product Information Sheet 2
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
1000157899 or lower
Language
English
Document Type
Product Information Sheet 1
Product Name
EasySep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767
Lot #
1000157900 or higher
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767
Lot #
1000157899 or lower
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (9)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Mouse Cell Isolation
Brochure
Mouse Cell Isolation
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Antigen Processing and Presentation
Wallchart
Antigen Processing and Presentation
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Streamline Your Mouse T Cell Research from Isolation to Expansion
30:18
Webinar
Streamline Your Mouse T Cell Research from Isolation to Expansion

常见问题

Can EasySep™ Streptavidin RapidSpheres™ be used for either positive or negative selection?

Currently, EasySep™ Streptavidin RapidSphere™ kits are only available for negative selection and work by targeting and removing unwanted cells.

How does the separation work?

Streptavidin RapidSphere™ magnetic particles are crosslinked to unwanted cells using biotinylated antibodies. When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a new tube.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ Streptavidin RapidSphere™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Are cells isolated using EasySep™ RapidSphere™ products FACS-compatible?

Yes. Desired cells are unlabeled and ready to use in downstream applications, such as FACS analysis.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

Which cell separation kits are compatible with the EasyPlate™ EasySep™ magnet?

The EasyPlate™-compatible human EasySep™ kits are:
19051 (T Cells), 19052 (CD4 T cells), 19157 (Memory CD4 T Cells), 19053 (CD8 T Cells), 19054 (B Cells), 19254 (Naïve B cells), 19055 (NK Cells), 19058 (Monocytes without CD16 depletion), 19059 (Monocytes)

The EasyPlate™-compatible mouse EasySep™ kits are:
19751 (T Cells), 19752 (CD4 T cells), 19753 (CD8 T Cells), 19754 (B Cells), 19755 (NK Cells - please contact Tech Support)

文献 (1)

T cell-intrinsic role for Nod2 in protection against Th17-mediated uveitis. R. J. Napier et al. Nature communications 2020

Abstract

Mutations in nucleotide-binding oligomerization domain-containing protein 2 (NOD2) cause Blau syndrome, an inflammatory disorder characterized by uveitis. The antimicrobial functions of Nod2 are well-established, yet the cellular mechanisms by which dysregulated Nod2 causes uveitis remain unknown. Here, we report a non-conventional, T cell-intrinsic function for Nod2 in suppression of Th17 immunity and experimental uveitis. Reconstitution of lymphopenic hosts with Nod2-/- CD4+ T cells or retina-specific autoreactive CD4+ T cells lacking Nod2 reveals a T cell-autonomous, Rip2-independent mechanism for Nod2 in uveitis. In naive animals, Nod2 operates downstream of TCR ligation to suppress activation of memory CD4+ T cells that associate with an autoreactive-like profile involving IL-17 and Ccr7. Interestingly, CD4+ T cells from two Blau syndrome patients show elevated IL-17 and increased CCR7. Our data define Nod2 as a T cell-intrinsic rheostat of Th17 immunity, and open new avenues for T cell-based therapies for Nod2-associated disorders such as Blau syndrome.
View publication
View All Publications

更多信息

更多信息
种属 Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 Other, Spleen
Selection Method Negative
标记抗体
  1. 抗小鼠CD4抗体，克隆RM4-5
    抗小鼠CD4抗体，克隆RM4-5

    大鼠单克隆IgG2a抗体，抗小鼠CD4

  2. 抗小鼠CD44抗体，克隆IM7
    抗小鼠CD44抗体，克隆IM7

    抗人、鼠、恒河猴CD44（组织非特异性碱性磷酸酶）的大鼠单克隆IgG2b抗体

  3. 抗小鼠CD62L（L-选择素）抗体，克隆MEL-14
    抗小鼠CD62L（L-选择素）抗体，克隆MEL-14

    抗小鼠CD62L（L-选择素）的大鼠单克隆IgG2a抗体

质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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