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抗小鼠CD62L（L-选择素）抗体，克隆MEL-14

抗小鼠CD62L（L-选择素）的大鼠单克隆IgG2a抗体
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产品号 #60109_C

抗小鼠CD62L（L-选择素）的大鼠单克隆IgG2a抗体

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

MEL-14 抗体可特异性识别小鼠CD62L（L-选择素或LECAM-1），CD62L是一种分子量约为74-95 kDa的I型糖蛋白，在大多数白细胞表面表达。CD62L通过结合CD34、glyCAM-1、MAdCAM-1和PSGL-1等配体，介导白细胞-内皮细胞的相互作用。其作用是促进淋巴细胞在血管内皮的滚动迁移，调控淋巴细胞向淋巴结的归巢，介导淋巴细胞及中性粒细胞向炎症部位的定向迁移。小鼠CD62L在大多数胸腺细胞中表达，在免疫功能正常或分裂中的祖细胞亚群以及中性粒细胞、嗜酸性粒细胞、单核细胞以及B细胞、T细胞和NK细胞亚群中高表达。 MEL-14抗体可与其他细胞表面标志物抗体联合使用，以区分naïveT细胞、记忆T细胞和效应T细胞。MEL-14抗体已被证实能够阻止淋巴细胞向淋巴结迁移，并抑制白细胞滚动黏附过程。

亚型
一抗
 
靶抗原
CD62L (L-selectin)
 
别名
CD62配体，L-选择素，LAM-1，LECAM-1，Leu-8，白细胞粘附分子1，白细胞-内皮细胞粘附分子1，Ly-22，MEL-14，PLn归巢受体，PLNHR
 
活性物种
小鼠
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
大鼠
 
细胞类型
T 细胞
 
种属
小鼠
 
应用
CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀
 
研究领域
免疫
 
克隆
MEL-14
 
基因编号
20343
 
同种型
IgG2a，kappa
 

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实验数据

Flow Cytometry Analysis of C57BL/6 Mouse Splenocytes

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Alexa Fluor® 488 (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (Catalog #60076AD) (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, FITC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) (solid line histogram).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE) (solid line histogram). (B) Flow cytometry analysis of mouse splenocytes (gated on CD4+ cells) processed with EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit and labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE and an anti-mouse CD44 antibody, FITC. Labeling of the Start splenocytes prior to cell isolation is shown (C).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Alexa Fluor® 488
Catalog #
60109AD, 60109AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Biotin
Catalog #
60109BT.1, 60109BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, FITC
Catalog #
60109FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE
Catalog #
60109PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PerCP-Cy5.5
Catalog #
60109PS
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14
Catalog #
60109.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Alexa Fluor® 488
Catalog #
60109AD, 60109AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Biotin
Catalog #
60109BT.1, 60109BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, FITC
Catalog #
60109FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE
Catalog #
60109PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PerCP-Cy5.5
Catalog #
60109PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14
Catalog #
60109.1
Lot #
All
Language
English

应用领域

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Research Area
Workflow Stages

更多信息

更多信息
种属 Mouse
克隆 MEL-14
Gene Id 20343
Alternative Names CD62 ligand, L-selectin, LAM-1, LECAM-1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, Ly-22, MEL-14, Pln homing receptor, PLNHR
同种型/isotype IgG2a, kappa
法律声明:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

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产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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