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EasySep™小鼠Naïve CD8+ T细胞分选试剂盒

小鼠Naïve CD8+ T细胞的免疫磁珠负选
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产品号 #19858_C

小鼠Naïve CD8+ T细胞的免疫磁珠负选

产品优势

  • 快捷、操作简单
  • 纯度高达98%
  • 无需分离柱
  • 获得的活细胞无标记     

产品组分包括

  • EasySep™小鼠Naïve CD8+ T细胞分选试剂盒（产品号 #19858）
    • EasySep™小鼠Naïve CD8+ T细胞分选抗体混合物，0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠 ，1 mL
    • EasySep™小鼠FcR阻断剂（产品号 #18720），0.1 mL          
  • RoboSep™小鼠Naïve CD8+ T细胞分选试剂盒（产品号 #19858RF  ）
    • EasySep™小鼠Naïve CD8+ T细胞分选抗体混合物，0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠 ，1 mL
    • EasySep™小鼠FcR阻断剂（ 产品号 #18720），0.1 mL
    • RoboSep™缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）
main product photo
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™小鼠Naïve CD8+ T细胞分选试剂盒，可通过免疫磁珠负选从小鼠脾脏 或淋巴结样本的单细胞悬液中轻松高效地分离高纯度小鼠初始CD8+ T细胞（CD3+CD8+CD44-CD62L+）。EasySep™结合了单克隆抗体的特异性和无柱磁性系统的简便性，迄今已广泛应用于发表的研究中超过20年。

在此EasySep™负选过程中，表达以下标记的非目标细胞通过抗体复合物和磁珠标记被靶向去除：CD4、CD11b、CD11c、CD19、CD44、CD45R/B220、CD49b、TCRγ/δ和TER119。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的     管中即可。仅需17.5分钟的磁珠     分选后，获得的          的初始CD8+ T细胞即可用于流式细胞术、细胞培养及基于细胞的实验等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选  。探索更多优化您实验流程的产品，包括培养基、添加剂、抗体等。

在此EasySep™负选过程中，表达以下标记的非目标细胞通过抗体复合物和磁珠标记被靶向去除：CD4、CD11b、CD11c、CD19、CD44、CD45R/B220、CD49b、TCRγ/δ和TER119。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的     管中即可。仅需17.5分钟的磁珠     分选后，获得的          的初始CD8+ T细胞即可用于流式细胞术、细胞培养及基于细胞的实验等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选  。探索更多优化您实验流程的产品，包括培养基、添加剂、抗体等。

在此EasySep™负选过程中，表达以下标记的非目标细胞通过抗体复合物和磁珠标记被靶向去除：CD4、CD11b、CD11c、CD19、CD44、CD45R/B220、CD49b、TCRγ/δ和TER119。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的     管中即可。仅需17.5分钟的磁珠     分选后，获得的          的初始CD8+ T细胞即可用于流式细胞术、细胞培养及基于细胞的实验等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选  。探索更多优化您实验流程的产品，包括培养基、添加剂、抗体等。

在此EasySep™负选过程中，表达以下标记的非目标细胞通过抗体复合物和磁珠标记被靶向去除：CD4、CD11b、CD11c、CD19、CD44、CD45R/B220、CD49b、TCRγ/δ和TER119。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的     管中即可。仅需17.5分钟的磁珠     分选后，获得的          的初始CD8+ T细胞即可用于流式细胞术、细胞培养及基于细胞的实验等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选  。探索更多优化您实验流程的产品，包括培养基、添加剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog 18102) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
T 细胞，T 细胞，CD8+
 
种属
小鼠
 
样本来源
其它细胞系，Spleen
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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实验数据

Typical EasySep™ Mouse Naïve CD8+ T Cell Isolation Profile

Figure 1. Typical EasySep™ Mouse Naïve CD8+ T Cell Isolation Profile

Starting with splenocytes from an uninfected mouse, the naïve CD8+ T cell (CD3+CD8+CD44-CD62L+) content of the isolated fraction typically ranges from 92 - 98%. In the example above, the final purities of the start and isolated fractions are 11.5% and 96.4%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
RoboSep™ Mouse Naïve CD8+T Cell Isolation Kit
Catalog #
19858RF
Lot #
1000147185 or higher
Language
English
Document Type
Product Information Sheet 2
Product Name
RoboSep™ Mouse Naïve CD8+T Cell Isolation Kit
Catalog #
19858RF
Lot #
1000147184 or lower
Language
English
Document Type
Product Information Sheet 1
Product Name
EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit
Catalog #
19858
Lot #
1000147184 or lower
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit
Catalog #
19858
Lot #
1000147185 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse Naïve CD8+T Cell Isolation Kit
Catalog #
19858RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse Naïve CD8+T Cell Isolation Kit
Catalog #
19858RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse Naïve CD8+T Cell Isolation Kit
Catalog #
19858RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Mouse Naïve CD8+T Cell Isolation Kit
Catalog #
19858RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
RoboSep™ Mouse Naïve CD8+T Cell Isolation Kit
Catalog #
19858RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit
Catalog #
19858
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit
Catalog #
19858
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit
Catalog #
19858
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit
Catalog #
19858
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (9)

Mouse Cell Isolation
Brochure
Mouse Cell Isolation
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Antigen Processing and Presentation
Wallchart
Antigen Processing and Presentation
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Using Stable Isotope Tracing to Study CD8+ Immune Cell Dynamics In Vivo
34:58
Webinar
Using Stable Isotope Tracing to Study CD8+ Immune Cell Dynamics In Vivo
Streamline Your Mouse T Cell Research from Isolation to Expansion
30:18
Webinar
Streamline Your Mouse T Cell Research from Isolation to Expansion

常见问题

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

Which cell separation kits are compatible with the EasyPlate™ EasySep™ magnet?

The EasyPlate™-compatible human EasySep™ kits are:
19051 (T Cells), 19052 (CD4 T cells), 19157 (Memory CD4 T Cells), 19053 (CD8 T Cells), 19054 (B Cells), 19254 (Naïve B cells), 19055 (NK Cells), 19058 (Monocytes without CD16 depletion), 19059 (Monocytes)

The EasyPlate™-compatible mouse EasySep™ kits are:
19751 (T Cells), 19752 (CD4 T cells), 19753 (CD8 T Cells), 19754 (B Cells), 19755 (NK Cells - please contact Tech Support)

Can EasySep™ Streptavidin RapidSpheres™ be used for either positive or negative selection?

Currently, EasySep™ Streptavidin RapidSphere™ kits are only available for negative selection and work by targeting and removing unwanted cells.

How does the separation work?

Streptavidin RapidSphere™ magnetic particles are crosslinked to unwanted cells using biotinylated antibodies. When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a new tube.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ Streptavidin RapidSphere™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Are cells isolated using EasySep™ RapidSphere™ products FACS-compatible?

Yes. Desired cells are unlabeled and ready to use in downstream applications, such as FACS analysis.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

文献 (5)

A Bcl6 Intronic Element Regulates T Follicular Helper Cell Differentiation. C.-Y. Lai et al. Journal of immunology (Baltimore, Md. : 1950) 2022 sep

Abstract

In response to an intracellular infectious agent, the immune system produces a specific cellular response as well as a T cell-dependent Ab response. Precursor T cells differentiate into effector T cells, including Th1 cells, and T follicular helper (TFH) cells. The latter cooperate with B cells to form germinal centers and induce the formation of Ab-forming plasmacytes. One major focal point for control of T cell differentiation is the transcription factor BCL6. In this study, we demonstrated that the Bcl6 gene is regulated by FOXO1-binding, cis-acting sequences located in a highly conserved region of the first Bcl6 intron. In both mouse and human T cells, deletion of the tandem FOXO1 binding sites increased the expression of BCL6 and enhanced the proportion of TFH cells. These results reveal a fundamental control point for cellular versus humoral immunity.
View publication
Direct interaction of whole-inactivated influenza A and pneumococcal vaccines enhances influenza-specific immunity. S. C. David et al. Nature microbiology 2019 may

Abstract

The upper respiratory tract is continuously exposed to a vast array of potentially pathogenic viruses and bacteria. Influenza A virus (IAV) has particular synergism with the commensal bacterium Streptococcus pneumoniae in this niche, and co-infection exacerbates pathogenicity and causes significant mortality. However, it is not known whether this synergism is associated with a direct interaction between the two pathogens. We have previously reported that co-administration of a whole-inactivated IAV vaccine (gamma-Flu) with a whole-inactivated pneumococcal vaccine (gamma-PN) enhances pneumococcal-specific responses. In this study, we show that mucosal co-administration of gamma-Flu and gamma-PN similarly augments IAV-specific immunity, particularly tissue-resident memory cell responses in the lung. In addition, our in vitro analysis revealed that S. pneumoniae directly interacts with both gamma-Flu and with live IAV, facilitating increased uptake by macrophages as well as increased infection of epithelial cells by IAV. These observations provide an additional explanation for the synergistic pathogenicity of IAV and S. pneumoniae, as well as heralding the prospect of exploiting the phenomenon to develop better vaccine strategies for both pathogens.
View publication
Autocrine Type I IFN Signaling in Dendritic Cells Stimulated with Fungal β-Glucans or Lipopolysaccharide Promotes CD8 T Cell Activation. Hassanzadeh-Kiabi N et al. Journal of immunology (Baltimore, Md. : 1950) 2016 NOV

Abstract

Type I IFNs are key mediators of immune defense against viruses and bacteria. Type I IFNs were also previously implicated in protection against fungal infection, but their roles in antifungal immunity have not been thoroughly investigated. A recent study demonstrated that bacterial and fungal β-glucans stimulate IFN-β production by dendritic cells (DCs) following detection by the Dectin-1 receptor, but the effects of β-glucan-induced type I IFNs have not been defined. We investigated whether type I IFNs regulate CD8 T cell activation by fungal β-glucan particle-stimulated DCs. We demonstrate that β-glucan-stimulated DCs induce CD8 T cell proliferation, activation marker (CD44 and CD69) expression, and production of IFN-γ, IL-2, and granzyme B. Moreover, we show that type I IFNs support robust CD8 T cell activation (proliferation and IFN-γ and granzyme B production) by β-glucan-stimulated DCs in vitro and in vivo due to autocrine effects on the DCs. Specifically, type I IFNs promote Ag presentation on MHC I molecules, CD86 and CD40 expression, and the production of IL-12 p70, IL-2, IL-6, and TNF-α by β-glucan-stimulated DCs. We also demonstrate a role for autocrine type I IFN signaling in bacterial LPS-induced DC maturation, although, in the context of LPS stimulation, this mechanism is not so critical for CD8 T cell activation (promotes IFN-γ production but not proliferation or granzyme B production). This study provides insight into the mechanisms underlying CD8 T cell activation during infection, which may be useful in the rational design of vaccines directed against pathogens and tumors.
View publication
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更多信息

更多信息
种属 Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog 18102) • RoboSep™-S (Catalog #21000)
样本来源 Other, Spleen
Selection Method Negative
标记抗体
  1. 抗小鼠CD62L（L-选择素）抗体，克隆MEL-14
    抗小鼠CD62L（L-选择素）抗体，克隆MEL-14

    抗小鼠CD62L（L-选择素）的大鼠单克隆IgG2a抗体

  2. 抗鼠CD8a抗体，克隆53-6.7
    抗鼠CD8a抗体，克隆53-6.7

    抗小鼠、蟾蜍CD8a的大鼠单克隆IgG2a抗体

  3. 抗小鼠CD3e抗体，克隆145-2C11
    抗小鼠CD3e抗体，克隆145-2C11

    亚美尼亚仓鼠单克隆IgG1抗体，抗小鼠CD3e

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