抗鼠CD8a抗体，克隆53-6.7

抗小鼠、蟾蜍CD8a的大鼠单克隆IgG2a抗体

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产品号 #（选择产品）

产品号 #60023_C

抗小鼠、蟾蜍CD8a的大鼠单克隆IgG2a抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

53-6.7抗体可特异性识别小鼠CD8a，CD8a是一种32 - 34 kDa的I型跨膜糖蛋白，是CD8的一个亚基。CD8是一种二硫键结合的二聚体，可以是CD8a （α）和CD8b （β）亚基的异二聚体（即αβ）形式或同二聚体（αα）形式。CD8作为T细胞受体（TCR）的共受体，在T细胞活化过程中结合抗原递呈细胞递呈的MHC I类分子。它的作用是加强TCR和MHC I-抗原复合物之间的结合，并通过胞内结构域与胞质酪氨酸激酶（如Lck）的相互作用，放大从TCR到细胞质的信号。CD8a链与MHC I类分子的α -3结构域结合。CD8在大多数胸腺细胞和一部分成熟的外周血T细胞（毒性T细胞）中以αβ形式表达，在γδ T细胞（肠上皮内淋巴细胞和树突状细胞的亚群）中以ββ形式表达。研究发现，53-6.7抗体可阻断通过MHC I类抗原的递呈，并抑制IL-2依赖性T细胞应答。该抗体克隆已验证可用于EasySep™试剂盒分离的细胞的纯度评估，包括EasySep™小鼠CD8+ T细胞分选试剂盒（产品号 #19853）和EasySep™小鼠CD90.2正选试剂盒II（产品号 #18951）。

亚型
一抗

靶抗原
CD8a

别名
Ly-2，Lyt2，T8

活性物种
小鼠，Toad

偶联
Alexa Fluor 488，APC，Biotin 或生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的

宿主物种
大鼠

细胞类型
T 细胞，T 细胞，CD8+

种属
小鼠，其它细胞系

应用
细胞分选，CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀

研究领域
免疫

克隆
53-6.7

基因编号
12525

同种型
IgG2a，kappa

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实验数据

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (solid line histogram).

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).