EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #18103_C
无柱免疫磁性分选多样本处理磁铁
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What Our Scientist Says
Isolating cells from multiple samples doesn't have to be tedious and time-consuming. That's why we've designed this magnet that can isolate cells from multiple samples in as little as 20 minutes.
Jennifer KennettTechnical Scientist
总览
搭配使用EasyEights™EasySep™Magnet和选定的EasySep™试剂盒，可同时对多达16个样本进行轻松高效的磁性细胞分选。EasyEights™EasySep™Magnet处理的样本体积范围较大，能够处理小体积样品，少至0.1 x 10^8个细胞，到大体积样本，多达8.5 x 10^8个细胞。该磁铁设计用于在一侧容纳8个标准5毫升（12 x 75毫米）聚苯乙烯圆底管，另一侧容纳8个标准14毫升（17 x 95毫米）圆底或15毫升锥形管。
不确定应该选择哪种磁铁？访问我们的EasySep™细胞分离磁铁页面比较不同的选项，并为您的研究选择合适的磁铁。
了解更多关于免疫磁性分选的知识EasySep™技术作品。
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
细胞分选
品牌
EasySep
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (9)
常见问题
文献 (1)
Whole Transcriptome Analysis Reveals That Filifactor alocis Modulates TNF$\alpha$-Stimulated MAPK Activation in Human Neutrophils. Frontiers in immunology 2020
Abstract
Periodontitis is an irreversible, bacteria-induced, chronic inflammatory disease that compromises the integrity of tooth-supporting tissues and adversely affects systemic health. As the immune system's first line of defense against bacteria, neutrophils use their microbicidal functions in the oral cavity to protect the host against periodontal disease. However, periodontal pathogens have adapted to resist neutrophil microbicidal mechanisms while still propagating inflammation, which provides essential nutrients for the bacteria to proliferate and cause disease. Advances in sequencing technologies have recognized several newly appreciated bacteria associated with periodontal lesions such as the Gram-positive anaerobic rod, Filifactor alocis. With the discovery of these oral bacterial species, there is also a growing need to assess their pathogenic potential and determine their contribution to disease progression. Currently, few studies have addressed the pathogenic mechanisms used by oral bacteria to manipulate the neutrophil functional responses at the level of the transcriptome. Thus, this study aims to characterize the global changes at the gene expression level in human neutrophils during infection with F. alocis. Our results indicate that the challenge of human neutrophils with F. alocis results in the differential expression of genes involved in multiple neutrophil effector functions such as chemotaxis, cytokine and chemokine signaling pathways, and apoptosis. Moreover, F. alocis challenges affected the expression of components from the TNF and MAPK kinase signaling pathways. This resulted in transient, dampened p38 MAPK activation by secondary stimuli TNF$\alpha$ but not by fMLF. Functionally, the F. alocis-mediated inhibition of p38 activation by TNF$\alpha$ resulted in decreased cytokine production but had no effect on the priming of the respiratory burst response or the delay of apoptosis by TNF$\alpha$. Since the modulatory effect was characteristic of viable F. alocis only, we propose this as one of F. alocis' mechanisms to control neutrophils and their functional responses.
更多信息
| 种属 | Human, Mouse, Non-Human Primate, Other, Rat |
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