EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #18001_C
无柱式免疫磁性分选磁铁
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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
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Lymphoprep™Density gradient medium for the isolation of mononuclear cells
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CryoStor® CS10Animal component-free, defined cryopreservation medium with 10% DMSO
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EasySep™ BufferCell separation buffer
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SepMate™-50 (IVD)Tube for density gradient centrifugation for in vitro diagnostic (IVD) applications
总览
搭配使用“The Big Easy”EasySep™磁铁和EasySep™细胞分离试剂，轻松高效地进行磁性细胞分选。当处理大量细胞时，该磁铁也可用作RoboSep™仪器的组成部分。“The Big Easy”EasySep™Magnet内部腔体中产生高梯度磁场，该磁场强度足够，无需使用分离柱即可分离带有EasySep™磁性颗粒的细胞。“The Big Easy”EasySep™磁铁设计用于容纳标准14 mL （17 x 95 mm）聚苯乙烯管。
不确定应该选择哪种磁铁？访问我们的EasySep™细胞分离磁铁页面比较不同的选项，并为您的研究选择合适的磁铁。
了解更多关于免疫磁性分选的知识EasySep™技术作品。
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
细胞分选
品牌
EasySep
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (10)
常见问题
文献 (1)
Norovirus Changes Susceptibility to Type 1 Diabetes by Altering Intestinal Microbiota and Immune Cell Functions. Frontiers in immunology 2019
Abstract
Environmental factors contribute to Type 1 diabetes (T1D) susceptibility. The gut microbiome, which includes bacteria, viruses, and fungi, contributes to this environmental influence, and can induce immunological changes. The gut viral component of the microbiome, related to T1D has mostly focused on coxsackieviruses and rotavirus. The role of norovirus, another common enteric virus, in susceptibility to T1D was hitherto unknown. Norovirus is highly infectious and encountered by many children. We studied the mouse norovirus 4 (MNV4), related to human noroviruses, in the Non-obese diabetic (NOD) mouse model, to determine its role in influencing susceptibility to T1D. We infected MNV-free NOD mice with MNV4 by exposing the mice to MNV4-positive bedding from an endemically-infected mouse colony to mimic a natural infection. Control MNV-free NOD mice were exposed to MNV-free bedding from the same colony. Interestingly, MNV4 infection protected NOD mice from the development of T1D and was associated with an expansion of Tregs and reduced proinflammatory T cells. We also found MNV4 significantly modified the gut commensal bacteria composition, promoting increased $\alpha$-diversity and Firmicutes/Bacteroidetes ratio. To elucidate whether T1D protection was directly related to MNV4, or indirectly through modulating gut microbiota, we colonized germ-free (GF) NOD mice with the MNV4-containing or non-MNV4-containing viral filtrate, isolated from filtered fecal material. We found that MNV4 induced significant changes in mucosal immunity, including altered Tuft cell markers, cytokine secretion, antiviral immune signaling markers, and the concentration of mucosal antibodies. Systemically, MNV4-infection altered the immune cells including B cell subsets, macrophages and T cells, and especially induced an increase in Treg number and function. Furthermore, in vitro primary exposure of the norovirus filtrate to na{\{i}}ve splenocytes identified significant increases in the proportion of activated and CTLA4-expressing Tregs. Our data provide novel knowledge that norovirus can protect NOD mice from T1D development by inducing the expansion of Tregs and reducing inflammatory T cells. Our study also highlights the importance of distinguishing the mucosal immunity mediated by bacteria from that by enteric viruses."
更多信息
| 种属 | Human, Mouse, Non-Human Primate, Other, Rat |
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