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Easy50 EasySep™磁铁

无柱免疫磁性分选磁铁
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产品号 #（选择产品）

产品号 #18002_C

无柱式免疫磁性分选磁铁

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总览
产品说明书及文档
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相关材料与文献
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总览

搭配使用Easy 50 EasySep™磁铁和EasySep™细胞分选试剂，轻松高效地进行磁性细胞分选。Easy 50 EasySep™Magnet内部腔体中产生高梯度磁场，该磁场强度足够，无需使用分离柱即可分离带有EasySep™磁性颗粒的细胞。Easy 50 EasySep™磁铁设计用于容纳标准50 mL锥形管，使您能够在单次分选中处理多达4 x 109细胞

不确定应该选择哪种磁铁？访问我们的EasySep™细胞分离磁铁页面比较不同的选项，并为您的研究选择合适的磁铁。

了解更多关于免疫磁性分选的知识EasySep™技术作品。

种属
人，小鼠，非人灵长类，其它细胞系
 
应用
细胞分选
 
品牌
EasySep
 

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产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Easy 50 EasySep™ Magnet
Catalog #
18002
Lot #
All
Language
English

相关材料与文献

技术资料 (5)

How to Isolate Cells Directly from Whole Blood Using the Easy 50 EasySep™ Magnet: 30-Minute Protocol
5:17
Video
How to Isolate Cells Directly from Whole Blood Using the Easy 50 EasySep™ Magnet: 30-Minute Protoc...
The Easy 50 EasySep™ Magnet: Portable Large-Volume Cell Separation In 25 Minutes
3:04
Video
The Easy 50 EasySep™ Magnet: Portable Large-Volume Cell Separation In 25 Minutes
How to Isolate Human Regulatory T Cells (Tregs) Using the Easy 50 EasySep™ Magnet
36:41
Video
How to Isolate Human Regulatory T Cells (Tregs) Using the Easy 50 EasySep™ Magnet
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
Human Immune Cell Isolation Course
On-Demand Training
Human Immune Cell Isolation Course

常见问题

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

Which cell separation kits are compatible with the "Easy 50" EASYSEP™ magnet?

At present, the "Easy 50" EasySep™ magnet is only compatible with EasySep™ kits for human cell separation.

T Cells: 19051 (T Cells), 19052 (CD4 T cells), 19157 (Memory CD4 T Cells), 19053 (CD8 T Cells), 19159 (Memory CD8 T Cells - please contact Tech Support)

B Cells: 19054 (B Cells), 19254 (Naïve B cells)

Other Cell Types: 19055 (NK Cells), 19058 (Monocytes without CD16 depletion), 19059 (Monocytes), 19062 (Plasmacytoid DCs), 19251 (pan-DCs)

For HLA Analysis: 19951HLA (T Cells from whole blood), 19954HLA (B Cells from whole blood), 19961HLA (Total lymphocytes from whole blood)

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种属 Human, Mouse, Non-Human Primate, Other
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