NeuroFluor™NeuO

用于检测活神经元的透膜荧光探针

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产品号 #（选择产品）

产品号 #01801_C

用于检测活神经元的透膜荧光探针

产品优势

使选择性标记小鼠，大鼠，或人类神经元不固定
能否证实人多能干细胞衍生神经祖细胞的神经分化
能在其他脑细胞存在的情况下用来标记神经元吗
无毒，非永久性
简单快速的标记方案

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总览

实验数据

产品说明书及文档

应用领域

总览

NeuroFluor™NeuO是一种膜渗透荧光探针，可在其他脑细胞存在的情况下选择性标记原代和多能干细胞来源的神经元（J C Er等）。新化学，2015)。用NeuroFluor™NeuO标记的细胞可以使用荧光成像可视化。用这种探针标记是非永久性的；它可以洗掉，为下游应用提供未标记的活细胞。有关其他信息，请参见参考资料。

Contains
• 100 µM NeuO (CAS Number: 1616355-50-0, 1668597-38-3)

• DMSO

Cell Type
Neural Cells, PSC-Derived, Neurons

Species
Human, Mouse, Rat

Application
Characterization, Phenotyping

Brand
NeuroFluor

Area of Interest
Disease Modeling, Neuroscience, Stem Cell Biology

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实验数据

Figure 1. NeuroFluor™ NeuO Selectively Labels Primary E18 Rat Neurons

(A) Primary rat E18 cortical neurons were labeled with 0.25μM NeuroFluor™ NeuO (green) and incubated for 1 hour. Image was taken after 2 hours of incubation. (B) The same culture was later fixed and stained for β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Nuclei are counterstained with DAPI. Images were taken at 20X magnification.

Figure 2. NeuroFluor™ NeuO Selectively Labels hPSC-Derived Neurons

(A) The neuronal precursors generated from hPSC-derived (XCL-1) neural progenitor cells were cultured in STEMdiff™ Neuron Maturation Medium. After 18 days of culture, hPSC-derived neurons were labeled with NeuroFluor™ NeuO (green). Nuclei are counterstained with DAPI. (B) The same culture was later fixed and stained with β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Images were taken at 20X magnification.