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NeuroFluor™NeuO

用于检测活神经元的透膜荧光探针

产品号 #(选择产品)

产品号 #01801_C

用于检测活神经元的透膜荧光探针

产品优势

  • 使选择性标记小鼠,大鼠,或人类神经元不固定
  • 能否证实人多能干细胞衍生神经祖细胞的神经分化
  • 能在其他脑细胞存在的情况下用来标记神经元吗
  • 无毒,非永久性
  • 简单快速的标记方案

总览

NeuroFluor™NeuO是一种膜渗透荧光探针,可在其他脑细胞存在的情况下选择性标记原代和多能干细胞来源的神经元(J C Er等)。新化学,2015)。用NeuroFluor™NeuO标记的细胞可以使用荧光成像可视化。用这种探针标记是非永久性的;它可以洗掉,为下游应用提供未标记的活细胞。有关其他信息,请参见参考资料。

Contains
• 100 µM NeuO (CAS Number: 1616355-50-0, 1668597-38-3)

• DMSO
 
Cell Type
Neural Cells, PSC-Derived, Neurons
 
Species
Human, Mouse, Rat
 
Application
Characterization, Phenotyping
 
Brand
NeuroFluor
 
Area of Interest
Disease Modeling, Neuroscience, Stem Cell Biology
 

实验数据

Figure 1. NeuroFluor™ NeuO Selectively Labels Primary E18 Rat Neurons

(A) Primary rat E18 cortical neurons were labeled with 0.25μM NeuroFluor™ NeuO (green) and incubated for 1 hour. Image was taken after 2 hours of incubation. (B) The same culture was later fixed and stained for β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Nuclei are counterstained with DAPI. Images were taken at 20X magnification.

Figure 2. NeuroFluor™ NeuO Selectively Labels hPSC-Derived Neurons

(A) The neuronal precursors generated from hPSC-derived (XCL-1) neural progenitor cells were cultured in STEMdiff™ Neuron Maturation Medium. After 18 days of culture, hPSC-derived neurons were labeled with NeuroFluor™ NeuO (green). Nuclei are counterstained with DAPI. (B) The same culture was later fixed and stained with β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Images were taken at 20X magnification.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
01801
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
01801
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (2)

文献 (1)

Neuo: A fluorescent chemical probe for live neuron labeling Er JC et al. Angewandte Chemie - International Edition 2015 FEB

Abstract

To address existing limitations in live neuron imaging, we have developed NeuO, a novel cell-permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation in vivo and in vitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications.

更多信息

更多信息
种属 Human, Mouse, Rat
Contains • 100 µM NeuO (CAS Number: 1616355-50-0, 1668597-38-3) • DMSO
质量保证:

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