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STEMdiff™ 中脑神经元分化试剂盒

用于将人胚胎干细胞(ES)和诱导多能干细胞(iPS)来源的神经祖细胞分化为中脑模式的神经前体细胞的分化试剂盒

产品号 #(选择产品)

产品号 #100-0038_C

用于将人胚胎干细胞(ES)和诱导多能干细胞(iPS)来源的神经祖细胞分化为中脑模式的神经前体细胞的分化试剂盒

产品优势

  • 成分明确且无血清
  • 支持从 hPSC 衍生的神经元前体细胞高效生成中脑样功能神经元
  • 产生大量 TH 阳性神经元 (> 15%),可在培养中长期维持
  • 针对使用 STEMdiff™ SMADi 神经诱导试剂盒生成的神经元祖细胞的分化进行了优化
  • 能够从多个人类ES和iPS细胞系中重复生成中脑型神经元前体

产品组分包括

  • STEMdiff™ 中脑神经元分化基础培养基,80 mL
  • STEMdiff™ 中脑神经元分化补充剂,20 mL
Need a high-quality cell source? Use the hiPSC SCTi003-A (female) control line, manufactured with mTeSR™ Plus.
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总览

STEMdiff™ 中脑神经元分化试剂盒(目录号:100-0038)用于从人多能干细胞(hPSCs)经 STEMdiff™ SMADi 神经诱导试剂盒(目录号:08581)诱导得到的神经祖细胞(NPCs)中生成中脑神经前体细胞。之后,使用 STEMdiff™ 中脑成熟试剂盒(目录号:100-0041)进一步将中脑神经元前体细胞成熟为中脑神经元。该系列培养基将产生中脑神经元群(≥ 15% TH 阳性多巴胺能神经元;≥ 90% III 类 β 微管蛋白阳性神经元;< 10% GFAP 阳性星形胶质细胞)。使用这些产品衍生的细胞可作为构建人类神经发育和疾病模型建立、药物筛选、毒性测试和细胞疗法验证的多功能工具。

亚型
专用培养基
 
细胞类型
多巴胺能神经元,神经细胞,PSC衍生,神经干/祖细胞
 
种属

 
应用
细胞培养,分化
 
品牌
STEMdiff
 
研究领域
疾病建模,药物发现和毒理检测,神经科学
 
制剂类别
无血清
 

实验数据

Experimental Protocol for STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits (Embryoid Body Protocol)

Figure 1. Schematic for the Embryoid Body Protocol

Midbrain-type neural precursors can be generated in 18 - 19 days from hPSC-derived neural progenitor cells (NPCs) after selecting neural rosettes from replated embryoid bodies. For the maturation of precursors to midbrain-type neurons, including dopaminergic neurons, see the PIS.

Experimental Protocol for STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits (Monolayer Protocol)

Figure 2. Schematic for the Monolayer Protocol

Midbrain-type neural precursors can be generated from neural progenitor cell (NPC) monolayers derived from embryonic and induced pluripotent stem cells after three single-cell passages. For the maturation of precursors to midbrain-type neurons, including dopaminergic neurons, see the PIS.

Midbrain-Type Neurons Arise From Neural Progenitor Cells After Culture in STEMdiff™ SMADi Neural Induction Kit and STEMdiff™ Midbrain Culture System

Figure 3. Midbrain-Type Neurons Are Generated After Culture in STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits

NPCs generated from H1 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit embryoid body (EB) protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after hPSC-derived NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), with (D) more than 15% Tyrosine Hydroxylase-positive cells (green). (C) Nuclei are labeled with DAPI (blue).

Figure 4. Midbrain-Type Neural Precursor Cells Express Characteristic Markers After Culture in STEMdiff™ Midbrain Neuron Differentiation Kit

NPCs generated from STiPS-F016 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit monolayer protocol were differentiated to midbrain-type neural precursors using STEMdiff™ Midbrain Neuron Differentiation Kit for 7 days. (A) Midbrain-type neural precursor cell cultures contain a population of (A) FOXA2-expressing cells (red) and (B) NKX2.1-expressing cells (green). Merge image in (E) showing the bottom half of the same FOV includes DAPI-labeled nuclei (blue) and shows non-overlapping marker expression. The resulting neural precursor cultures also express (C) OTX2 (red) and are negative for (D) central nervous system NPC marker PAX6 (green). Merge image in (F) showing the bottom half of the same FOV includes nuclei that are labeled with DAPI (blue).

Figure 5. Midbrain-Type Neurons Express Dopamine Transporters (DAT) After Differentiation and Maturation in STEMdiff™ Midbrain Neuron Kits

NPCs generated from H9 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit monolayer protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), which (C) express DAT in blue, and (E) Tyrosine Hydroxylase-positive cells (green). (D) Nuclei are labeled with DAPI (white).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0038
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0038
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0038
Lot #
All
Language
English

应用领域

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更多信息

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种属 Human
配方类别 Serum-Free
质量保证:

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