STEMdiff™ 中脑神经元成熟试剂盒

用于从人ES和iPS细胞衍生的神经元前体细胞生成功能性中脑型神经元的成熟试剂盒

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产品号 #（选择产品）

产品号 #100-0041_C

用于从人ES和iPS细胞衍生的神经元前体细胞生成功能性中脑型神经元的成熟试剂盒

产品优势

成分明确且无血清
支持从 hPSC 衍生的神经元前体细胞高效生成中脑样功能神经元
产生大量TH阳性神经元 (>15%)，可在培养中长期维持
针对使用 STEMdiff™ 中脑神经元分化试剂盒生成的中脑神经元前体的成熟进行了优化
支持神经元活性，以获得生理相关结果
实现源自多个人ES和iPS细胞系的中脑型神经元前体的可重复成熟

产品组分包括

STEMdiff™ 中脑神经元成熟补充剂，25 mL
BrainPhys™ 神经元培养基，100 mL

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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

ACCUTASE™
Cell detachment solution
STEMdiff™ SMADi Neural Induction Kit
Serum-free medium kit for highly efficient SMAD inhibition-mediated neural induction of human ES and iPS cells
STEMdiff™ Midbrain Neuron Differentiation K...
Differentiation kit for the generation of midbrain-patterned neuronal precursors from human ES and iPS cell-derived neural progenitor cells
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

STEMdiff™ 中脑神经元成熟试剂盒（目录号 #100-0041）与 STEMdiff™ 中脑神经元分化试剂盒（目录号 #100-0038）配合使用，用于从人多能干细胞衍生的神经前体细胞中生成混合型中脑神经元群体。该培养基可生成以下中脑神经元群体（≥ 15% TH 阳性多巴胺能神经元；≥ 90% III 类 β 微管蛋白阳性神经元；< 10% GFAP 阳性星形胶质细胞）。通过这些产品获得的细胞是研究人类神经发育和疾病建模、药物筛选、毒性测试以及细胞治疗验证的多功能工具。

亚型
专用培养基

细胞类型
多巴胺能神经元，神经细胞，PSC衍生，神经元

种属
人

应用
细胞培养，鉴定，分化，功能学筛选，免疫细胞化学，表型鉴定

品牌
STEMdiff

研究领域
疾病建模，药物发现和毒理检测，神经科学

制剂类别
无血清

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实验数据

Experimental Protocol for STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits (Embryoid Body Protocol)

Figure 1. Schematic for the Embryoid Body Protocol

Midbrain-type neurons, including dopaminergic neurons, can be generated from neural precursors after 12 days in STEMdiff™ Midbrain Neuron Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS.

Experimental Protocol for STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits (Monolayer Protocol)

Figure 2. Schematic for the Monolayer Protocol

Midbrain-type neurons, including dopaminergic neurons, can be generated from neural precursors after 6 days in STEMdiff™ Midbrain Neuron Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS.

Midbrain-Type Neurons Arise From Neural Progenitor Cells After Culture in STEMdiff™ SMADi Neural Induction Kit and STEMdiff™ Midbrain Culture System

Figure 3. Midbrain-Type Neurons Are Generated After Culture in STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits

NPCs generated from H1 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit embryoid body (EB) protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after hPSC-derived NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), with (D) more than 15% Tyrosine Hydroxylase-positive cells (green). (C) Nuclei are labeled with DAPI (blue).

Figure 4. Midbrain-Type Neural Precursor Cells Express Characteristic Markers After Culture in STEMdiff™ Midbrain Neuron Differentiation Kit

NPCs generated from STiPS-F016 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit monolayer protocol were differentiated to midbrain-type neural precursors using STEMdiff™ Midbrain Neuron Differentiation Kit for 7 days. (A) Midbrain-type neural precursor cell cultures contain a population of (A) FOXA2-expressing cells (red) and (B) NKX2.1-expressing cells (green). Merge image in (E) showing the bottom half of the same FOV includes DAPI-labeled nuclei (blue) and shows non-overlapping marker expression. The resulting neural precursor cultures also express (C) OTX2 (red) and are negative for (D) central nervous system NPC marker PAX6 (green). Merge image in (F) showing the bottom half of the same FOV includes nuclei that are labeled with DAPI (blue).

Figure 5. Midbrain-Type Neurons Express Dopamine Transporters (DAT) After Differentiation and Maturation in STEMdiff™ Midbrain Neuron Kits

NPCs generated from H9 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit monolayer protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), which (C) express DAT in blue, and (E) Tyrosine Hydroxylase-positive cells (green). (D) Nuclei are labeled with DAPI (white).