产品号 #02693_C
人粒细胞扩增和分化的无血清培养补充物
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StemSpan™髓系扩增补充(100X)含有重组人细胞因子的组合,用于选择性地促进人髓系祖细胞的扩增和分化,从人脐带血(CB)或骨髓(BM)样本中分离的CD34+细胞产生大量CD15+粒细胞。
StemSpan™髓系扩增补剂可与以下任何StemSpan™培养基结合使用:
•StemSpan™SFEM(目录#09600)
StemSpan™SFEM II(目录#09605)
StemSpan™-XF(目录#100-0073)
StemSpan™-AOF(目录#100-0130)
当添加到无血清培养基中时,StemSpan™髓系扩增补充剂通常促进每个输入CD34+细胞在CD34+人CB细胞启动的14天液体培养中产生数千个CD15+粒细胞。请参阅data选项卡了解更多详细信息。
Contains
• Recombinant human stem cell factor (SCF)
• Recombinant human thrombopoietin (TPO)
• Recombinant human granulocyte colony-stimulating factor (G-CSF)
• Recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF)
Subtype
Supplements
Cell Type
Hematopoietic Stem and Progenitor Cells, Myeloid Cells
Species
Human
Application
Cell Culture, Differentiation, Expansion
Brand
StemSpan
Area of Interest
Stem Cell Biology, Transplantation Research
Formulation Category
Serum-Free
Figure 1. Production of CD13+ and CD15+ Myeloid Cells by the Expansion and Lineage-Specific Differentiation of Human BM-Derived Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement
Flow cytometry dot plots for 14-day cultures of human BM-derived CD34+ cells in StemSpan™ SFEM II containing Myeloid Expansion Supplement showing expression of the HSPC marker CD34, and pan-hematopoietic marker CD45 (A) before and (B) after 14 days of culture, and expression of myeloid markers CD13 and CD15 (C) after 14 days of culture. The frequency of CD34+ cells declined from 83% before culture to ~1% after 14 days. In parallel, myeloid CD13+CD15+ cells gradually accumulated from <10% on day 0 to 46% by day 14. The frequency of CD14+ cells after culture was low (typically <10%, see Table 1).
Table 1. Production of Myeloid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement
Number of total nucleated cells (TNCs) produced per input human CB-derived CD34+ cell from 14 different donors and percentage of cells positive for myeloid markers CD13, CD14 and CD15 produced after 14 days of culture in SFEM II containing Myeloid Expansion Supplement. Best expansion was obtained in SFEM II, but myeloid cell expansion may also be obtained in SFEM and ACF media.
*95% confidence limits (CL), the range within which 95% of the results will typically fall.
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种属 | Human |
---|---|
Contains | • Recombinant human stem cell factor (SCF) • Recombinant human thrombopoietin (TPO) • Recombinant human granulocyte colony-stimulating factor (G-CSF) • Recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF) |
配方类别 | Serum-Free |
长期培养人体细胞的培养基
用于培养和扩增造血细胞的无血清培养基
用于培养和扩增造血细胞的无血清培养基
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