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StemSpan™髓系扩增补品（100X）

人粒细胞扩增和分化的无血清培养补充物
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产品号 #（选择产品）

产品号 #02693_C

人粒细胞扩增和分化的无血清培养补充物

产品优势

  • 在14天的液体培养中，促进每个输入CD34+人CB细胞产生数千个CD15+粒细胞。
  • 作为100X浓缩物供应。在解冻和混合后，试管内容物可以直接添加到任何选择的造血细胞扩增培养基中。
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

  • 在14天的液体培养中，每个接种的CD34+人CB细胞可生成数千个CD15+粒细胞。
  • 以100X浓缩液规格供应。
  • 在解冻和混匀后，试剂可以直接添加到任何您使用的造血细胞扩增培养基中。

包含
• 重组人干细胞因子（SCF） • 重组人血小板生成素（TPO） • 重组人粒细胞集落刺激因子（G-CSF） • 重组人粒细胞/巨噬细胞集落刺激因子（GM-CSF）
 
分类
添加剂
 
细胞类型
造血干/祖细胞，髓系细胞
 
种属
人
 
应用
细胞培养，分化，扩增
 
品牌
StemSpan
 
研究领域
干细胞生物学，移植研究
 
制剂类别
无血清
 

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实验数据

Production of CD13+ and CD15+ Myeloid Cells by the Expansion and Lineage-Specific Differentiation of Human BM-Derived Cells Cultured in StemSpan™ SFEM II Containing Myeloid CD34+ Expansion Supplement

Figure 1. Production of CD13+ and CD15+ Myeloid Cells by the Expansion and Lineage-Specific Differentiation of Human BM-Derived Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement

Flow cytometry dot plots for 14-day cultures of human BM-derived CD34+ cells in StemSpan™ SFEM II containing Myeloid Expansion Supplement showing expression of the HSPC marker CD34, and pan-hematopoietic marker CD45 (A) before and (B) after 14 days of culture, and expression of myeloid markers CD13 and CD15 (C) after 14 days of culture. The frequency of CD34+ cells declined from 83% before culture to ~1% after 14 days. In parallel, myeloid CD13+CD15+ cells gradually accumulated from <10% on day 0 to 46% by day 14. The frequency of CD14+ cells after culture was low (typically <10%, see Table 1).

Table 1. Production of Myeloid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement

Production of Myeloid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement

Number of total nucleated cells (TNCs) produced per input human CB-derived CD34+ cell from 14 different donors and percentage of cells positive for myeloid markers CD13, CD14 and CD15 produced after 14 days of culture in SFEM II containing Myeloid Expansion Supplement. Best expansion was obtained in SFEM II, but myeloid cell expansion may also be obtained in SFEM and ACF media.
*95% confidence limits (CL), the range within which 95% of the results will typically fall.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
StemSpan™ Myeloid Expansion Supplement (100X)
Catalog #
02693
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
StemSpan™ Myeloid Expansion Supplement (100X)
Catalog #
02693
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

更多信息

更多信息
种属 Human
Contains • Recombinant human stem cell factor (SCF) • Recombinant human thrombopoietin (TPO) • Recombinant human granulocyte colony-stimulating factor (G-CSF) • Recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF)
配方类别 Serum-Free
质量保证:

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