EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #72202_C
Hedgehog 通路激活剂；激活 Smoothened (SMO)
若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系
总览
Purmorphamine 是一种三取代嘌呤衍生物，通过直接结合并激活 Hedgehog 受体 Smoothened (EC₅₀ = 1 µM) 来激活 Hedgehog 通路。（Sinha and Chen）
分化
·促进人多能干细胞向腹侧脊髓祖细胞和运动神经元分化（Hu and Zhang, Karumbayaram et al., Li et al.）。
·促进人和小鼠间充质细胞向成骨细胞分化（Beloti et al., Wu et al. 2002, Wu et al. 2004）。
·抑制人间充质细胞向脂肪细胞分化和成熟（Fontaine et al.）。
细胞类型
间充质干/祖细胞，神经细胞，PSC衍生，成骨细胞，多能干细胞
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
分化
研究领域
神经科学，干细胞生物学
CAS 编号
483367-10-8
化学式
C₃₁H₃₂N₆O₂
纯度
≥98%
通路
Hedgehog
靶点
SMO
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (4)
文献 (8)
Differentiation of spinal motor neurons from pluripotent human stem cells. Nature protocols 2009 JAN
Abstract
We have devised a reproducible protocol by which human embryonic stem cells (hESCs) or inducible pluripotent stem cells (iPSCs) are efficiently differentiated to functional spinal motor neurons. This protocol comprises four major steps. Pluripotent stem cells are induced to form neuroepithelial (NE) cells that form neural tube-like rosettes in the absence of morphogens in the first 2 weeks. The NE cells are then specified to OLIG2-expressing motoneuron progenitors in the presence of retinoic acid (RA) and sonic hedgehog (SHH) or purmorphamine in the next 2 weeks. These progenitor cells further generate post-mitotic, HB9-expressing motoneurons at the 5th week and mature to functional motor neurons thereafter. It typically takes 5 weeks to generate the post-mitotic motoneurons and 8-10 weeks for the production of functional mature motoneurons. In comparison with other methods, our protocol does not use feeder cells, has a minimum dependence on proteins (purmorphamine replacing SHH), has controllable adherent selection and is adaptable for scalable suspension culture.
Directed differentiation of human-induced pluripotent stem cells generates active motor neurons. Stem cells (Dayton, Ohio) 2009 APR
Abstract
The potential for directed differentiation of human-induced pluripotent stem (iPS) cells to functional postmitotic neuronal phenotypes is unknown. Following methods shown to be effective at generating motor neurons from human embryonic stem cells (hESCs), we found that once specified to a neural lineage, human iPS cells could be differentiated to form motor neurons with a similar efficiency as hESCs. Human iPS-derived cells appeared to follow a normal developmental progression associated with motor neuron formation and possessed prototypical electrophysiological properties. This is the first demonstration that human iPS-derived cells are able to generate electrically active motor neurons. These findings demonstrate the feasibility of using iPS-derived motor neuron progenitors and motor neurons in regenerative medicine applications and in vitro modeling of motor neuron diseases.
Directed differentiation of ventral spinal progenitors and motor neurons from human embryonic stem cells by small molecules. Stem cells (Dayton, Ohio) 2008 APR
Abstract
Specification of distinct cell types from human embryonic stem cells (hESCs) is key to the potential application of these naïve pluripotent cells in regenerative medicine. Determination of the nontarget differentiated populations, which is lacking in the field, is also crucial. Here, we show an efficient differentiation of motor neurons ( approximately 50%) by a simple sequential application of retinoid acid and sonic hedgehog (SHH) in a chemically defined suspension culture. We also discovered that purmorphamine, a small molecule that activates the SHH pathway, could replace SHH for the generation of motor neurons. Immunocytochemical characterization indicated that cells differentiated from hESCs were nearly completely restricted to the ventral spinal progenitor fate (NKX2.2+, Irx3+, and Pax7-), with the exception of motor neurons (HB9+) and their progenitors (Olig2+). Thus, the directed neural differentiation system with small molecules, even without further purification, will facilitate basic and translational studies using human motoneurons at a minimal cost.
更多信息
| 种属 | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Cas Number | 483367-10-8 |
| Chemical Formula | C₃₁H₃₂N₆O₂ |
| 纯度 | ≥ 98% |
| Target | SMO |
| Pathway | Hedgehog |
相关产品
-
STEMdiff™ 神经诱导培养基用于人 ES 和 iPS 细胞神经诱导的成分明确的无血清培养基
-
GSA 10Hedgehog通路激活剂；激活Smoothened (SMO)蛋白
-
SAGHedgehog通路激活剂；激活Smoothened（SMO）
