qPCR预混试剂盒

适用于探针法检测与微阵列分析

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产品号 #07516_C

适用于探针法检测与微阵列分析

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Nuclease-Free Water
DNase- and RNase-free water for molecular biology applications
Total RNA Purification Kit
For purification of RNA from cells
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

qPCR 预混液是一种2X浓缩溶液，针对基于TaqMan®探针的实时定量聚合酶链反应（qPCR）。它需要与基因特异性引物（扩增目标cDNA）和荧光标记探针（利用Taq DNA聚合酶的5 '核酸酶活性产生荧光信号）结合使用。荧光信号的积累速率用于定量cDNA，从而确定原始样本中mRNA的含量。qPCR预混液包括热启动DNA聚合酶，dNTPs, MgCl2，增强剂和稳定剂。qPCR预混液试剂盒还包括ROX参考染料作为单独的组分，使其兼容依赖或非依赖参考染料的qPCR系统。• 高效、灵敏且可重复 • 在标准或快速循环条件下均具有最佳性能 • 非常适合高通量应用和过夜实验 • 兼容各种 qPCR 仪器

细胞类型
脂肪细胞，软骨细胞，内胚层，PSC衍生，肝细胞，肠道细胞，间充质干/祖细胞，成骨细胞，胰腺细胞，多能干细胞

种属
人

应用
基因组编辑

研究领域
干细胞生物学

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实验数据

Figure 1. PCR Amplification Efficiency Remains Consistently High Under Standard or Fast Cycling Conditions

qPCR was performed with qPCR array plates, template, qPCR Master Mix and reference dye using a real-time PCR instrument. (A) The calculated PCR efficiency of 13 assays (n = 26) is shown run under fast cycling conditions using either diluted cDNA (50–0.016 ng) or 101 –107 copies of template. All assays exhibited 90–110% PCR efficiency with R2 >0.99. (B) At each concentration of cDNA (50 – 0.016 ng; 3 of 6 dilutions shown), the difference in Cq values determined using standard or fast cycling conditions was <1. Standard cycling: 3 min. 95°C; 49 x (15 sec. 95°C; 1 min. 60°). Fast cycling: 3 min. 95°C; 49 x (5 sec. 95°C; 30 sec. 60°).

Figure 2. Reproducible Amplification with qPCR Master Mix After Extended Pre-Heating

qPCR Master Mix was either heated at 55°C for 4 or 8 hours or not heated before use in qPCR assays with reference dye and varying amounts of cDNA (50 - 0.08 ng). An overlay of the amplification plots shows no effect on the amplification curves for unheated or heated qPCR Master Mix.