EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #15027_C
免疫密度负选试剂混合物
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Lymphoprep™Density gradient medium for the isolation of mononuclear cells
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Dulbecco's Phosphate Buffered Saline with 2% ...Cell culture buffer
总览
RosetteSep™人骨髓祖细胞预富集抗体混合物 通过负选从全骨髓去除谱系特异性细胞来 预富集祖细胞。四聚体抗体复合物可识别CD3,、CD11b 、 CD14 、 CD16 、 CD19 、 CD56 、 CD66b以及红细胞（RBC）上的糖蛋白A，从而靶向去除非目的细胞。使用密度梯度离心液如Lymphoprep™（产品号 #18060）离心后 ，非目的细胞会与红细胞一起沉淀。预富集的祖细胞为血浆和密度梯度离心液的交界界面中高度富集的细胞。
磁体兼容性
亚型
细胞分选试剂盒
细胞类型
造血干/祖细胞
种属
人
样本来源
Bone Marrow
筛选方法
Negative
应用
细胞分选
品牌
RosetteSep
研究领域
免疫，干细胞生物学
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
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相关材料与文献
技术资料 (4)
常见问题
文献 (2)
Growth of mesenchymal stem cells on electrospun type I collagen nanofibers. Stem cells (Dayton, Ohio) 2006 NOV
Abstract
We reconstituted type I collagen nanofibers prepared by electrospin technology and examined the morphology, growth, adhesion, cell motility, and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) on three nano-sized diameters (50-200, 200-500, and 500-1,000 nm). Results from scanning electron microscopy showed that cells on the nanofibers had a more polygonal and flattened cell morphology. MTS (3-[4,5-dimethythiazol-2-yl]-5-[3-carboxy-methoxyphenyl]-2-[4-sul-fophenyl]-2H-tetrazolium compound) assay demonstrated that the MSCs grown on 500-1,000-nm nanofibers had significantly higher cell viability than the tissue culture polystyrene control. A decreased amount of focal adhesion formation was apparent in which quantifiable staining area of the cytoplasmic protein vinculin for the 200-500-nm nanofibers was 39% less compared with control, whereas the area of quantifiable vinculin staining was 45% less for both the 200-500-nm and 500-1,000-nm nanofibers. The distances of cell migration were quantified on green fluorescent protein-nucleofected cells and was 56.7%, 37.3%, and 46.3% for 50-200, 200-500, and 500-1,000 nm, respectively, compared with those on the control. Alkaline phosphatase activity demonstrated no differences after 12 days of osteogenic differentiation, and reverse transcription-polymerase chain reaction (RT-PCR) analysis showed comparable osteogenic gene expression of osteocalcin, osteonectin, and ostepontin between cells differentiated on polystyrene and nanofiber surfaces. Moreover, single-cell RT-PCR of type I collagen gene expression demonstrated higher expression on cells seeded on the nanofibers. Therefore, type I collagen nanofibers support the growth of MSCs without compromising their osteogenic differentiation capability and can be used as a scaffold for bone tissue engineering to facilitate intramembranous bone formation. Further efforts are necessary to enhance their biomimetic properties.
Susceptibility of human fetal mesenchymal stem cells to Kaposi sarcoma-associated herpesvirus. Blood 2004 NOV
Abstract
Recent reports link Kaposi sarcoma-associated herpesvirus (KSHV) infection of bone marrow cells to bone marrow failure and lymphoproliferative syndromes. The identity of the infected marrow cells, however, remains unclear. Other work has demonstrated that circulating mononuclear cells can harbor KSHV where its detection predicts the onset and severity of Kaposi sarcoma. In either setting, bone marrow precursors may serve as viral reservoirs. Since mesenchymal stem cells (MSCs) in human bone marrow regulate the differentiation and proliferation of adjacent hematopoietic precursors, we investigated their potential role in KSHV infection. Our results indicate that primary MSCs are susceptible to both cell-free and cell-associated KSHV in culture. Moreover, infection persisted within nearly half of the cells for up to 6 weeks. Thus, MSCs possess a clear capacity to support KSHV infection and warrant further exploration into their potential role in KSHV-related human disease.
更多信息
| 种属 | Human |
|---|---|
| Magnet Compatibility | |
| 样本来源 | Bone Marrow |
| Selection Method | Negative |
标记抗体
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抗人CD34抗体，克隆8G12小鼠单克隆IgG1抗体，抗人CD34
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抗人CD34抗体，克隆581小鼠单克隆IgG1抗体，抗人CD34
