Areas of Interest
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CAR T Cell Manufacturing Workflow: Isolation, Activation and Expansion Learn about human T cell isolation, activation, and expansion workflow for cell therapy
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Large-Scale Expansion of Human Pluripotent Stem Cells in 3D Suspension Culture with mTeSR™3D Medium Features and benefits of mTeSR™3D, a specialized medium for expansion of human pluripotent stem cells in suspension culture, including the novel fed-batch culture system
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Production of Chimeric Antigen Receptor T Cells This free Nature Protocols Wallchart summarizes the processes involved in producing CAR T cells for therapy.
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Setting Up Controls and Gating for ALDHbr Assay Analysis Find everything you need to know about preparing the proper single color controls, FMOs and gates for performing flow cytometry analysis of the ALDHbr assay
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How to Count 3D Sphere Cultures (Mammospheres, Neurospheres, Cancer Spheroids, and PSC Aggregates) Technique for counting 3D sphere cultures, such as mammospheres, neurospheres, or cancer spheroids
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How to Prepare and Plate Semi-Solid Medium Steps involved with preparing and plating methylcellulose-based semi-solid media (e.g. MethoCult™ and ClonaCell™)
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Bone Marrow Niches and HSC Fates A detailed reference on signaling pathways in the bone marrow and how these influence HSC fate decisions; created in partnership with Nature Reviews Immunology and Nature Reviews Molecular Cell Biology
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Culture and Analysis of Hematopoietic Progenitor Cells in CFU Assays Use these tips to better understand how the right medium can make the analysis of your hematopoietic CFU assays clearer