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SepMate™-50 (RUO)

密度梯度离心管，仅供研究使用
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产品号 #（选择产品）

产品号 #86450_C

密度梯度离心管，仅供研究使用

产品优势

  • 无需缓慢而费力地将血液加于密度梯度离心液面（例如 Ficoll-Paque™、Lymphoprep™）
  • 对于新鲜样本，将离心总时间缩短至 10 分钟，并无需关闭刹车下进行
  • 只需倾倒上清液即可快速轻松地收集分离的单核细胞
  • 可与RosetteSep™富集试剂配合使用，仅需25分钟即可分离特定细胞类型

产品组分包括

  • SepMate™-50（RUO; 产品号#86450）
    • 分装盒含四包管，每包25支
  • SepMate™-50（RUO），500支装（目录号#86460）
    • 分装盒含四包管，每包25支（目录号#86450）×5
main product photo
Try SepMate™-50 tubes for your density gradient centrifugation. Request a Sample
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  1. Lymphoprep™
    Lymphoprep™

    Density gradient medium for the isolation of mononuclear cells

  2. Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum
    Dulbecco's Phosphate Buffered Saline with 2% ...

    Cell culture buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

SepMate™ 是一种用于通过密度梯度离心法分离外周血单个核细胞（PBMCs）或特定细胞类型的管子。SepMate™管包含一个插件，作为将血液与离心液分隔开的屏障，让您无需仔细将血液（或骨髓、脐带血）铺在离心液层上。该设计使得单个核细胞的分离过程更加快速便捷，仅需离心后简单倾倒即可收集PBMCs。SepMate™ 可与 RosetteSep™ 富集试剂联合使用，可在 30 分钟内实现特定细胞类型的快速分离。

SepMate™-50专为处理4至17 mL样本设计。

SepMate™-50（RUO）（目录号#86450和#86460）的生产符合现行药品生产质量管理规范（cGMP），仅供科研使用（RUO）。

查阅SepMate™常见问题解答(FAQs)。

SepMate™-50专为处理4至17 mL样本设计。

SepMate™-50（RUO）（目录号#86450和#86460）的生产符合现行药品生产质量管理规范（cGMP），仅供科研使用（RUO）。

查阅SepMate™常见问题解答(FAQs)。

SepMate™-50专为处理4至17 mL样本设计。

SepMate™-50（RUO）（目录号#86450和#86460）的生产符合现行药品生产质量管理规范（cGMP），仅供科研使用（RUO）。

查阅SepMate™常见问题解答(FAQs)。

SepMate™-50专为处理4至17 mL样本设计。

SepMate™-50（RUO）（目录号#86450和#86460）的生产符合现行药品生产质量管理规范（cGMP），仅供科研使用（RUO）。

查阅SepMate™常见问题解答(FAQs)。

包含
Polypropylene tube containing an insert
 
亚型
离心管
 
细胞类型
B 细胞，树突状细胞（DCs），单核细胞，单个核细胞，NK 细胞，T 细胞，T 细胞，CD4+，T 细胞，CD8+，T 细胞，其他亚群，T 细胞，调节性细胞
 
种属
人
 
样本来源
Bone Marrow，Cord Blood，Whole Blood
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
SepMate
 
研究领域
嵌合体，HLA，免疫
 

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实验数据

PBMC recovery from fresh whole blood using SepMate™-50 versus standard density gradient centrifugation. Graph also shows PBMC recovery from a 48 hour-old sample using SepMate™. n in each group = 7

Figure 1. Recovery of mononuclear cells (MNCs) from peripheral blood using SepMate™-50 versus standard density gradient centrifiguation. Recovery of MNCs from fresh and 48-hour post blood draw enriched by density gradient centrifugation with SepMate™ (purple) or without (grey). There was no significant difference in the recovery of MNCS with and without SepMate™.

PBMC recovery from fresh whole blood using SepMate™-50 versus standard density gradient centrifugation. Graph also shows PBMC recovery from a 48 hour-old sample using SepMate™. n in each group = 7

Figure 2. Human CD4+ T Cell Isolation using SepMate™-50 and RosetteSep™ Human CD4+ T Cell Enrichment Cocktail

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
SepMate™-50 (RUO)
Catalog #
86450, 86460
Lot #
All
Language
English

相关材料与文献

技术资料 (11)

Lymphoprep™ Density Gradient Medium for Mononuclear Cell Isolation
Brochure
Lymphoprep™ Density Gradient Medium for Mononuclear Cell Isolation
SepMate™ Hassle-Free PBMCs in Just 15 Minutes
Brochure
SepMate™ Hassle-Free PBMCs in Just 15 Minutes
How to Isolate Mononuclear Cells from Blood in 15 Minutes with SepMate™
Protocol
How to Isolate Mononuclear Cells from Blood in 15 Minutes with SepMate™
Cell Isolation in One Spin Using SepMate™ Tubes and RosetteSep™ Cell Separation Reagents
1:02
Video
Cell Isolation in One Spin Using SepMate™ Tubes and RosetteSep™ Cell Separation Reagents
How SepMate™ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 Minutes
2:28
Video
How SepMate™ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 Minutes
How to Speed up Your PBMC Isolations with SepMate™
18:36
Video
How to Speed up Your PBMC Isolations with SepMate™
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
1:46
Video
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™
Scientific Poster
Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™
A Specialized Tube to Make Rosettesep™ Enrichment of Specific Cell Subsets Faster and Easier
Scientific Poster
A Specialized Tube to Make Rosettesep™ Enrichment of Specific Cell Subsets Faster and Easier
A Specialized Tube to Make Enrichment of Specific Cell Subsets Faster and Easier
Scientific Poster
A Specialized Tube to Make Enrichment of Specific Cell Subsets Faster and Easier
A Specialized Tube for Rapid PBMC Preparation, Pre-Enrichment, or Isolation of Specific Cell Populations
Scientific Poster
A Specialized Tube for Rapid PBMC Preparation, Pre-Enrichment, or Isolation of Specific Cell Populat...

文献 (27)

Knee loading repairs osteoporotic osteoarthritis by relieving abnormal remodeling of subchondral bone via Wnt/$\beta$-catenin signaling. W. Zheng et al. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 jan

Abstract

Osteoporotic osteoarthritis (OPOA) is a common bone disease mostly in the elderly, but the relationship between Osteoporotic (OP) and osteoarthritis (OA) is complex. It has been shown that knee loading can mitigate OA symptoms. However, its effects on OPOA remain unclear. In this study, we characterized pathological linkage of OP to OA, and evaluated the effect of knee loading on OPOA. We employed two mouse models (OA and OPOA), and conducted histology, cytology, and molecular analyses. In the OA and OPOA groups, articular cartilage was degenerated and Osteoarthritis Research Society International score was increased. Subchondral bone underwent abnormal remodeling, the differentiation of bone marrow mesenchymal stem cells (BMSCs) to osteoblasts and chondrocytes was reduced, and migration and adhesion of pre-osteoclasts were enhanced. Compared to the OA group, the pathological changes of OA in the OPOA group were considerably aggravated. After knee loading, however, cartilage degradation was effectively prevented, and the abnormal remodeling of subchondral bone was significantly inhibited. The differentiation of BMSCs was also improved, and the expression of Wnt/$\beta$-catenin was elevated. Collectively, this study demonstrates that osteoporosis aggravates OA symptoms. Knee loading restores OPOA by regulating subchondral bone remodeling, and may provide an effective method for repairing OPOA.
View publication
High Dimensional Mass Cytometry Analysis Reveals Characteristics of the Immunosuppressive Microenvironment in Diffuse Astrocytomas. W. Fu et al. Frontiers in oncology 2020

Abstract

The tumor immune microenvironment (TIME) plays a pivotal role in tumor development, progression, and prognosis. However, the characteristics of the TIME in diffuse astrocytoma (DA) are still unclear. Leveraging mass cytometry with a panel of 33 markers, we analyzed the infiltrating immune cells from 10 DA and 4 oligodendroglioma (OG) tissues and provided a single cell-resolution landscape of the intricate immune microenvironment. Our study profiled the composition of the TIME in DA and confirmed the presence of immune cells, such as glioma-associated microglia/macrophages (GAMs), CD8+ T cells, CD4+ T cells, regulatory T cells (Tregs), and natural killer cells. Increased percentages of PD-1+ CD8+ T cells, TIM-3+ CD4+ T cell subpopulations, Tregs and pro-tumor phenotype GAMs substantially contribute to the local immunosuppressive microenvironment in DA. DAs and OGs share similar compositions in terms of immune cells, while GAMs in DA exhibit more inhibitory characteristics than those in OG.
View publication
Identification of anti-CD16a single domain antibodies and their application in bispecific antibodies. Y. Zhao et al. Cancer biology {\&} therapy 2019 sep

Abstract

CD16a (Fc$\gamma$RIIIa) mediates the antibody dependent cellular cytotoxicity (ADCC) and is important for anti-tumor activities of many therapeutic antibodies. Bispecific antibody targeting natural killer (NK) cells has been studied for cancer therapy. In this work, anti-CD16a single-domain antibodies were identified from hCD16a immunized camel. Bispecific antibodies are then constructed by fusing these single domain antibodies with an anti-CEA single domain antibody. These bispecific antibodies can recruite NK cells to kill CEA-positive tumor cells, and inhibit tumor growth in vivo, suggesting that these anti-CD16a single domain antibodies are powerful tools to engaging NK cells for cancer therapy.
View publication
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更多信息

更多信息
种属 Human
Contains Polypropylene tube containing an insert
样本来源 Bone Marrow, Cord Blood, Whole Blood
Selection Method Negative
法律声明:

Ficoll-Paque™ PLUS is a trademark of GE Healthcare Ltd.Lymphoprep™ is a trademark of AXIS-SHIELD. 质量保证:

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