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STEMdiff™ 心肌细胞扩增试剂盒

用于扩增早期人PSC衍生心肌细胞的无血清试剂盒

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产品号 #（选择产品）

产品号 #100-1109_C

用于扩增早期人PSC衍生心肌细胞的无血清试剂盒

产品优势

通过简单易行的工作流程扩大心肌细胞产量
将 hPSC 衍生的心房肌或心室肌细胞扩增最多 70 倍
生成功能齐全的 hPSC 衍生心肌细胞，用于下游检测
生产纯度超过90%的心肌细胞

产品组分包括

STEMdiff™ 心肌细胞扩增试剂盒（目录号 #100-1109）

STEMdiff™ 心肌细胞维持基础培养基，1 x 490 mL
STEMdiff™ 心肌细胞传代补充剂（100X），1 x 2.5 mL
STEMdiff™ 心肌细胞扩增补充剂（50X），1 x 10 mL

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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

STEMdiff™ Cardiomyocyte Dissociation Kit
For dissociation of hPSC-derived cardiomyocytes
STEMdiff™ Cardiomyocyte Support Medium
Medium for thawing and culturing hPSC-derived cardiomyocytes
D-PBS (Without Ca++ and Mg++)
Dulbecco’s phosphate-buffered saline without calcium and magnesium
Falcon® Serological Pipettes, 10 mL
Sterile polystyrene pipettes for cell culture
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

使用该无血清试剂盒，可稳定扩增早期人类多能干细胞 (hPSC) 衍生的心肌细胞。该试剂盒可生成大量功能性且高纯度 hPSC 衍生心肌细胞，并与分别使用STEMdiff™ 心室心肌细胞分化试剂盒或STEMdiff™ 心房心肌细胞分化试剂盒生成的心室肌细胞或心房肌细胞兼容。与传统方法需要先扩增大量 hPSC，再进行多轮分化才能获得大量 hPSC 衍生心肌细胞的方法相比，STEMdiff™ 心肌细胞扩增试剂盒只需通过一轮心肌细胞分化即可扩增早期 hPSC 衍生心肌细胞，轻松获得数十亿个心肌细胞。使用该试剂盒扩增的早期hPSC衍生心肌细胞可保持稳定的电生理特性，并且在第5代时cTnT百分比超过90%。扩增后的hPSC衍生心肌细胞可用于高通量药物测试、组织工程和再生医学研究。

使用这款首创的试剂盒高效扩增心肌细胞，您可以节省时间和资源。

使用以下兼容的STEMdiff™心肌细胞产品创建完整、优化的工作流程：

STEMdiff™心室肌细胞分化试剂盒

STEMdiff™心房肌细胞分化试剂盒

STEMdiff™心肌细胞解离试剂盒

STEMdiff™心肌细胞支持培养基

STEMdiff™心肌细胞冻存培养基

STEMdiff™心肌细胞维持试剂盒

使用这款首创的试剂盒高效扩增心肌细胞，您可以节省时间和资源。

使用以下兼容的STEMdiff™心肌细胞产品创建完整、优化的工作流程：

STEMdiff™心室肌细胞分化试剂盒

STEMdiff™心房肌细胞分化试剂盒

STEMdiff™心肌细胞解离试剂盒

STEMdiff™心肌细胞支持培养基

STEMdiff™心肌细胞冻存培养基

STEMdiff™心肌细胞维持试剂盒

使用这款首创的试剂盒高效扩增心肌细胞，您可以节省时间和资源。

使用以下兼容的STEMdiff™心肌细胞产品创建完整、优化的工作流程：

STEMdiff™心室肌细胞分化试剂盒

STEMdiff™心房肌细胞分化试剂盒

STEMdiff™心肌细胞解离试剂盒

STEMdiff™心肌细胞支持培养基

STEMdiff™心肌细胞冻存培养基

STEMdiff™心肌细胞维持试剂盒

使用这款首创的试剂盒高效扩增心肌细胞，您可以节省时间和资源。

使用以下兼容的STEMdiff™心肌细胞产品创建完整、优化的工作流程：

STEMdiff™心室肌细胞分化试剂盒

STEMdiff™心房肌细胞分化试剂盒

STEMdiff™心肌细胞解离试剂盒

STEMdiff™心肌细胞支持培养基

STEMdiff™心肌细胞冻存培养基

STEMdiff™心肌细胞维持试剂盒

细胞类型
心肌细胞，PSC衍生

种属
人

应用
分化，扩增

品牌
STEMdiff

研究领域
疾病建模，药物发现和毒理检测，干细胞生物学，细胞治疗开发

制剂类别
无血清

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实验数据

hPSC-derived cardiomyocyte expansion using STEMdiff™ kits. Beating monolayer seen after 11 days.

Figure 1. Workflow for the Expansion of Early-Stage hPSC-Derived Cardiomyocytes Using the STEMdiff™ Cardiomyocyte Expansion Kit

Expansion of early-stage human pluripotent stem cell (hPSC)-derived cardiomyocytes begins with the differentiation of hPSCs to cardiomyocytes using the STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit or the STEMdiff™ Atrial Cardiomyocyte Differentiation Kit. On Day 11 of differentiation, a beating, confluent monolayer of early-stage cardiomyocytes can be observed. These cardiomyocytes are harvested and replated at low density to initiate expansion using STEMdiff™ Cardiomyocyte Expansion Kit. Expanding hPSC-derived cardiomyocytes can be passaged up to 5 times before switching to STEMdiff™ Cardiomyocyte Maintenance Medium for downstream assays.

Representative images: Expansion of ventricular cardiomyocytes using STEMdiff™ Passaging Medium.

Figure 2. Expanding hPSC-Derived Cardiomyocytes Are Confluent One Week After Replating with STEMdiff™ Cardiomyocyte Expansion Kit

Representative images of expanding hPSC-derived ventricular cardiomyocytes after replating at low density (Day 1) using STEMdiff™ Cardiomyocyte Passaging Medium. Cardiomyocyte confluency increases during the 7 day expansion period. On Day 7, a confluent monolayer of beating hPSC-derived cardiomyocytes is observed and the expanded hPSC-derived cardiomyocytes are ready for harvest.

Figure 3. Expansion of hPSC-Derived Cardiomyocytes Across Multiple Cell Lines with STEMdiff™ Cardiomyocyte Expansion Kit

(A) Expansion of hPSC-derived cardiomyocytes was tracked for 5 passages with 4 hPSC lines. Per passage, fold expansion is typically highest in the first 1 – 2 passages. (B) Cumulative fold expansion over 5 passages ranged from 33- to 103-fold expansion and averaged at 72-fold ± 12 (n = 15, 4 hPSC lines).

Cardiomyocyte purity tracked during expansion with STEMdiff™ Expansion Kit. High cTnT expression, 90% purity after 5 passages.

Figure 4. Cardiomyocyte Purity Increases During Expansion Using STEMdiff™ Cardiomyocyte Expansion Kit

Cardiomyocyte purity was tracked during expansion using STEMdiff™ Cardiomyocyte Expansion Kit for 5 passages on 4 hPSC lines. Throughout expansion, hPSC-derived cardiomyocytes express high levels of cardiac troponin T, and on average show an increase in cardiomyocyte purity during expansion. After 5 passages, cardiomyocyte cultures show an average cTnT purity of 90% ± 2% (n = 15).

MEA recordings of post-expanded hPSC-derived cardiomyocytes using STEMdiff™ kits. Stable beat rate observed.

Figure 5. Post-Expanded hPSC-Derived Cardiomyocytes Exhibit Typical Cardiac Electrophysiology and a Stable Beat Profile

Microelectrode Array (MEA) recordings of post-expanded hPSC-derived cardiomyocytes (using STEMdiff™ Cardiomyocyte Expansion Kit) exhibit a stable beat rate across 4 hPSC lines. The post-expanded hPSC-derived cardiomyocytes were replated onto MEA and maintained in STEMdiff™ Cardiomyocyte Maintenance Medium for 1 week. The average beat rate was 12 ± 1 beats per minute (n = 14, 4 hPSC lines).