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STEMdiff™ 心肌细胞铺板补充剂

一种用于提高人多能干细胞衍生心肌细胞在复苏和收获后的铺板效率的专用培养试剂盒

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产品号 #（选择产品）

产品号 #100-1120_C

一种用于提高人多能干细胞衍生心肌细胞在复苏和收获后的铺板效率的专用培养试剂盒

产品优势

Improve plating efficiency and double the number of hPSC-derived cardiomyocytes available for your experiments

Retain the stable electrical profile of hPSC-derived cardiomyocytes following replating

Save time and money with more efficient cardiomyocyte replating after thawing and dissociation

总览

实验数据

产品说明书及文档

应用领域

总览

使用STEMdiff™心肌细胞镀板试剂盒，分离、解冻和复制后，人多能干细胞（hPSC）衍生心肌细胞的产量翻倍。该试剂盒由STEMdiff™心肌细胞电镀补充剂和STEMdiff™心肌细胞支持培养基组成，可提高电镀效率并最大限度地减少hscs衍生心肌细胞的损失。

本试剂盒专为hpsc衍生的心肌细胞使用STEMdiff™心室心肌细胞分化试剂盒或STEMdiff™心房心肌细胞分化试剂盒。STEMdiff™心肌细胞电镀试剂盒通过加倍您的心肌细胞电镀效率，可以帮助提高您的实验吞吐量。

使用该试剂盒作为一个完整的，优化的工作流程的一部分，包括这些兼容STEMdiff™心肌细胞产品：

STEMdiff™心室心肌细胞分化试剂盒
 STEMdiff™心房心肌细胞分化试剂盒
 STEMdiff™心肌细胞分离试剂盒
 STEMdiff™心肌细胞支持培养基
 STEMdiff™心肌细胞冷冻培养基
 STEMdiff™心肌细胞维护试剂盒

包含
血清

细胞类型
心肌细胞，PSC衍生

种属
人

应用
冻存，分化，培养

品牌
STEMdiff

研究领域
疾病建模，药物发现和毒理检测，干细胞生物学，细胞治疗开发

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Data Figures

Graphs demonstrating iImproved plating: STEMdiff™ Cardiomyocyte Plating Medium (51%) vs. Support Medium (16%).

Figure 1. Increased Plating Efficiency Observed When Using hPSC-Derived Cardiomyocytes Using STEMdiff™ Cardiomyocyte Plating Kit

Plating efficiency of hPSC-derived cardiomyocytes was assessed 1 day after replating at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Across 4 hPSC lines, plating efficiency was improved when cardiomyocytes were replated in STEMdiff™ Cardiomyocyte Plating Medium (51% ± 3%, n=15) compared to STEMdiff™ Cardiomyocyte Support Medium (16% ± 1%, n=15).

Microscope images showing enhanced attachment of replated hPSC-derived cardiomyocytes using plating medium.

Figure 2. Morphology of Replated hPSC-Derived Cardiomyocytes Using STEMdiff™ Cardiomyocyte Plating Kit

Representative images of replated hPSC-derived cardiomyocytes. hPSC-derived cardiomyocytes were replated at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. 1 day after replating, hPSC-derived cardiomyocytes exhibit improved attachment when replated with STEMdiff™ Cardiomyocyte Plating Medium.

Microelectrode array shows stable beat rate of cardiomyocytes replated with this kit.

Figure 3. hPSC-Derived Cardiomyocytes Replated Using STEMdiff™ Cardiomyocyte Plating Kit Exhibit a Stable Beat Profile

Microelectrode array recordings of hPSC-derived cardiomyocytes replated using either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Cardiomyocytes were maintained for 1 week with STEMdiff™ Cardiomyocyte Maintenance Medium. Across 4 hPSC lines, cardiomyocytes retain a stable beat rate when replated using STEMdiff™ Cardiomyocyte Plating Medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet 1

Product Name

STEMdiff™ Cardiomyocyte Plating Supplement (100X)

Catalog #

100-1120

Lot #

All

Language

English

Document Type

Product Information Sheet 2

Product Name

STEMdiff™ Cardiomyocyte Plating Supplement (100X)

Catalog #

100-1120

Lot #

All

Language

English

Document Type

Safety Data Sheet

Product Name

STEMdiff™ Cardiomyocyte Plating Supplement (100X)

Catalog #

100-1120

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for hPSC-Derived Mesoderm Cell Research

Mesoderm Induction

Downstream Differentiation

Workflow Stages for Cardiac and Skeletal Muscle Research

View All