产品号 #08605_C
用于从人ES和iPS细胞衍生的神经元前体细胞生成功能性神经元的成熟试剂盒
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用于从人ES和iPS细胞衍生的神经元前体细胞生成功能性神经元的成熟试剂盒
Enzyme-free reagent for the selective detachment of neural rosettes
Serum-free medium kit for highly efficient SMAD inhibition-mediated neural induction of human ES and iPS cells
Differentiation kit for the generation of neuronal precursors from human ES and iPS cell-derived neural progenitor cells
Frozen human neural progenitor cells differentiated from the human induced pluripotent stem cell line, SCTi003-A
Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer
Compatible antibodies for purity assessment of isolated cells
STEMdiff™ 前脑神经元成熟试剂盒与 STEMdiff™ 前脑神经元分化试剂盒(目录号 #08600)配合使用,可从源自人类多能干细胞的神经祖细胞中生成混合的前脑型(FOXG1 阳性)神经元群。所获得的神经元纯度高(III 类 β-微管蛋白阳性神经元≥ 90%;GFAP 阳性星形胶质细胞< 10%),功能齐全,可在培养中长期维持。使用这些产品获得的神经元是构建人类神经发育和疾病模型、药物筛选、毒性测试和细胞疗法验证的多功能工具。
亚型
专用培养基
细胞类型
神经细胞,PSC衍生,神经元
种属
人
应用
细胞培养,鉴定,分化,功能学筛选,免疫细胞化学,表型鉴定
品牌
STEMdiff
研究领域
疾病建模,药物发现和毒理检测,神经科学
制剂类别
无血清
Figure 1. Schematic for the Embryoid Body Protocol
Forebrain-type neurons can be generated from NPCs after 6 days in STEMdiff™ Forebrain Neuron Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS. NPCs = neural progenitor cells; PIS = product information sheet
Figure 2. Schematic for the Monolayer Protocol
Forebrain-type neurons can be generated from NPCs after 6 days in STEMdiff™ Forebrain Neuron Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS. NPC = neural progenitor cells; PIS = product information sheet
Figure 3. Forebrain-Type Neurons Are Generated After Culture in STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits
NPCs generated from hPSCs in mTeSR 1™ using the STEMdiff™ SMADi Neural Induction Kit EB protocol were differentiated and matured to forebrain-type neurons using the STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits. (A) Forebrain-type neurons were formed after iPS cell-derived NPCs were cultured with the STEMdiff™ Forebrain Neuron Differentiation Kit for 7 days and STEMdiff™ Forebrain Neuron Maturation Kit for 14 days. The resulting cultures contain a highly pure population of (B) class III β-tubulin-positive neurons (green), with (C) fewer than 10% astrocytes (GFAP-positive cells, red). (D) Nuclei are labeled with DAPI (blue). NPCs = neural progenitor cells; hPSC = human pluripotent stem cell; EB = embryoid body; iPS = induced pluripotent stem
Figure 4. Downstream Differentiation of Neural Progenitor Cells to Neurons Is Possible Using the STEMdiff™ Differentiation and Maturation Kits
(A) NPCs generated from STiPS-R038 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit EB protocol were differentiated and matured to cortical neurons using STEMdiff™ Forebrain Neuron Differentiation Kit for 7 days and STEMdiff™ Forebrain Neuron Maturation Kit for 14 days. The resulting cultures contain a highly pure population of (B) class III β-tubulin-positive neurons (green) with less than 10% GFAP-positive astrocytes (not shown). (C) The generated neurons are also positive for FOXG1 expression (red), indicating a forebrain-type identity. (D) Nuclei are labeled with Hoechst (blue). NPCs = neural progenitor cells; hPSC = human pluripotent stem cell
Figure 5. A Mixed Population of Forebrain-Type Cortical Neurons Is Generated Using the STEMdiff™ Differentiation and Maturation Kits
Forebrain-type neurons generated from iPSC-derived NPCs (line AIW002-02) were cultured using the STEMdiff™ Forebrain Neuron Differentiation Kit for 7 days and subsequently matured for the following 6 weeks using STEMdiff™ Forebrain Neuron Maturation Kit. The resulting cultures contain a mixed population of neurons expressing VGLUT1, a glutamatergic marker of excitatory neurons (green), as well as MAP2-positive neurons, indicating mature neurons (magenta). Nuclei are labeled with Hoechst (blue). Data courtesy of Cecilia Rocha, The Neuro's Early Drug Discovery Unit (EDDU), McGill University. iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells
Figure 6. PSC-Derived Astrocytes and Neurons Can Be Co-Cultured to Model Cell-Cell Interactions In Vitro
NPCs generated from the H1 cell line were differentiated to astrocytes using STEMdiff™ Astrocyte Differentiation and Maturation Kits. H9 cell-derived NPCs were differentiated to forebrain-type neurons using STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits. For co-culture, matured astrocytes were seeded onto forebrain neurons that had been in STEMdiff™ Forebrain Neuron Maturation Medium for at least one week. Co-cultures were then switched to STEMdiff™ Forebrain Neuron Maturation Medium the following day and for the remaining co-culture. (A) Neurons cultured alone, following the co-culture feeding schedule, are labeled with DCX (green). (B) DCX-positive neurons (green) and astrocytes (GFAP, red) can be co-cultured for at least 1 - 2 weeks prior to analysis. For a detailed co-culture protocol, please see the Methods Library. NPCs = neural progenitor cells
Figure 7. PSC-Derived Neurons Survive and Mature when Co-Cultured with PSC-Derived Astrocytes
NPCs generated from the STiPS-R038 cell line were differentiated to astrocytes using STEMdiff™ Astrocyte Differentiation and Maturation Kits. STiPS-M001 cell-derived NPCs were differentiated to forebrain-type neurons using STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits. After co-culture for one week, neurons (A) had significantly increased neurite outgrowth as measured on MAP2-positive neurons with the NeuriteTracer plugin for ImageJ (M Pool et al. J Neurosci Methods, 2008) and (B) were more numerous than neurons cultured alone using the same feeding schedule. *, p < 0.05; **, p < 0.01. NPCs = neural progenitor cells
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种属 | Human |
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配方类别 | Serum-Free |
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