STEMdiff™ Neural Induction Medium (NIM, Catalog # 05835) is a serum-free medium for the neural induction of human pluripotent stem cells (hPSCs), including human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. The STEMdiff™ SMADi Neural Induction Kit (Catalog # 08581) combines STEMdiff™ NIM with the STEMdiff™ SMADi Neural Induction Supplement, which directs differentiation by inhibiting TGF-β/BMP-dependent SMAD signaling. Both media enable highly efficient generation of neural progenitor cells (NPCs) using either embryoid body (EB)- or monolayer culture-based protocols. The cultures generated will be enriched for central nervous system (CNS)-type NPCs, which express SOX1, Nestin, and PAX6. Below, we highlight advantages of the STEMdiff™ SMADi Neural Induction Kit as it utilizes the same mechanisms as Lorenz Studer’s Dual SMAD inhibition approach¹:

For performance data of the STEMdiff™ SMADi Neural Induction Kit, refer to the data and publications sections of the product page.

For further inquiries about this product, please contact Product and Scientific Support at techsupport@stemcell.com.