StemSpan™ T Cell Progenitor Maturation Supplement (10X)

总览

StemSpan™ T细胞祖细胞成熟添加物（10X）支持淋巴祖细胞成熟为双阳性（DP）T细胞。

此添加物可单独购买，也可作为StemSpan™ T细胞生成试剂盒或STEMdiff™ T细胞试剂盒的一部分购买。在此试剂盒的实验方案中，首先使用StemSpan™淋巴祖细胞扩增添加物（10X）将CD34+ 细胞分化为CD7+ CD5+淋巴祖细胞，然后进一步分化为DP T 细胞。StemSpan ™ T细胞祖细胞成熟添加物（10X）旨在与StemSpan™ SFEM II培养基以及StemSpan™淋巴分化包被材料（100X）包被的培养板搭配使用。同时，该培养基可以通过额外的刺激支持DP T细胞成熟为CD8 T细胞。

有关StemSpan™扩增和分化CD34+细胞的详细方案，请参阅技术手册和相关材料。

此添加物可单独购买，也可作为StemSpan™ T细胞生成试剂盒或STEMdiff™ T细胞试剂盒的一部分购买。在此试剂盒的实验方案中，首先使用StemSpan™淋巴祖细胞扩增添加物（10X）将CD34+ 细胞分化为CD7+ CD5+淋巴祖细胞，然后进一步分化为DP T 细胞。StemSpan ™ T细胞祖细胞成熟添加物（10X）旨在与StemSpan™ SFEM II培养基以及StemSpan™淋巴分化包被材料（100X）包被的培养板搭配使用。同时，该培养基可以通过额外的刺激支持DP T细胞成熟为CD8 T细胞。

有关StemSpan™扩增和分化CD34+细胞的详细方案，请参阅技术手册和相关材料。

此添加物可单独购买，也可作为StemSpan™ T细胞生成试剂盒或STEMdiff™ T细胞试剂盒的一部分购买。在此试剂盒的实验方案中，首先使用StemSpan™淋巴祖细胞扩增添加物（10X）将CD34+ 细胞分化为CD7+ CD5+淋巴祖细胞，然后进一步分化为DP T 细胞。StemSpan ™ T细胞祖细胞成熟添加物（10X）旨在与StemSpan™ SFEM II培养基以及StemSpan™淋巴分化包被材料（100X）包被的培养板搭配使用。同时，该培养基可以通过额外的刺激支持DP T细胞成熟为CD8 T细胞。

有关StemSpan™扩增和分化CD34+细胞的详细方案，请参阅技术手册和相关材料。

此添加物可单独购买，也可作为StemSpan™ T细胞生成试剂盒或STEMdiff™ T细胞试剂盒的一部分购买。在此试剂盒的实验方案中，首先使用StemSpan™淋巴祖细胞扩增添加物（10X）将CD34+ 细胞分化为CD7+ CD5+淋巴祖细胞，然后进一步分化为DP T 细胞。StemSpan ™ T细胞祖细胞成熟添加物（10X）旨在与StemSpan™ SFEM II培养基以及StemSpan™淋巴分化包被材料（100X）包被的培养板搭配使用。同时，该培养基可以通过额外的刺激支持DP T细胞成熟为CD8 T细胞。

有关StemSpan™扩增和分化CD34+细胞的详细方案，请参阅技术手册和相关材料。

亚型
添加剂

细胞类型
造血干/祖细胞

种属
人

应用
细胞培养，分化

品牌
StemSpan

研究领域
癌症，免疫，干细胞生物学，细胞治疗开发

制剂类别
无血清

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实验数据

Figure 1. Frequency and Yield of CD7⁺CD5⁺ Pro-T Cells After 14 Days of Culture

CB-derived CD34⁺ cells (freshly isolated or frozen) were cultured for 14 days in StemSpan™ SFEM II containing Lymphoid Progenitor Expansion Supplement (Catalog #09915) on plates coated with Lymphoid Differentiation Coating Material (Catalog #09925). Cells were harvested and analyzed for CD7 and CD5 expression by (A) flow cytometry. The (B) average frequency of viable CD7⁺CD5⁺ pro-T cells on day 14 was 70%, with ~200 CD7⁺CD5⁺ cells produced per input CD34⁺ cell. Shown are means with 95% confidence intervals (n = 33).

Figure 2. Frequency and Yield of CD4 ISP and CD4⁺CD8⁺ DP Cells After 42 Days of Culture

CB-derived CD34+ cells (freshly isolated or frozen) were cultured with the StemSpan™ T Cell Generation Kit (Catalog #09940) for 42 days and (A) analysed by flow cytometry for the expression of CD4, CD8, CD3 and TCRαβ. The (B) frequency and (C) yield of CD4 ISP, double-positive (CD4⁺CD8⁺) and CD3⁺TCRαβ⁺-expressing double-positive cells (CD4⁺CD8⁺CD3⁺TCRαβ⁺) are shown. On average, 38% of the total viable population were DP (CD4⁺CD8⁺), of which 35% co-expressed CD3 and TCRαβ. The yields of total DP cells and CD3⁺TCRαβ⁺ DP cells per input CD34⁺ cell were ~23,000 and ~9,000, respectively. Shown are means with 95% confidence intervals (n = 31).

Figure 3. Frequency and Yield of CD8 SP T Cells After 49 Days of Culture

DP cells were further matured into CD8 SP T cells by culturing for an additional 7 days in StemSpan™ SFEM II with T Cell Progenitor Maturation Supplement (Catalog #09930), IL-15 (Catalog #78031) and ImmunoCult™ CD3/CD28/CD2 T Cell Activator (Catalog #10970) on coated plates. On day 49, cells were (A) analyzed by flow cytometry for the expression of CD3, TCRαβ, CD4 and CD8. The (B) frequency and yield of CD3⁺TCRαβ⁺-expressing cells and their subsets are shown. On average, 54% of the CD3⁺TCRαβ⁺ cells were DP (CD4⁺CD8⁺) and 38% were CD8 SP (CD4^-CD8⁺). The average yield of CD8 SP T cells per input CD34⁺ cell was ~6,000. CD3⁺TCRαβ⁺ CD4 SP (CD4⁺CD8^-) T cells were detected at very low frequencies (data not shown). Shown are means with 95% confidence intervals (n = 12).