EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #07910_C
酶法细胞解离试剂
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总览
使用胰蛋白酶- EDTA（0.05%）制备人胚胎干细胞（ES）或人诱导多能干细胞（iPS）单细胞悬液，用于流式细胞术或克隆实验分析。EDTA削弱细胞与细胞的粘附，从而改善胰蛋白酶对靶向肽键的水解效率，并增强粘附细胞的脱离。该浓度比标准浓度(0.25%；产品号# 07901)更温和，并且除了人类胚胎干细胞和iPS细胞外，还可以应用于许多其他细胞培养系统。
包含
• 0.5 g/L Porcine trypsin
• Hanks' Balanced Salt Solution (without Ca++ and Mg++)
• 0.2 g/L EDTA•4Na
亚型
酶法相关（或酶解类产品
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
细胞培养
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
文献 (1)
Intermediate DNA methylation is a conserved signature of genome regulation Nature Communications 2015 DEC
Abstract
The role of intermediate methylation states in DNA is unclear. Here, to comprehensively identify regions of intermediate methylation and their quantitative relationship with gene activity, we apply integrative and comparative epigenomics to 25 human primary cell and tissue samples. We report 18,452 intermediate methylation regions located near 36% of genes and enriched at enhancers, exons and DNase I hypersensitivity sites. Intermediate methylation regions average 57% methylation, are predominantly allele-independent and are conserved across individuals and between mouse and human, suggesting a conserved function. These regions have an intermediate level of active chromatin marks and their associated genes have intermediate transcriptional activity. Exonic intermediate methylation correlates with exon inclusion at a level between that of fully methylated and unmethylated exons, highlighting gene context-dependent functions. We conclude that intermediate DNA methylation is a conserved signature of gene regulation and exon usage.
更多信息
| 种属 | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Contains | • 0.5 g/L Porcine trypsin • Hanks' Balanced Salt Solution (without Ca++ and Mg++) • 0.2 g/L EDTA•4Na |
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