EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #72332_C
在体外增强造血干细胞自我更新能力的嘧啶并吲哚类衍生物
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总览
UM729 是一种嘧啶并[4,5-b]-吲哚衍生物，可在体外增强人类造血干细胞的自我更新。UM729 不抑制芳烃受体 (AHR) 通路，但已被证明可与 AHR 拮抗剂协同作用，抑制培养的急性髓系白血病 (AML) 细胞分化。
维持和自我更新
·体外增强人造血干细胞的自我更新（Fares et al.）。
癌症研究:
·与 StemRegenin 1 (SR1) 协同作用，抑制培养的 AML 细胞分化（Pabst et al.）。
关于UM729的临床应用，请联系ExCellThera。
细胞类型
癌细胞及细胞系，造血干/祖细胞，白血病/淋巴瘤细胞
种属
人
应用
扩增，培养
研究领域
癌症，干细胞生物学
CAS 编号
Not applicable
化学式
C₂₀H₂₅N₅O₂ · X HCl [X H2O]
纯度
≥ 95 %
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (7)
文献 (2)
Cord blood expansion. Pyrimidoindole derivatives are agonists of human hematopoietic stem cell self-renewal. Science (New York, N.Y.) 2014 SEP
Abstract
The small number of hematopoietic stem and progenitor cells in cord blood units limits their widespread use in human transplant protocols. We identified a family of chemically related small molecules that stimulates the expansion ex vivo of human cord blood cells capable of reconstituting human hematopoiesis for at least 6 months in immunocompromised mice. The potent activity of these newly identified compounds, UM171 being the prototype, is independent of suppression of the aryl hydrocarbon receptor, which targets cells with more-limited regenerative potential. The properties of UM171 make it a potential candidate for hematopoietic stem cell transplantation and gene therapy.
Identification of small molecules that support human leukemia stem cell activity ex vivo. Nature methods 2014 APR
Abstract
Leukemic stem cells (LSCs) are considered a major cause of relapse in acute myeloid leukemia (AML). Defining pathways that control LSC self-renewal is crucial for a better understanding of underlying mechanisms and for the development of targeted therapies. However, currently available culture conditions do not prevent spontaneous differentiation of LSCs, which greatly limits the feasibility of cell-based assays. To overcome these constraints we conducted a high-throughput chemical screen and identified small molecules that inhibit differentiation and support LSC activity in vitro. Similar to reports with cord blood stem cells, several of these compounds suppressed the aryl-hydrocarbon receptor (AhR) pathway, which we show to be inactive in vivo and rapidly activated ex vivo in AML cells. We also identified a compound, UM729, that collaborates with AhR suppressors in preventing AML cell differentiation. Together, these findings provide newly defined culture conditions for improved ex vivo culture of primary human AML cells.
更多信息
| 种属 | Human |
|---|---|
| Cas Number | Not applicable |
| Chemical Formula | C₂₀H₂₅N₅O₂ · X HCl [X H2O] |
| 纯度 | ≥ 95% |
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StemRegenin 1芳烃受体（AHR）拮抗剂
