RosetteSep™ is a unique immunodensity cell isolation platform that isolates highly purified cells during density gradient centrifugation. With RosetteSep™, isolating untouched cells from human whole blood samples is fast, easy, and uses no columns or magnets.

In order to optimize your cell isolations using RosetteSep™, we recommend using the appropriate density medium. There are a number of different density media available to suit your particular needs:

Name of Gradient Medium	Catalog #	Density	Cells Isolated
Lymphoprep™ *	18060 18061 07811 07861	1.077 g/mL	Recommended for the isolation of mononuclear cells (MNCs) from peripheral blood, cord blood, and bone marrow by exploiting differences in cell density. Also suitable for the enrichment of a number of MNC subtypes using RosetteSep™.
Ficoll-Paque™		1.077 g/mL	Recommended for the isolation of mononuclear cells (MNCs) from peripheral blood, cord blood, and bone marrow by exploiting differences in cell density. Also suitable for the enrichment of a number of MNC subtypes using RosetteSep™.
RosetteSep™ DM-L Density Medium	15705	1.081 g/mL	RosetteSep™ DM-L is a density gradient medium designed specifically for the enrichment of human lymphocyte populations (CD3+ T cells, CD4+ T cells, CD8+ T cells, and B cells) from whole blood using the following with RosetteSep™ cocktails: product_link_text="Human T Cell Enrichment(Catalog #15021) product_link_text="Human CD4+ T Cell Enrichment (Catalog #15022) product_link_text="Human CD8+ T Cell Enrichment (Catalog #15023) product_link_text="Human B Cell Enrichment (Catalog #15024) product_link_text="Human Total Lymphocyte Enrichment (Catalog #15223) product_link_text="HLA Lymphoid Cell Enrichment Kit (Catalog #15271HLA)
RosetteSep™ DM-M Density Medium	15725	1.085 g/mL	RosetteSep™ DM-M is a density gradient medium designed specifically for the enrichment of human myeloid (CD33+) cells from whole blood using the following RosetteSep™ cocktail: HLA Myeloid Cell Enrichment Kit (Catalog #15272HLA)

* Lymphoprep™ is a trademark of Axis-Shield. Lymphoprep™ is equivalent to Ficoll-Paque™ in composition and has the exact same density. Lymphoprep™ can be used interchangeably with Ficoll-Paque™ for RosetteSep™ separations.

These density media can all be used in conjunction with our SepMate™ tubes, for quick and easier layering of blood, allowing for consistent and hassle-free density-based cell separation in as little as 15 minutes.

For more information, please contact us at techsupport@stemcell.com.