EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #72692_C
溶血磷脂酸（LPA）1和LPA2激动剂
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总览
1-油基溶血磷脂酸是溶血磷脂酸（LPA）中sn-1位含有油酸的一种分子。LPA可介导多种生物学反应，包括细胞增殖、平滑肌收缩、血小板聚集、神经突收缩和细胞迁移（Moolenaar）。
分化
·刺激培养的小鼠或大鼠神经祖细胞的神经元分化(Cui and Qiao; Fukushima et al.; Spohr et al.)。
·抑制人胚胎干细胞（ES）衍生的神经干细胞（NSCs）在体外形成神经球并分化为神经元（Dottori et al.）。
·刺激人脂肪组织来源的间充质干细胞在体外向肌成纤维样细胞的分化（Jeon et al.）。
别名
油酰-sn-3-甘油磷酸
细胞类型
脂肪细胞，间充质干/祖细胞，神经干/祖细胞
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
分化
研究领域
神经科学，干细胞生物学
CAS 编号
325465-93-8
化学式
C₂₁H₄₀O₇P · Na
分子量
458.5 克/摩尔
纯度
≥ 95 %
靶点
LPA
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Publications (9)
Lysophosphatidic acid inhibits neuronal differentiation of neural stem/progenitor cells derived from human embryonic stem cells. Stem cells (Dayton, Ohio) 2008 MAY
Abstract
Lysophospholipids are signaling molecules that play broad and major roles within the nervous system during both early development and neural injury. We used neural differentiation of human embryonic stem cells (hESC) as an in vitro model to examine the specific effects of lysophosphatidic acid (LPA) at various stages of neural development, from neural induction to mature neurons and glia. We report that LPA inhibits neurosphere formation and the differentiation of neural stem cells (NSC) toward neurons, without modifying NSC proliferation, apoptosis, or astrocytic differentiation. LPA acts through the activation of the Rho/ROCK and the phosphatidylinositol 3-kinase/Akt pathways to inhibit neuronal differentiation. This study is the first demonstration of a role for LPA signaling in neuronal differentiation of hESC. As LPA concentrations increase during inflammation, the inhibition of neuronal differentiation by LPA might contribute to the low level of neurogenesis observed following neurotrauma.
Lysophosphatidic acid receptor-dependent secondary effects via astrocytes promote neuronal differentiation. The Journal of biological chemistry 2008 MAR
Abstract
Lysophosphatidic acid (LPA) is a simple phospholipid derived from cell membranes that has extracellular signaling properties mediated by at least five G protein-coupled receptors referred to as LPA(1)-LPA(5). In the nervous system, receptor-mediated LPA signaling has been demonstrated to influence a range of cellular processes; however, an unaddressed aspect of LPA signaling is its potential to produce specific secondary effects, whereby LPA receptor-expressing cells exposed to, or primed�
Cancer-derived lysophosphatidic acid stimulates differentiation of human mesenchymal stem cells to myofibroblast-like cells. Stem cells (Dayton, Ohio) 2008 MAR
Abstract
Lysophosphatidic acid (LPA) is enriched in ascites of ovarian cancer patients and is involved in growth and invasion of ovarian cancer cells. Accumulating evidence suggests cancer-associated myofibroblasts play a pivotal role in tumorigenesis through secreting stromal cell-derived factor-1 (SDF-1). In the present study, we demonstrate that LPA induces expression of alpha-smooth muscle actin (alpha-SMA), a marker for myofibroblasts, in human adipose tissue-derived mesenchymal stem cells (hADSCs). The LPA-induced expression of alpha-SMA was completely abrogated by pretreatment of the cells with Ki16425, an antagonist of LPA receptors, or by silencing LPA(1) or LPA(2) isoform expression with small interference RNA (siRNA). LPA elicited phosphorylation of Smad2/3, and siRNA-mediated depletion of endogenous Smad2/3 or adenoviral expression of Smad7, an inhibitory Smad, abrogated the LPA induced expression of alpha-SMA and phosphorylation of Smad2/3. LPA-induced secretion of transforming growth factor (TGF)-beta1 in hADSCs, and pretreatment of the cells with SB431542, a TGF-beta type I receptor kinase inhibitor, or anti-TGF-beta1 neutralizing antibody inhibited the LPA-induced expression of alpha-SMA and phosphorylation of Smad2. Furthermore, ascites from ovarian cancer patients or conditioned medium from ovarian cancer cells induced expression of alpha-SMA and phosphorylation of Smad2, and pretreatment of the cells with Ki16425 or SB431542 abrogated the expression of alpha-SMA and phosphorylation of Smad2. In addition, LPA increased the expression of SDF-1 in hADSCs, and pretreatment of the cells with Ki16425 or SB431562 attenuated the LPA-stimulated expression of SDF-1. These results suggest that cancer-derived LPA stimulates differentiation of hADSCs to myofibroblast-like cells and increases SDF-1 expression through activating autocrine TGF-beta1-Smad signaling pathway.
更多信息
| Molecular Weight | 458.5 g/mol |
|---|---|
| 种属 | Human, Mouse, Non-Human Primate, Other, Rat |
| Alternative Names | Oleoyl-sn-3-glycerophosphate |
| Cas Number | 325465-93-8 |
| Chemical Formula | C₂₁H₄₀O₇P · Na |
| 纯度 | ≥ 95% |
| Target | LPA |
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