EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #05751_C
培养人神经干细胞和祖细胞的培养基
若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系
You may notice that your reagent packaging looks slightly different from images displayed here or from previous orders. Due to pandemic-related plasticware shortages, we are temporarily using alternative bottles for this product. Rest assured that the products themselves and how you should use them have not changed.
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
-
Heparin SolutionCell culture supplement
-
Human Recombinant bFGFBasic fibroblast growth factor
-
Human Recombinant EGFEpidermal growth factor
总览
NeuroCult™ NS-A 扩增试剂盒（人）是一款标准化无血清基础培养体系，用于在神经球或贴壁单层培养系统中扩增人神经干细胞和脑肿瘤干细胞。添加适当的细胞因子后，NeuroCult™ NS-A 扩增试剂盒（人）可优化培养人神经干细胞，使其在长时间培养中保持细胞活力，且不丧失其自我更新、增殖或分化潜能。
注：需添加 rh EGF（产品号 #78006）、rh bFGF（产品号 #78003）和肝素（产品号 #07980）。
注：需添加 rh EGF（产品号 #78006）、rh bFGF（产品号 #78003）和肝素（产品号 #07980）。
注：需添加 rh EGF（产品号 #78006）、rh bFGF（产品号 #78003）和肝素（产品号 #07980）。
注：需添加 rh EGF（产品号 #78006）、rh bFGF（产品号 #78003）和肝素（产品号 #07980）。
亚型
专用培养基
细胞类型
脑肿瘤干细胞，神经干/祖细胞
种属
人
应用
细胞培养，克隆筛选，扩增，功能学筛选，球状体培养，细胞毒性检测
品牌
NeuroCult
研究领域
癌症，药物发现和毒理检测，神经科学，干细胞生物学
制剂类别
无血清
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (14)
文献 (112)
Single-cell RNA-seq reveals that glioblastoma recapitulates a normal neurodevelopmental hierarchy. Nature communications 2020 jul
Abstract
Cancer stem cells are critical for cancer initiation, development, and treatment resistance. Our understanding of these processes, and how they relate to glioblastoma heterogeneity, is limited. To overcome these limitations, we performed single-cell RNA sequencing on 53586 adult glioblastoma cells and 22637 normal human fetal brain cells, and compared the lineage hierarchy of the developing human brain to the transcriptome of cancer cells. We find a conserved neural tri-lineage cancer hierarchy centered around glial progenitor-like cells. We also find that this progenitor population contains the majority of the cancer's cycling cells, and, using RNA velocity, is often the originator of the other cell types. Finally, we show that this hierarchal map can be used to identify therapeutic targets specific to progenitor cancer stem cells. Our analyses show that normal brain development reconciles glioblastoma development, suggests a possible origin for glioblastoma hierarchy, and helps to identify cancer stem cell-specific targets.
A comparison of exosomes derived from different periods breast milk on protecting against intestinal organoid injury. Pediatric surgery international 2019 dec
Abstract
AIM OF THE STUDY Human breast milk reduces the risk and severity of necrotizing enterocolitis (NEC). Exosomes are extracellular vesicles (EVs) found in high concentrations in milk, and they mediate intercellular communication and immune responses. The aim of this study is to compare the protective effects of exosomes that are derived from different time periods of breast milk production against intestinal injury using an ex vivo intestinal organoid model. METHODS Colostrum, transitional and mature breast milk samples from healthy lactating mothers were collected. Exosomes were isolated using serial ultracentrifugation and filtration. Exosomes' presence was confirmed using transmission electron microscopy (TEM) and western blot. To form the intestinal organoids, terminal ileum was harvested from neonatal mice pups at postnatal day 9, crypts were isolated and organoids were cultured in matrigel. Organoids were either cultured with exposure to lipopolysaccharide (LPS), or in treatment groups where both LPS and exosomes were added in the culturing medium. Inflammatory markers and organoids viability were evaluated. MAIN RESULTS Human milk-derived exosomes were successfully isolated and characterized. LPS administration reduced the size of intestinal organoids, induced inflammation through increasing TNF$\alpha$ and TLR4 expression, and stimulated intestinal regeneration. Colostrum, transitional and mature human milk-derived exosome treatment all prevented inflammatory injury, while exosomes derived from colostrum were most effective at reducing inflammatory cytokine. CONCLUSIONS Human breast milk-derived exosomes were able to protect intestine organoids against epithelial injury induced by LPS. Colostrum exosomes offer the best protective effect among the breast-milk derived exosomes. Human milk exosomes can be protective against the development of intestinal injury such as that seen in NEC.
BMP signaling mediates glioma stem cell quiescence and confers treatment resistance in glioblastoma Scientific Reports 2019 dec
Abstract
Despite advances in therapy, glioblastoma remains an incurable disease with a dismal prognosis. Recent studies have implicated cancer stem cells within glioblastoma (glioma stem cells, GSCs) as mediators of therapeutic resistance and tumor progression. In this study, we investigated the role of the transforming growth factor-$\beta$ (TGF-$\beta$) superfamily, which has been found to play an integral role in the maintenance of stem cell homeostasis within multiple stem cell systems, as a mediator of stem-like cells in glioblastoma. We find that BMP and TGF-$\beta$ signaling define divergent molecular and functional identities in glioblastoma, and mark relatively quiescent and proliferative GSCs, respectively. Treatment of GSCs with BMP inhibits cell proliferation, but does not abrogate their stem-ness, as measured by self-renewal and tumorigencity. Further, BMP pathway activation confers relative resistance to radiation and temozolomide chemotherapy. Our findings define a quiescent cancer stem cell population in glioblastoma that may be a cellular reservoir for tumor recurrence following cytotoxic therapy.
更多信息
| 种属 | Human |
|---|---|
| 配方类别 | Serum-Free |
相关产品
-
ALDEFLUOR™工具用于鉴定、评估和分离表达高水平ALDH的干细胞和祖细胞
-
NeuroCult™ NS-A 基础培养基（人）培养人神经干细胞和祖细胞的基础培养基
-
NeuroCult™ NS-A 分化试剂盒（人）人神经干细胞和祖细胞分化的培养基
-
用于小鼠和大鼠神经干细胞和祖细胞分化培养的试剂盒无血清，用于人神经干细胞和祖细胞的扩增培养
法律声明:
Sold under license from StemCells California, Inc. US Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922. 质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
Sold under license from StemCells California, Inc. US Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922. 质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
