EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #78003_C
碱性成纤维细胞生长因子
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总览
碱性成纤维细胞生长因子（bFGF）是成纤维细胞生长因子家族的典型成员。FGF家族中的细胞因子具有广泛的有丝分裂和细胞存活活性(Folkman & Klagsbrun；Kimelman & Kirschner)，并参与多种生物过程，包括细胞增殖、分化、存活和凋亡(Folkman & Klagsbrun；Klagsbrun;Rifkin & Moscatelli)。bFGF具有β-三叶结构（Ponting & Russell），结合4个FGF受体（FGFR）家族成员，激活JAK/STAT、PI3K、ERK1/2等人受体酪氨酸激酶（RTK）信号通路。它支持维持未分化的人胚胎干细胞(Xu 等人；Kang等人)，刺激人胚胎干细胞形成神经莲座（Zhang等人），促进人间充质干细胞增殖，增强软骨分化（Solchaga等人）。
亚型
细胞因子，生长因子
细胞类型
脑肿瘤干细胞，内胚层，PSC衍生，造血干/祖细胞，间充质干/祖细胞，中胚层，PSC衍生，神经细胞，PSC衍生，神经干/祖细胞，神经元，多能干细胞，前列腺细胞
种属
人
研究领域
上皮细胞研究，神经科学，干细胞生物学
纯度
低于95%
实验数据
产品说明书及文档
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应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (5)
文献 (2)
FACS-Free isolation and purification protocol of mouse prostate epithelial cells for organoid primary culture. MethodsX 2022
Abstract
The prostate is a gland that contributes to men's fertility. It is highly responsive to androgens and is often the site of carcinogenesis, as prostate cancer is the most frequent cancer in men in over a hundred countries. To study the normal prostate, few in vitro models exist, and most of them do not express the androgen receptor (AR). To overcome this issue, prostate epithelial cells can be grown in primary culture ex vivo in 2- and 3-dimensional culture (organoids). However, methods to purify these cells often require flow cytometry, thus necessitating specialized instruments and expertise. Herein, we present a detailed protocol for the harvest, purification, and primary culture of mouse prostate epithelial cells to grow prostate organoids ex vivo. This protocol does not require flow cytometry approaches, facilitating its implementation in most research laboratories, and organoids grown with this protocol are highly responsive to androgens. In summary, we present a new simple method that can be used to grow prostate organoids that recapitulate the androgen response of this gland in vivo.
Retinoblastoma-binding proteins 4 and 9 are important for human pluripotent stem cell maintenance. Experimental hematology 2011 AUG
Abstract
OBJECTIVE: The molecular mechanisms that maintain human pluripotent stem (PS) cells are not completely understood. Here we sought to identify new candidate PS cell regulators to facilitate future improvements in their generation, expansion, and differentiation. MATERIALS AND METHODS: We used bioinformatic analyses of multiple serial-analysis-of-gene-expression libraries (generated from human PS cells and their differentiated derivatives), together with small interfering RNA (siRNA) screening to identify candidate pluripotency regulators. Validation of candidate regulators involved promoter analyses, Affymetrix profiling, real-time PCR, and immunoprecipitation. RESULTS: Promoter analysis of genes differentially expressed across multiple serial-analysis-of-gene-expression libraries identified E2F motifs in the promoters of many PS cell-specific genes (e.g., POU5F1, NANOG, SOX2, FOXD3). siRNA analyses identified two retinoblastoma binding proteins (RBBP4, RBBP9) as required for maintenance of multiple human PS cell types. Both RBBPs were bound to RB in human PS cells, and E2F motifs were present in the promoters of genes whose expression was altered by decreasing RBBP4 and RBBP9 expression. Affymetrix and real-time PCR studies of siRNA-treated human PS cells showed that reduced RBBP4 or RBBP9 expression concomitantly decreased expression of POU5F1, NANOG, SOX2, and/or FOXD3 plus certain cell cycle genes (e.g., CCNA2, CCNB1), while increasing expression of genes involved in organogenesis (particularly neurogenesis). CONCLUSIONS: These results reveal new candidate positive regulators of human PS cells, providing evidence of their ability to regulate expression of pluripotency, cell cycle, and differentiation genes in human PS cells. These data provide valuable new leads for further elucidating mechanisms of human pluripotency.
更多信息
| 种属 | Human |
|---|---|
| 纯度 | less than 95% |
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质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
