若您需要咨询产品或有任何技术问题,请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

NeuroCult™ NS-A 分化试剂盒(人)

人神经干细胞和祖细胞分化的培养基

产品号 #(选择产品)

产品号 #05752_C

人神经干细胞和祖细胞分化的培养基

产品组分包括

  • NeuroCult™NS-A基础培养基(人),450 mL(产品号:#05750)
  • NeuroCult™ 分化添加物(人),50mL

总览

NeuroCult™NS-A分化试剂盒(人)是一种标准化培养基,用于将人神经干和祖细胞分化为神经元、星形胶质细胞和少突胶质细胞。

包含
• Serum
 
亚型
专用培养基
 
细胞类型
脑肿瘤干细胞,神经干/祖细胞
 
种属

 
应用
细胞培养,分化,功能学筛选
 
品牌
NeuroCult
 
研究领域
癌症,药物发现和毒理检测,神经科学,干细胞生物学
 

实验数据

Immunofluorescent staining to identify the differentiated cell types generated following culture of neural stem and progenitor cells in NeuroCult™ NS-A Differentiation Medium

Figure 1. Immunofluorescent Labeling to Identify the Differentiated Cell Types Generated Following Culture of Human Neural Stem and Progenitor Cells in the NeuroCult™ NS-A Differentiation Kit (Human)

A) Neurons (red) were detected with a mouse monoclonal ß-Tubulin III antibody. B) Immature oligodendrocytes (purple) were detected with a rabbit monoclonal O4 Oligodendrocyte Marker antibody. C) Astrocytes (green) were detected with a rabbit polyclonal GFAP antibody. D) Mature oligodendrocytes (purple) were detected with a galactocerebroside antibody.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
05752
Lot #
All
Language
English
Document Type
Technical Manual
Catalog #
05752
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
05752
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
05752
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (9)

文献 (13)

Humanized Stem Cell Models of Pediatric Medulloblastoma Reveal an Oct4/mTOR Axis that Promotes Malignancy M. \vCan\vcer et al. Cell Stem Cell 2019 dec

Abstract

Medulloblastoma (MB), the most frequent malignant childhood brain tumor, can arise from cellular malfunctions during hindbrain development. Here we generate humanized models for Sonic Hedgehog (SHH)-subgroup MB via MYCN overexpression in primary human hindbrain-derived neuroepithelial stem (hbNES) cells or iPSC-derived NES cells, which display a range of aggressive phenotypes upon xenografting. iPSC-derived NES tumors develop quickly with leptomeningeal dissemination, whereas hbNES-derived cells exhibit delayed tumor formation with less dissemination. Methylation and expression profiling show that tumors from both origins recapitulate hallmarks of infant SHH MB and reveal that mTOR activation, as a result of increased Oct4, promotes aggressiveness of human SHH tumors. Targeting mTOR decreases cell viability and prolongs survival, showing the utility of these varied models for dissecting mechanisms mediating tumor aggression and demonstrating the value of humanized models for a better understanding of pediatric cancers.
Cytomegalovirus infection induces a stem cell phenotype in human primary glioblastoma cells: prognostic significance and biological impact. Fornara O et al. Cell death and differentiation 2016 FEB

Abstract

Glioblastoma (GBM) is associated with poor prognosis despite aggressive surgical resection, chemotherapy, and radiation therapy. Unfortunately, this standard therapy does not target glioma cancer stem cells (GCSCs), a subpopulation of GBM cells that can give rise to recurrent tumors. GBMs express human cytomegalovirus (HCMV) proteins, and previously we found that the level of expression of HCMV immediate-early (IE) protein in GBMs is a prognostic factor for poor patient survival. In this study, we investigated the relation between HCMV infection of GBM cells and the presence of GCSCs. Primary GBMs were characterized by their expression of HCMV-IE and GCSCs marker CD133 and by patient survival. The extent to which HCMV infection of primary GBM cells induced a GCSC phenotype was evaluated in vitro. In primary GBMs, a large fraction of CD133-positive cells expressed HCMV-IE, and higher co-expression of these two proteins predicted poor patient survival. Infection of GBM cells with HCMV led to upregulation of CD133 and other GSCS markers (Notch1, Sox2, Oct4, Nestin). HCMV infection also promoted the growth of GBM cells as neurospheres, a behavior typically displayed by GCSCs, and this phenotype was prevented by either chemical inhibition of the Notch1 pathway or by treatment with the anti-viral drug ganciclovir. GBM cells that maintained expression of HCMV-IE failed to differentiate into neuronal or astrocytic phenotypes. Our findings imply that HCMV infection induces phenotypic plasticity of GBM cells to promote GCSC features and may thereby increase the aggressiveness of this tumor.
Rapid and Efficient Direct Conversion of Human Adult Somatic Cells into Neural Stem Cells by HMGA2/let-7b. Yu K-R et al. Cell reports 2015 JAN

Abstract

A recent study has suggested that fibroblasts can be converted into mouse-induced neural stem cells (miNSCs) through the expression of defined factors. However, successful generation of human iNSCs (hiNSCs) has proven challenging to achieve. Here, using microRNA (miRNA) expression profile analyses, we showed that let-7 microRNA has critical roles for the formation of PAX6/NESTIN-positive colonies from human adult fibroblasts and the proliferation and self-renewal of hiNSCs. HMGA2, a let-7-targeting gene, enables induction of hiNSCs that displayed morphological/molecular features and in vitro/in vivo differentiation potential similar to H9-derived NSCs. Interestingly, HMGA2 facilitated the efficient conversion of senescent somatic cells or blood CD34+ cells into hiNSCs through an interaction with SOX2, whereas other combinations or SOX2 alone showed a limited conversion ability. Taken together, these findings suggest that HMGA2/let-7 facilitates direct reprogramming toward hiNSCs in minimal conditions and maintains hiNSC self-renewal, providing a strategy for the clinical treatment of neurological diseases.

更多信息

更多信息
种属 Human
Contains • Serum
法律声明:

Sold under license from StemCells California, Inc. US Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922. 质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息,请访问 STEMCELL.CN/COMPLIANCE.
Copyright © 2025 by STEMCELL Technologies. All rights reserved.