EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #07921_C
细胞分离溶液
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总览
使用 ACCUMAX™ 可将细胞团块解离为单细胞悬液，以提高细胞计数的准确性。这款即用型溶液含有蛋白水解酶和胶原酶活性，能够有效分散细胞团块，并有效促进细胞从原代组织以及标准和粘附涂层培养塑料器皿上分离。
ACCUMAX™ 不含任何哺乳动物或细菌来源成分，确保实验结果的可重复性和可靠性，已验证可广泛应用于多种细胞类型。每批次产品均通过以下质量检测：无菌性（采用 USP 膜过滤法）、酶活性（使用合成显色四肽）以及细胞从组织培养塑料器皿上的分离性测试。
包含
• 1X ACCUMAX™ enzymes in Dulbecco’s phosphate-buffered saline (D-PBS)
• 0.5 mM EDTA•4Na
亚型
酶法相关（或酶解类产品
细胞类型
多能干细胞
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
细胞培养
研究领域
干细胞生物学
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
相关材料与文献
文献 (1)
Transcriptome Dynamics of Developing Photoreceptors in Three-Dimensional Retina Cultures Recapitulates Temporal Sequence of Human Cone and Rod Differentiation Revealing Cell Surface Markers and Gene Networks. Stem cells (Dayton, Ohio) 2015 DEC
Abstract
The derivation of three-dimensional (3D) stratified neural retina from pluripotent stem cells has permitted investigations of human photoreceptors. We have generated a H9 human embryonic stem cell subclone that carries a green fluorescent protein (GFP) reporter under the control of the promoter of cone-rod homeobox (CRX), an established marker of postmitotic photoreceptor precursors. The CRXp-GFP reporter replicates endogenous CRX expression in vitro when the H9 subclone is induced to form self-organizing 3D retina-like tissue. At day 37, CRX+ photoreceptors appear in the basal or middle part of neural retina and migrate to apical side by day 67. Temporal and spatial patterns of retinal cell type markers recapitulate the predicted sequence of development. Cone gene expression is concomitant with CRX, whereas rod differentiation factor neural retina leucine zipper protein (NRL) is first observed at day 67. At day 90, robust expression of NRL and its target nuclear receptor NR2E3 is evident in many CRX+ cells, while minimal S-opsin and no rhodopsin or L/M-opsin is present. The transcriptome profile, by RNA-seq, of developing human photoreceptors is remarkably concordant with mRNA and immunohistochemistry data available for human fetal retina although many targets of CRX, including phototransduction genes, exhibit a significant delay in expression. We report on temporal changes in gene signatures, including expression of cell surface markers and transcription factors; these expression changes should assist in isolation of photoreceptors at distinct stages of differentiation and in delineating coexpression networks. Our studies establish the first global expression database of developing human photoreceptors, providing a reference map for functional studies in retinal cultures.
更多信息
| 种属 | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Contains | • 1X ACCUMAX™ enzymes in Dulbecco’s phosphate-buffered saline (D-PBS) • 0.5 mM EDTA•4Na |
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法律声明:
ACCUMAX is a trademark of Innovative Cell Technologies, Inc. 质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
ACCUMAX is a trademark of Innovative Cell Technologies, Inc. 质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
