抗人CD105抗体，克隆43A3

抗人、小鼠CD105（内皮糖蛋白）的小鼠单克隆IgG1抗体

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产品号 #60039_C

抗人、小鼠CD105（内皮糖蛋白）的小鼠单克隆IgG1抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

43A3抗体与CD105（内皮糖蛋白）反应，CD105是一种~180kDa的细胞表面糖蛋白，是由分子量约为90 kDa的I型跨膜亚基组成的二硫键同型二聚体。CD105是TGF-β受体复合物的一个组成部分，在血管内皮平滑肌细胞、胎盘的合体滋养细胞和活化的巨噬细胞中表达，在基质成纤维细胞中表达水平相对较低。在某些类型的肿瘤中也可以观察到它的表达，并且在血管生成过程中内皮细胞的CD105表达水平上调。CD105与信号受体协同作用，高亲和力结合TGF-β1和TGF-β3，但不结合TGF-β2。研究发现，其他配体包括活化素A、BMP-2和BMP-7。CD105在血管生成、心血管发育和血管重塑中发挥重要作用，该蛋白通过调节黏着斑和细胞迁移位点，在细胞骨架重组中发挥调控作用。CD105的某些突变会导致常染色体显性遗传病-遗传性出血性毛细血管扩张症。该抗体克隆已验证可用于标记在MesenCult™增殖试剂盒（人；产品号 #05411)和MesenCult™-XF培养基（产品号#05420）中培养的人间充质细胞。

亚型
一抗

靶抗原
CD105 (Endoglin)

别名
内皮素

活性物种
人，小鼠

偶联
Alexa Fluor 488，APC，Biotin 或生物素，FITC，PE，未偶联的

宿主物种
小鼠

细胞类型
造血干/祖细胞，间充质干/祖细胞

种属
人，小鼠

应用
流式细胞术，免疫沉淀，Western印迹

研究领域
内皮细胞研究，干细胞生物学

克隆
43A3

基因编号
2022

同种型
IgG1，kappa

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实验数据

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD105 Antibody, Clone 43A3, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human PBMCs cultured for 24 hours with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, Alexa Fluor® 488. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, Alexa Fluor® 488 is shown (solid line histogram).

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD105 Antibody, Clone 43A3, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human PBMCs cultured for 24 hours with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, PE. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, PE is shown (solid line histogram).

Figure 3. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) cultured for 24 hours with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, followed by a rat anti-mouse IgG1 antibody, PE. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, followed by a rat anti-mouse IgG1 antibody, PE is shown (solid line histogram).

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) cultured for 24 h with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, APC. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, APC is shown in the bottom panel (solid line histogram).

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) cultured for 24 h with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC is shown in the bottom panel (solid line histogram).

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) cultured for 24 h with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, FITC. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, FITC is shown in the bottom panel (solid line histogram).