产品号 #60110_C
抗人、恒河猴、白眉猴CD123 (IL-3Rα)的小鼠单克隆IgG1抗体
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6H6抗体可与人CD123 (IL-3受体α亚基)反应,CD123是一种约70 kDa的I型跨膜糖蛋白,属于I型细胞因子受体家族(5型亚家族)和免疫球蛋白(Ig)超家族。CD123构成异二聚体IL-3受体的配体结合α链。CD123本身与IL-3的结合亲和力较低,但当与CD131(受体的信号转导β链)结合时,可以观察到IL-3的高亲和力结合。CD123在造血祖细胞、内皮细胞、嗜碱性粒细胞、嗜酸性粒细胞、肥大细胞、单核细胞、巨噬细胞、树突状细胞、巨核细胞、一个B细胞亚群以及在粒细胞-巨噬细胞集落刺激因子 (GM-CSF) 存在下培养的中性粒细胞中表达。IL-3结合CD123可刺激造血细胞的增殖、分化和活力。CD123在恶性肿瘤如急性白血病中高度表达。6H6抗体不抑制IL-3与CD123或IL-3受体的结合。
亚型
一抗
靶抗原
CD123 (IL-3Rα)
别名
hIL-3Ra,IL3R,IL3RAX,IL3RAY,IL3RX,IL3RY,IL-3Ra,人 IL‑3 受体 α 链(SP2亚型),人 IL‑3 受体 α 链,MGC34174
活性物种
人,Rhesus,Sooty Mangabey
偶联
APC,Biotin 或 生物素,FITC,PE,PerCP-Cyanine5.5,未偶联的
宿主物种
小鼠
细胞类型
树突状细胞(DCs),粒细胞及其亚群,造血干/祖细胞,髓系细胞
种属
人,非人灵长类
应用
CyTOF,流式细胞术,功能学筛选,免疫细胞化学,免疫荧光,免疫组化,免疫沉淀,Western印迹
研究领域
免疫
克隆
6H6
基因编号
3563
同种型
IgG1,kappa
Figure 1. Flow Cytometry Analysis of Human PBMCs
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, followed by a rat anti-mouse IgG1 antibody, PE and Anti-Human CD45RO Antibody, Clone UCHL1, APC (Catalog #60097AZ). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60071) followed by a rat anti-mouse IgG1 antibody, PE and Anti-Human CD45RO Antibody, Clone UCHL1, APC (Catalog #60097AZ).
Figure 2. Data for APC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, APC and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60071AZ) and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE).
Figure 3. Data for Biotin-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, Biotin followed by streptavidin (SAV) APC and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin (Catalog #60071BT) followed by SAV APC and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE).
Figure 4. Data for FITC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, FITC and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60071FI) and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE).
Figure 5. Data for PE-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, PE and Anti-Human CD45RO Antibody, Clone UCHL1, APC (Catalog #60097AZ). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60071PE) and Anti-Human CD45RO Antibody, Clone UCHL1, APC (Catalog #60097AZ). (C) Flow cytometry analysis (gated on CD45+) of human whole blood nucleated cells processed with the EasySep™ Human Basophil Enrichment Kit (Catalog #19069) and labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, PE and an anti-IgE antibody, FITC. Labeling of the Start cells prior to cell isolation is shown (D).
Figure 6. Data for PerCP-Cy55-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, PerCP-Cy5.5 and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PerCPCy5.5 (Catalog #60070PS) and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE).
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种属 | Human, Non-Human Primate |
---|---|
克隆 | 6H6 |
Gene Id | 3563 |
Alternative Names | hIL-3Ra, IL3R, IL3RAX, IL3RAY, IL3RX, IL3RY, IL-3Ra, IL-3 receptor alpha SP2 isoform, Interleukin-3 Receptor alpha, MGC34174 |
同种型/isotype | IgG1, kappa |
免疫磁珠负选试剂盒
小鼠单克隆IgG1, kappa同型对照抗体
小鼠单克隆IgG1, kappa同型对照抗体
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