抗人CD3抗体，克隆OKT3，FITC

抗人CD3的小鼠单克隆IgG2a抗体

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产品号 #100-0285_C

抗人CD3的小鼠单克隆IgG2a抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

OKT3抗体可特异性识别人T细胞受体（TCR）/CD3复合物中约20 kDa的CD3ε亚基上的胞外构象表位，该表位在大多数（约95%）成熟T细胞和NKT细胞表面表达，在胸腺细胞上也有不同程度的表达，大多数T细胞肿瘤也表达CD3。CD3是由CD3γ、δ、ε、η和ζ亚基组成的二聚体，这些亚基在结构上属于免疫球蛋白超家族的1型跨膜蛋白。CD3与TCR非共价结合，介导抗原识别信号向T细胞胞内的转导，调控T细胞活化及TCR的膜表面表达。CD3基因突变与免疫系统疾病有关，包括重症联合免疫缺陷症（SCID）。 OKT3抗体可识别CD3ε亚基与CD3γ或CD3δ结合时表达的表位，该抗体的结合可诱导T细胞激活。由于其在体内具有免疫抑制特性，OKT3抗体已被证明可用于治疗同种异体移植排斥反应。克隆OKT3被广泛用于人T细胞表型分析，并已观察到其可阻断抗CD3抗体克隆SK7和UCHT1的结合。

亚型
一抗

靶抗原
CD3

别名
CD3 epsilon，CD3ε，Leu-4，T3

活性物种
人

偶联
APC，FITC，未偶联的

宿主物种
小鼠

细胞类型
T 细胞

应用
流式细胞术，功能学筛选，免疫组化，Western印迹

研究领域
免疫

克隆
OKT3

基因编号
916

同种型
IgG2a，kappa

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实验数据

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells labeled with Anti-Human CD3 Antibody, Clone OKT3, APC or a mouse IgG2a, kappa APC isotype control antibody.

Figure 1. Data for Anti-Human CD3 Antibody, Clone OKT3, APC

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone OKT3, APC (filled histogram) or a mouse IgG2a, kappa APC isotype control antibody (solid line histogram). Viable lymphocytes were gated for analysis.

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells labeled with Anti-Human CD3 Antibody, Clone OKT3, FITC or a mouse IgG2a, kappa FITC isotype control antibody.

Figure 2. Data for Anti-Human CD3 Antibody, Clone OKT3, FITC

Flow cytometry analysis of human PBMCs labeled with Anti-Human CD3 Antibody, Clone OKT3, FITC (filled histogram) or a mouse IgG2a, kappa FITC isotype control antibody (solid line histogram). Viable lymphocytes were gated for analysis.

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells labeled with Anti-Human CD3 Antibody, Clone OKT3, followed by a goat anti-mouse IgG2a antibody, PE or a mouse IgG2a, kappa isotype control antibody, followed by a goat anti-mouse IgG2a antibody, PE.

Figure 3. Data for Anti-Human CD3 Antibody, Clone OKT3, Unconjugated

Flow cytometry analysis of human PBMCs labeled with Anti-Human CD3 Antibody, Clone OKT3, followed by a goat anti-mouse IgG2a antibody, PE (filled histogram) or a mouse IgG2a, kappa isotype control antibody, followed by a goat anti-mouse IgG2a antibody, PE (solid line histogram). Viable lymphocytes were gated for analysis.