Anti-Human CD4, Clone RPA-T4| STEMCELL Technologies

总览

RPA-T4抗体可特异性识别CD4。CD4是一种分子量约为59 kDa的单链1型跨膜糖蛋白，属于免疫球蛋白 (Ig) 超级家族。CD4包含四个胞外免疫球蛋白样结构域 (D1-D4)。RPA-T4识别该蛋白的D1结构域，其结构类似于免疫球蛋白可变结构域。CD4在大多数胸腺细胞和T细胞亚群（辅助性T细胞）中表达水平相对较高，而在外周血单个核细胞和巨噬细胞中表达水平较低。CD4与MHC II的非多态性区域结合，并通过增强TCR和MHC II抗原复合物之间的亲和力，在MHC II限制性抗原识别中作为T细胞受体（TCR）的共受体。CD4还通过胞内结构域与细胞质酪氨酸激酶（如Lck）的相互作用，放大从TCR到细胞质的信号。此外，CD4是人类免疫缺陷病毒（HIV）的受体。RPA-T4抗体的结合可阻断HIV结合和混合淋巴细胞反应（MLR），并通过干扰CD4与HLA-DR的结合来抑制体外CD4+ T细胞的激活。它也能部分阻断SK3抗体的结合。研究发现，RPA-T4抗体的表位对甲醛固定敏感。

亚型
一抗

靶抗原
CD4

别名
Leu3，L3T4，T4

活性物种
Chimpanzee，人

偶联
APC，FITC，PE，未偶联的

宿主物种
小鼠

细胞类型
T 细胞

应用
CyTOF，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹

研究领域
免疫

克隆
RPA-T4

基因编号
920

同种型
IgG1，kappa

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实验数据

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, APC or a mouse IgG1, kappa APC isotype control antibody.

Figure 1. Data for Anti-Human CD4 Antibody, Clone RPA-T4, APC

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, FITC or a mouse IgG1, kappa FITC isotype control antibody.

Figure 2. Data for Anti-Human CD4 Antibody, Clone RPA-T4, FITC

Flow cytometry analysis of human PBMCs labeled with Anti-Human CD4 Antibody, Clone RPA-T4, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, PE or a mouse IgG1, kappa PE isotype control antibody.

Figure 3. Data for Anti-Human CD4 Antibody, Clone RPA-T4, PE

Flow cytometry analysis of human PBMCs labeled with Anti-Human CD4 Antibody, Clone RPA-T4, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, followed by a goat anti-mouse IgG1 antibody, PE or a mouse IgG1, kappa isotype control antibody, followed by a goat anti-mouse IgG1 antibody, PE.

Figure 4. Data for Anti-Human CD4 Antibody, Clone RPA-T4, Unconjugated

Flow cytometry analysis of human PBMCs labeled with Anti-Human CD4 Antibody, Clone RPA-T4, followed by a goat anti-mouse IgG1 antibody, PE (filled histogram) or a mouse IgG1, kappa isotype control antibody, followed by a goat anti-mouse IgG1 antibody, PE (solid line histogram).