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抗人CD45RA，克隆HI100

抗人、黑猩猩CD45RA的小鼠单克隆IgG2b抗体
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产品号 #100-0316_C

抗人、黑猩猩CD45RA的小鼠单克隆IgG2b抗体

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总览
实验数据
产品说明书及文档
应用领域
相关产品

总览

HI100抗体可特异性识别CD45的CD45RA异构体上的细胞外表位。CD45是一种I型跨膜糖蛋白，属于蛋白酪氨酸磷酸酶家族（受体1/6亚家族）。编码CD45胞外RA、RB和RC多肽的外显子4、5和6发生可变剪接，产生几种分子量为180 - 240 kDa的同种异构体。切除外显子4可产生分子量约为220 kDa的CD45RA亚型，该亚型在naïve T细胞、B细胞和单核细胞亚群以及骨髓胸腺细胞中表达。CD45RA通过T细胞受体和B细胞受体增强信号传导。HI100抗体通常与抗CD45RO异构体的抗体联合使用，以区分naïve T细胞和记忆T细胞，并可用于识别CD4+淋巴细胞中的抑制/诱导亚群。CD45RA+ T细胞的比例通常随着年龄的增长而下降。

亚型
一抗
 
靶抗原
CD45RA
 
别名
B220，CD45，GP180，Lyt-4，Ly-5，LY5，L-CA，LCA，蛋白酪氨酸磷酸酶受体型C，PTPRC，T200
 
活性物种
Chimpanzee，人
 
偶联
APC-Cyanine7，Biotin 或 生物素，PE，未偶联的
 
宿主物种
小鼠
 
细胞类型
粒细胞及其亚群，造血干/祖细胞，白细胞，淋巴细胞
 
应用
CyTOF，ELISAs，FACS，流式细胞术，功能学筛选，免疫亲和色谱，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，放射免疫分析，Western印迹
 
研究领域
免疫
 
克隆
HI100
 
基因编号
5788
 
同种型
IgG2b，kappa
 

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实验数据

Figures showing flow cytometry analysis of human peripheral blood mononuclear cells (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, APC-Cyanine7 and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa isotype control antibody, clone MPC-11, APC-Cyanine7 and anti-human CD45RO antibody, clone UCHL1, APC and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, APC-Cyanine7 or a mouse IgG2b, kappa APC-Cyanine7 isotype control antibody.

Figure 1. Data for Anti-Human CD45RA Antibody, Clone HI100, APC-Cy7

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, APC-Cyanine7 and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa isotype control antibody, clone MPC-11, APC-Cyanine7 and anti-human CD45RO antibody, clone UCHL1, APC, and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, APC-Cyanine7 (filled histogram) or a mouse IgG2b, kappa APC-Cyanine7 isotype control antibody (solid line histogram).

Figures showing flow cytometry analysis of (A) human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RA Antibody, Clone HI100, Biotin, followed by streptavidin (SAV) PE, and anti-human CD45RO antibody, clone UCHL1, APC,(B) human PBMCs labeled with a mouse IgG2b, kappa Biotin isotype control antibody followed by SAV PE, and anti-human CD45RO antibody, clone UCHL1, APC, and (C) human PBMCs labeled with Anti-Human CD45RA Antibody, Clone HI100, Biotin, followed by SAV PE or a mouse IgG2b, kappa Biotin isotype control antibody, followed by SAV PE.

Figure 2. Data for Anti-Human CD45RA Antibody, Clone HI100, Biotin

Flow cytometry analysis of human PBMCs (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, Biotin, followed by streptavidin (SAV) PE, and anti-human CD45RO antibody, clone UCHL1, APC. (B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG2b, kappa Biotin isotype control antibody followed by SAV PE, and anti-human CD45RO antibody, clone UCHL1, APC. (C) Flow cytometry analysis of human PBMCs labeled with Anti-Human CD45RA Antibody, Clone HI100, Biotin, followed by SAV PE (filled histogram) or a mouse IgG2b, kappa Biotin isotype control antibody, followed by SAV PE (solid line histogram). Viable lymphocytes were gated for analysis.

Figures showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, PE, and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa PE isotype control antibody and anti-human CD45RO antibody, clone UCHL1, APC, and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, PE or a mouse IgG2b, kappa PE isotype control antibody.

Figure 3. Data for Anti-Human CD45RA Antibody, Clone HI100, PE

Flow cytometry analysis of human PBMCs (gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, PE, and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa PE isotype control antibody and anti-human CD45RO antibody, clone UCHL1, APC, and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, PE (filled histogram) or a mouse IgG2b, kappa PE isotype control antibody (solid line histogram).

Figures showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, followed by a goat anti-mouse IgG2b, PE, and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa isotype control antibody, Clone MPC-11, followed by a goat anti-mouse IgG2b, PE, and anti-human CD45RO antibody, clone UCHL1, APC, and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, followed by a goat anti-mouse IgG2b, PE or a mouse IgG2b, kappa isotype control antibody followed by a goat anti-mouse IgG2b, PE.

Figure 4. Data for Anti-Human CD45RA Antibody, Clone HI100, Unconjugated

Flow cytometry analysis of human PBMCs (gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, followed by a goat anti-mouse IgG2b, PE, and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa isotype control antibody, Clone MPC-11, followed by a goat anti-mouse IgG2b, PE, and anti-human CD45RO antibody, clone UCHL1, APC,and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, followed by a goat anti-mouse IgG2b, PE (filled histogram) or a mouse IgG2b, kappa isotype control antibody followed by a goat anti-mouse IgG2b, PE (solid line histogram).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RA, Clone HI100, APC-Cyanine7
Catalog #
100-0317, 100-0316
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RA, Clone HI100, Biotin
Catalog #
100-0322
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RA, Clone HI100, PE
Catalog #
100-0320, 100-0319
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RA, Clone HI100
Catalog #
100-0318
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RA, Clone HI100, APC-Cyanine7
Catalog #
100-0317, 100-0316
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RA, Clone HI100, Biotin
Catalog #
100-0322
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RA, Clone HI100, PE
Catalog #
100-0320, 100-0319
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RA, Clone HI100
Catalog #
100-0318
Lot #
All
Language
English

更多信息

更多信息
克隆 HI100
Gene Id 5788
Alternative Names B220, CD45, GP180, Lyt-4, Ly-5, LY5, L-CA, LCA, Protein tyrosine phosphatase receptor type C, PTPRC, T200
同种型/isotype IgG2b, kappa
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